Selected organism

Reactions on models are mapped to KEGG and BioCyc databases, either to the entire database or to a subgroup that represents a specific organism.

Model	Selected organism on KEGG	Selected organism on BioCyc
e_coli_core	Escherichia coli K-12 MG1655 (1997)	Escherichia coli K-12 substr. MG1655 (Tier 1)
iAF1260	Escherichia coli K-12 MG1655 (1997)	Escherichia coli K-12 substr. MG1655 (Tier 1)

Compartments

Model compartments are typically used to differentiate the extracellular from the intracellular environment, and can be used to further define intracellular compartments.

Model	Number of compartments
e_coli_core	2
iAF1260	2

Metabolites

Two sources are checked for finding synonyms: the "annotation" field of each metabolite in the SBML model, and, using the metabolite ID, the MNXref database is queried. The synonyms found using both methods are consolidated.

Model	Total	bigg	biocyc	cas	chebi	chemspider	drugbank	envipath	hmdb	inchi	inchi_key	kegg	knapsack	lipidmaps	metabolights	metacyc	metanetx	nci	pubchem	reactome	rhea	sabiork	seed	slm	No synonyms
e_coli_core	72	72	70	0	72	0	0	32	69	0	72	71	0	10	0	70	72	0	0	61	0	70	72	3	0
iAF1260	1668	1668	1396	0	1354	0	0	264	974	0	1387	1279	0	215	0	1396	1668	0	0	678	0	1052	1524	115	0

Reactions

Reactions Types

Reactions in the models are classified into six categories:
Metabolic reactions: convert internal metabolites into other internal metabolites. Examples: A(internal) <-> B(internal); A(internal) -> B(internal)
Exchange reactions: provide (or remove) metabolites to the extracellular compartment, simulating the external media. Examples: A (external) <-> NULL; NULL -> A (external)
Sink reactions: Similar to the exchange reactions, but the metabolites are made available directly in the intracellular compartment. Examples: A (internal) <-> NULL; NULL -> A (internal)
Transport reactions: responsible for the transport of metabolites across compartments. Examples: A (external) <-> A (internal); A (internal) -> A (external)
Demand reactions: Remove metabolites from the system. Example: A (internal) -> NULL
Deactivated reactions: can not carry flux because their minimum and maximum flux boundaries are set to zero.

Model	Total	Metabolic rxns	Exchange rxns	Sink rxns	Transport rxns	Demand rxns	Deactivated rxns
e_coli_core	95	50	7	0	25	13	0
iAF1260	2382	1355	22	0	719	282	4

Metabolic reactions blocked

Blocked reactions can not carry flux because the model cannot produce the reaction's substrate. It is dependent on the defined external media. The growth media in this analysis consists of: the default media in the model, plus all carbon, nitrogen, phosphorus, and sulfur media used in Biolog experiments.
e_coli_core: 0
iAF1260: 219

Metabolic reactions synonyms on other databases

For finding synonyms for a reaction/enzyme, the reaction ID from every reaction is used to query the MNXref database, and synonyms on the “annotation” field are extracted.

Model	Total	bigg	biocyc	cas	chebi	chemspider	drugbank	envipath	hmdb	inchi	inchi_key	kegg	knapsack	lipidmaps	metabolights	metacyc	metanetx	nci	pubchem	reactome	rhea	sabiork	seed	slm	No synonyms
e_coli_core	50	50	38	0	0	0	0	0	0	0	0	46	0	0	0	33	50	0	0	19	46	40	46	0	0
iAF1260	1355	1355	671	0	0	0	0	0	0	0	0	753	0	0	0	551	1346	0	0	193	795	521	1069	0	0

The table below shows how reactions in the model maps with reactions and conversion steps on KEGG Pathway Modules. The result is divided into six categories for each module:
Organism expected: The number of processing steps in the database present in the model (completness of pathway). With mouse over: the reactions in the model mapped to KEGG, and the number of reactions.
Organism missing: The number of reactions in the database not present in the model. With mouse over: the reactions present in the model mapped to KEGG, and the number of reactions.
Unpredicted: for some organisms, the reference database expects only part of a module to be present. This category receives the number of processing steps present in the module, but not expected according to the database.
Entire module: Sum of 'Organism expected' and 'Unpredicted'.
Blocked: number of blocked reactions in pathway.
Essentials: number of essential reactions in pathway.
By clicking on the table's links, the KEGG reactions and metabolites present in the model are highlighted in a KEGG Module map.
Units used in the table: The entry "Annotated: 2 (67%)" can be read as "2 conversion steps (67% pathway completeness)". "Processing steps" is usually equivalent to "reactions". However, in some cases, different reactions, or a combination of reactions, can be used to perform the same conversion step. For example, in the TCA cycle, 2-Oxoglutarate should be produced using Isocitrate as the substrate. This single reaction step can be performed by reactions R00709, or R00267, or (R01899 and R00268).
Regarding reactions mapping: For mapping model reactions against KEGG reactions, two sources are used: (i) checking for information on KEGG reaction synonyms stored in the SBML model, and (ii) the MNXref database. Synonyms found in (i) and (ii) are consolidated.
Color code:
Green ▶: Complete pathways, or pathways where all conversion steps predicted by the database are present in the model.
Yellow ▶: Incomplete pathways, but with at least one conversion step present in the model.
Pink ▶: Incomplete pathways, with no conversion steps present in the model.
Blue ▶: Pathways not present in the selected organism, but MinPath suggested its presence.
White: Pathways suggested to exist in another model.

	Carbohydrate metabolism
	Central carbohydrate metabolism												Other carbohydrate metabolism
	PRPP biosynthesis, ribose 5P => PRPP	Pentose phosphate pathway (Pentose phosphate cycle)	Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate	Pentose phosphate pathway, oxidative phase, glucose 6P => ribulose 5P	Glycolysis, core module involving three-carbon compounds	Glycolysis (Embden-Meyerhof pathway), glucose => pyruvate	Pyruvate oxidation, pyruvate => acetyl-CoA	Citrate cycle (TCA cycle, Krebs cycle)	Gluconeogenesis, oxaloacetate => fructose-6P	Entner-Doudoroff pathway, glucose-6P => glyceraldehyde-3P + pyruvate	Citrate cycle, first carbon oxidation, oxaloacetate => 2-oxoglutarate	Pentose phosphate pathway, non-oxidative phase, fructose 6P => ribose 5P	D-Galacturonate degradation (bacteria), D-galacturonate => pyruvate + D-glyceraldehyde 3P	Nucleotide sugar biosynthesis, galactose => UDP-galactose	Ascorbate biosynthesis, plants, fructose-6P => ascorbate	Glycogen biosynthesis, glucose-1P => glycogen/starch	Methylaspartate cycle	Ascorbate degradation, ascorbate => D-xylulose-5P	Undecaprenylphosphate alpha-L-Ara4N biosynthesis, UDP-GlcA => undecaprenyl phosphate alpha-L-Ara4N	Propanoyl-CoA metabolism, propanoyl-CoA => succinyl-CoA	Photorespiration	Glucuronate pathway (uronate pathway)	Glyoxylate cycle	D-galactonate degradation, De Ley-Doudoroff pathway, D-galactonate => glycerate-3P	Galactose degradation, Leloir pathway, galactose => alpha-D-glucose-1P	Nucleotide sugar biosynthesis, glucose => UDP-glucose	Glycogen degradation, glycogen => glucose-6P	D-Glucuronate degradation, D-glucuronate => pyruvate + D-glyceraldehyde 3P
e_coli_core	Annotated: 0 Annot. missing: 1 (100%)R01049 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (60%)R01529, R01830, R02035, R01827, R01056, R01528 (6 reactions) Annot. missing: 4 (40%)R02740, R02736, R02739, R10907, R01641 (5 reactions) Unpredicted: 0 Entire module: 6 (60%)R01529, R01830, R02035, R01827, R01056, R01528 (6 reactions) Blocked rxns: 0 Essential rxns: 1R01056	Annotated: 3 (43%)R01082, R00342, R00405 (3 reactions) Annot. missing: 4 (57%)R00621, R03316, R07618, R02164, R02570 (5 reactions) Unpredicted: 0 Entire module: 3 (38%)R01082, R00342, R00405 (3 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (67%)R01528, R02035 (2 reactions) Annot. missing: 1 (33%)R02736, R10907 (2 reactions) Unpredicted: 0 Entire module: 2 (67%)R01528, R02035 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (100%)R01015, R00200, R01512, R01518, R01061, R00658 (6 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 6 (86%)R01015, R00200, R01512, R01518, R01061, R00658 (6 reactions) Blocked rxns: 0 Essential rxns: 4R01512, R01061, R00658, R01518	Annotated: 6 (60%)R01015, R00200, R01512, R01518, R01061, R00658 (6 reactions) Annot. missing: 4 (40%)R01786, R04779, R02740, R01070 (4 reactions) Unpredicted: 0 Entire module: 6 (54%)R01015, R00200, R01512, R01518, R01061, R00658 (6 reactions) Blocked rxns: 0 Essential rxns: 4R01512, R01061, R00658, R01518	Annotated: 1 (20%)R00209 (1 reactions) Annot. missing: 4 (80%)R02569, R07618, R03270, R00014 (4 reactions) Unpredicted: 0 Entire module: 1 (20%)R00209 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (60%)R01325, R01082, R00405, R00342, R00351, R01900, R00267 (7 reactions) (R01325, R01900 are present but can not be highlighted on KEGG map) Annot. missing: 4 (40%)R00621, R03316, R07618, R02164, R02570 (5 reactions) Unpredicted: 0 Entire module: 6 (54%)R01325, R01082, R00405, R00342, R00351, R01900, R00267 (7 reactions) Blocked rxns: 0 Essential rxns: 4R01325, R01900, R00267, R00351	Annotated: 6 (75%)R01015, R01512, R01518, R00341, R01061, R00658 (6 reactions) Annot. missing: 2 (25%)R01070, R04780 (2 reactions) Unpredicted: 0 Entire module: 6 (75%)R01015, R01512, R01518, R00341, R01061, R00658 (6 reactions) Blocked rxns: 0 Essential rxns: 4R01512, R01061, R00658, R01518	Annotated: 1 (25%)R02035 (1 reactions) Annot. missing: 3 (75%)R02736, R02036, R05605 (3 reactions) Unpredicted: 0 Entire module: 1 (25%)R02035 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)R01325, R01900, R00267, R00351 (4 reactions) (R01325, R01900 are present but can not be highlighted on KEGG map) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)R01325, R01900, R00267, R00351 (4 reactions) Blocked rxns: 0 Essential rxns: 4R01325, R01900, R00267, R00351	Annotated: 4 (80%)R01827, R01529, R01830, R01056 (4 reactions) Annot. missing: 1 (20%)R01641 (1 reactions) Unpredicted: 0 Entire module: 4 (80%)R01827, R01529, R01830, R01056 (4 reactions) Blocked rxns: 0 Essential rxns: 1R01056	Annotated: 0 Annot. missing: 5 (100%)R05605, R01540, R01541, R01983, R02555 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 2 (100%)R01092, R00955 (2 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 4 (100%)R02421, R00289, R02110, R00948 (4 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1R00248 (1 reactions) Entire module: 5 (36%)R01325, R00248, R00342, R00351, R01900 (5 reactions) Blocked rxns: 0 Essential rxns: 3R01325, R01900, R00351	Annotated: 0 Annot. missing: 5 (100%)R07125, R05850, R07671, R07677, R03244 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 5 (100%)R07660, R07661, R07659, R07662, R07658 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 1 (100%)R00833 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (100%)R01325, R00479, R00342, R00351, R00472, R01900 (6 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 6 (100%)R01325, R00479, R00342, R00351, R00472, R01900 (6 reactions) Blocked rxns: 0 Essential rxns: 3R01325, R01900, R00351	Annotated: 2 (40%)R01512, R01061 (2 reactions) Annot. missing: 3 (60%)R01064, R03387, R03033 (3 reactions) Unpredicted: 0 Entire module: 2 (40%)R01512, R01061 (2 reactions) Blocked rxns: 0 Essential rxns: 2R01512, R01061	Annotated: 0 Annot. missing: 4 (100%)R01092, R00955, R10619, R00291 (4 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R01786, R00289, R00959 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 2 (100%)R08639, R02111 (2 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 5 (100%)R05605, R02454, R05606, R01482, R01541 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 1 (100%)R01049 (1 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)R01049 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (60%)R01529, R01830, R02035, R01827, R01056, R01528 (6 reactions) Annot. missing: 4 (40%)R02740, R02736, R02739, R10907, R01641 (5 reactions) Unpredicted: 0 Entire module: 6 (60%)R01529, R01830, R02035, R01827, R01056, R01528 (6 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (43%)R01082, R00342, R00405, R00361 (4 reactions) Annot. missing: 4 (57%)R00621, R03316, R07618, R02164, R02570 (5 reactions) Unpredicted: 0 Entire module: 3 (38%)R01082, R00342, R00405, R00361 (4 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (67%)R01528, R02035 (2 reactions) Annot. missing: 1 (33%)R02736, R10907 (2 reactions) Unpredicted: 0 Entire module: 2 (67%)R01528, R02035 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (100%)R01015, R00200, R01512, R01518, R01061, R00658 (6 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 6 (86%)R01015, R00200, R01512, R01518, R01061, R00658 (6 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (60%)R01015, R00200, R01512, R01518, R01061, R00658 (6 reactions) Annot. missing: 4 (40%)R01786, R04779, R02740, R01070 (4 reactions) Unpredicted: 0 Entire module: 6 (54%)R01015, R00200, R01512, R01518, R01061, R00658 (6 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (20%)R00209 (1 reactions) Annot. missing: 4 (80%)R02569, R07618, R03270, R00014 (4 reactions) Unpredicted: 0 Entire module: 1 (20%)R00209 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (60%)R01325, R01082, R00405, R00342, R00351, R01900, R00267, R00361 (8 reactions) (R01325, R01900 are present but can not be highlighted on KEGG map) Annot. missing: 4 (40%)R00621, R03316, R07618, R02164, R02570 (5 reactions) Unpredicted: 0 Entire module: 6 (54%)R01325, R01082, R00405, R00342, R00351, R01900, R00267, R00361 (8 reactions) Blocked rxns: 0 Essential rxns: 4R01325, R01900, R00267, R00351	Annotated: 6 (75%)R01015, R01512, R01518, R00341, R01061, R00658 (6 reactions) Annot. missing: 2 (25%)R01070, R04780 (2 reactions) Unpredicted: 0 Entire module: 6 (75%)R01015, R01512, R01518, R00341, R01061, R00658 (6 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (75%)R05605, R02036, R02035 (3 reactions) Annot. missing: 1 (25%)R02736 (1 reactions) Unpredicted: 0 Entire module: 3 (75%)R02036, R05605, R02035 (3 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)R01325, R01900, R00267, R00351 (4 reactions) (R01325, R01900 are present but can not be highlighted on KEGG map) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)R01325, R01900, R00267, R00351 (4 reactions) Blocked rxns: 0 Essential rxns: 4R01325, R01900, R00267, R00351	Annotated: 4 (80%)R01827, R01529, R01830, R01056 (4 reactions) Annot. missing: 1 (20%)R01641 (1 reactions) Unpredicted: 0 Entire module: 4 (80%)R01827, R01529, R01830, R01056 (4 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (100%)R05605, R01540, R01541, R01983, R02555 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)R05605, R01540, R01541, R01983, R02555 (5 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)R01092, R00955 (2 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)R01092, R00955 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2R01818, R01819 (2 reactions) Entire module: 2 (25%)R01818, R01819 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (50%)R00289, R00948 (2 reactions) Annot. missing: 2 (50%)R02421, R02110 (2 reactions) Unpredicted: 0 Entire module: 2 (40%)R00289, R00948 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1R00248 (1 reactions) Entire module: 5 (36%)R01325, R00248, R00342, R00351, R01900 (5 reactions) Blocked rxns: 0 Essential rxns: 3R01325, R01900, R00351	Annotated: 4 (80%)R07125, R05850, R03244, R07677 (4 reactions) Annot. missing: 1 (20%)R07671 (1 reactions) Unpredicted: 0 Entire module: 4 (80%)R07125, R05850, R03244, R07677 (4 reactions) Blocked rxns: 2R07125, R07677 Essential rxns: 0	Annotated: 3 (60%)R07660, R07659, R07658 (3 reactions) Annot. missing: 2 (40%)R07662, R07661 (2 reactions) Unpredicted: 0 Entire module: 3 (60%)R07660, R07659, R07658 (3 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)R00833 (1 reactions) Annot. missing: 0 (0%) Unpredicted: 1R02765 (1 reactions) Entire module: 2 (67%)R00833, R02765 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 4R01388, R01221, R01514, R01334 (4 reactions) Entire module: 5 (50%)R01221, R01514, R01334, R01388, R00945 (5 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 4R01476, R01639, R01381, R00286 (4 reactions) Entire module: 4 (36%)R01476, R01639, R01381, R00286 (4 reactions) Blocked rxns: 2R01476, R01381 Essential rxns: 0	Annotated: 6 (100%)R01325, R00479, R00342, R00351, R00472, R01900 (6 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 6 (100%)R01325, R00479, R00342, R00351, R00472, R01900 (6 reactions) Blocked rxns: 0 Essential rxns: 3R01325, R01900, R00351	Annotated: 5 (100%)R01512, R01064, R01061, R03387, R03033 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)R01512, R01064, R01061, R03387, R03033 (5 reactions) Blocked rxns: 3R01064, R03387, R03033 Essential rxns: 0	Annotated: 3 (75%)R01092, R00955, R00291 (3 reactions) Annot. missing: 1 (25%)R10619 (1 reactions) Unpredicted: 0 Entire module: 3 (75%)R01092, R00955, R00291 (3 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (33%)R00289 (1 reactions) Annot. missing: 2 (67%)R01786, R00959 (2 reactions) Unpredicted: 0 Entire module: 1 (33%)R00289 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (50%)R08639 (1 reactions) Annot. missing: 1 (50%)R02111 (1 reactions) Unpredicted: 0 Entire module: 1 (50%)R08639 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (100%)R05605, R02454, R05606, R01482, R01541 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)R05605, R02454, R05606, R01482, R01541 (5 reactions) Blocked rxns: 0 Essential rxns: 0

	Energy metabolism
	ATP synthesis			Carbon fixation								Methane metabolism			Nitrogen metabolism		Sulfur metabolism
	NADH:quinone oxidoreductase, prokaryotes	Fumarate reductase, prokaryotes	Succinate dehydrogenase, prokaryotes	Reductive pentose phosphate cycle, glyceraldehyde-3P => ribulose-5P	CAM (Crassulacean acid metabolism), light	CAM (Crassulacean acid metabolism), dark	C4-dicarboxylic acid cycle, NAD - malic enzyme type	Reductive acetyl-CoA pathway (Wood-Ljungdahl pathway)	Reductive citrate cycle (Arnon-Buchanan cycle)	Phosphate acetyltransferase-acetate kinase pathway, acetyl-CoA => acetate	Incomplete reductive citrate cycle, acetyl-CoA => oxoglutarate	Formaldehyde assimilation, ribulose monophosphate pathway	Methanogenesis, acetate => methane	Formaldehyde assimilation, serine pathway	Denitrification, nitrate => nitrogen	Dissimilatory nitrate reduction, nitrate => ammonia	Assimilatory sulfate reduction, sulfate => H2S
e_coli_core	Annotated: 0 Annot. missing: 1 (100%)R11945 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 1 (100%)R02164 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 1 (100%)R02164 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (67%)R00762, R01056, R01067, R01068 (4 reactions) Annot. missing: 2 (33%)R01641, R01829 (2 reactions) Unpredicted: 0 Entire module: 4 (57%)R00762, R01056, R01067, R01068 (4 reactions) Blocked rxns: 0 Essential rxns: 1R01056	Annotated: 1 (100%)R00216 (1 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (50%)R00216 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)R00342, R00345 (2 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)R00342, R00345 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1R00214 (1 reactions) Entire module: 3 (38%)R00342, R00214, R00345 (3 reactions) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2R00402, R00199 (2 reactions) Entire module: 9 (60%)R01325, R01082, R00405, R00345, R00342, R00199, R01900, R00402, R00267 (9 reactions) Blocked rxns: 0 Essential rxns: 3R01325, R01900, R00267	Annotated: 2 (100%)R00315, R00230 (2 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)R00315, R00230 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1R10660 (1 reactions) Entire module: 4 (57%)R01082, R10660, R00342, R00405 (4 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1R00756 (1 reactions) Entire module: 2 (25%)R00756, R01068 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Entire module: 2 (29%)R00315, R00230 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Entire module: 3 (30%)R00342, R00345, R00658 (3 reactions) Blocked rxns: 0 Essential rxns: 1R00658	Annotated: 0 Annot. missing: 2 (100%)R00798, R03071 (2 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 2 (100%)R05712, R09497, R00787, R00798, R03071 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 4 (100%)R00858, R00509, R02021, R00529 (4 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 0 Annot. missing: 1 (100%)R11945 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 1 (100%)R02164 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 1 (100%)R02164 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (67%)R00762, R01056, R01067, R01068 (4 reactions) Annot. missing: 2 (33%)R01641, R01829 (2 reactions) Unpredicted: 0 Entire module: 4 (57%)R00762, R01056, R01067, R01068 (4 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)R00216 (1 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (50%)R00216 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)R00342, R00345 (2 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)R00342, R00345 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 5R00258, R00355, R00214 (3 reactions) Entire module: 7 (88%)R00355, R00345, R00258, R00342, R00214 (5 reactions) Blocked rxns: 0 Essential rxns: 2R00258, R00355	Unpredicted: 1R07168 (1 reactions) Entire module: 3 (43%)R01220, R01655, R07168 (3 reactions) Blocked rxns: 0 Essential rxns: 1R07168	Unpredicted: 2R00402, R00199 (2 reactions) Entire module: 9 (60%)R01325, R01082, R00405, R00345, R00342, R00199, R01900, R00402, R00267 (9 reactions) Blocked rxns: 0 Essential rxns: 3R01325, R01900, R00267	Annotated: 2 (100%)R00315, R00230 (2 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)R00315, R00230 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1R10660 (1 reactions) Entire module: 4 (57%)R01082, R10660, R00342, R00405 (4 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1R00756 (1 reactions) Entire module: 2 (25%)R00756, R01068 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1R00235 (1 reactions) Entire module: 3 (43%)R00315, R00230, R00235 (3 reactions) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2R01388, R08572 (2 reactions) Entire module: 6 (60%)R00345, R00342, R01388, R08572, R00945, R00658 (6 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 2 (100%)R00798, R03071 (2 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (50%)R00787 (1 reactions) Annot. missing: 1 (50%)R03071, R00798, R09497 (3 reactions) Unpredicted: 0 Entire module: 1 (50%)R00787 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (100%)R00858, R00509, R02021, R00529 (4 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)R00858, R00509, R02021, R00529 (4 reactions) Blocked rxns: 0 Essential rxns: 3R00858, R00509, R00529

	Lipid metabolism
	Fatty acid metabolism				Lipid metabolism
	beta-Oxidation	Fatty acid biosynthesis, initiation	Fatty acid biosynthesis, elongation	beta-Oxidation, acyl-CoA synthesis	Phosphatidylethanolamine (PE) biosynthesis, PA => PS => PE
e_coli_core	Annotated: 0 Annot. missing: 27 (100%)R04738, R04748, R03026, R03857, R01177, R03990, R04739, R03858, R04746, R04745, R04741, R04744, R04751, R00238, R01279, R04754, R03778, R04749, R03991, R04170, R04747, R01175, R04742, R04740, R01975, R04737, R03777 (27 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R01626, R10707, R00742 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 28 (100%)R04952, R04953, R04968, R04726, R04958, R04954, R04355, R04966, R04533, R04970, R04428, R04430, R04955, R04963, R04956, R04429, R04568, R04965, R04964, R04543, R04969, R04962, R04961, R04959, R04544, R04725, R04536, R04537, R04566, R04535, R04960, R04967, R04957, R04724, R04534 (35 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 1 (100%)R01280 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R02055, R01799, R01800 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 27 (100%)R04738, R04748, R03857, R03026, R01177, R03990, R04739, R03858, R04746, R04745, R04741, R04744, R04751, R00238, R01279, R04754, R03778, R04749, R03991, R04170, R04747, R01175, R04742, R04740, R01975, R04737, R03777 (27 reactions) Annot. missing: 0 (0%) Unpredicted: 1R04743 (1 reactions) Entire module: 28 (100%)R04738, R04748, R03026, R03857, R01177, R03990, R04739, R03858, R04746, R04745, R04741, R04744, R04751, R00238, R01279, R04754, R03778, R04749, R03991, R04170, R04747, R01175, R04742, R04740, R01975, R04743, R04737, R03777 (28 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)R01626, R10707, R00742 (3 reactions) Annot. missing: 0 (0%) Unpredicted: 2R04355, R01624 (2 reactions) Entire module: 5 (100%)R00742, R01624, R04355, R01626, R10707 (5 reactions) Blocked rxns: 0 Essential rxns: 2R01626, R00742	Annotated: 15 (54%)R04964, R04543, R04536, R04969, R04534, R04537, R04953, R04566, R04535, R04954, R04355, R04568, R04965, R04544, R04724, R04970, R04725 (17 reactions) Annot. missing: 13 (46%)R04962, R04952, R04968, R04961, R04726, R04966, R04958, R04533, R04959, R04428, R04430, R04955, R04963, R04956, R04429, R04960, R04967, R04957 (18 reactions) Unpredicted: 0 Entire module: 15 (54%)R04964, R04543, R04536, R04969, R04534, R04537, R04953, R04566, R04535, R04954, R04355, R04568, R04965, R04544, R04724, R04970, R04725 (17 reactions) Blocked rxns: 0 Essential rxns: 7R04536, R04537, R04953, R04566, R04535, R04954, R04534	Annotated: 0 Annot. missing: 1 (100%)R01280 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R02055, R01799, R01800 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	Nucleotide metabolism
	Purine metabolism				Pyrimidine metabolism
	Adenine ribonucleotide biosynthesis, IMP => ADP,ATP	Inosine monophosphate biosynthesis, PRPP + glutamine => IMP	Purine degradation, xanthine => urea	Guanine ribonucleotide biosynthesis, IMP => GDP,GTP	Pyrimidine ribonucleotide biosynthesis, UMP => UDP/UTP,CDP/CTP	Uridine monophosphate biosynthesis, glutamine (+ PRPP) => UMP	Pyrimidine degradation, uracil => beta-alanine, thymine => 3-aminoisobutanoate	Pyrimidine deoxyribonuleotide biosynthesis, CDP => dCTP
e_coli_core	Annotated: 2 (50%)R00127, R00200 (2 reactions) Annot. missing: 2 (50%)R01135, R01083 (2 reactions) Unpredicted: 0 Entire module: 2 (50%)R00127, R00200 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 11 (100%)R01072, R04559, R04560, R04463, R04325, R01127, R04144, R04591, R04208, R07404, R07405 (11 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 4 (100%)R00330, R00430, R00332, R01130, R01231, R01230 (6 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 4 (100%)R00158, R00573, R00156, R00571, R00570 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 6 (100%)R01397, R00575, R01868, R00965, R01993, R01870 (6 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 4 (100%)R02269, R00977, R11026, R03055 (4 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 2 (100%)R02326, R02024 (2 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 4 (100%)R00127, R00200, R00124, R01083, R01135 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0R00333 (1 reactions) Entire module: 4 (100%)R00127, R00333, R00200, R00124, R01083, R01135 (6 reactions) Blocked rxns: 0 Essential rxns: 2R01135, R01083	Annotated: 9 (82%)R01072, R04559, R04560, R04463, R04325, R01127, R04144, R04591, R07405 (9 reactions) Annot. missing: 2 (18%)R07404, R04208 (2 reactions) Unpredicted: 0 Entire module: 9 (75%)R01072, R04559, R04463, R04560, R04325, R01127, R04144, R04591, R07405 (9 reactions) Blocked rxns: 0 Essential rxns: 8R01072, R04559, R04560, R04463, R01127, R04144, R04591, R07405	Unpredicted: 1R02103 (1 reactions) Entire module: 1 (17%)R02103 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (100%)R00330, R00430, R00332, R01130, R01231 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)R00330, R00430, R00332, R01130, R01231 (5 reactions) Blocked rxns: 0 Essential rxns: 2R01231, R00332	Annotated: 4 (100%)R00570, R00158, R00573, R00156 (4 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)R00570, R00158, R00573, R00156 (4 reactions) Blocked rxns: 0 Essential rxns: 3R00158, R00573, R00156	Annotated: 6 (100%)R01397, R00575, R01868, R00965, R01993, R01870 (6 reactions) Annot. missing: 0 (0%) Unpredicted: 0R01869, R01867 (2 reactions) Entire module: 6 (100%)R01397, R00575, R01868, R00965, R01869, R01867, R01993, R01870 (8 reactions) Blocked rxns: 0 Essential rxns: 4R00965, R01397, R01870, R01993	Annotated: 0 Annot. missing: 4 (100%)R02269, R00977, R11026, R03055 (4 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)R02326, R02024 (2 reactions) Annot. missing: 0 (0%) Unpredicted: 0R02100, R02093, R02094, R02325, R02101 (5 reactions) Entire module: 2 (100%)R02100, R02101, R02093, R02094, R02326, R02325, R02024 (7 reactions) Blocked rxns: 0 Essential rxns: 0

	Amino acid metabolism
	Arginine and proline metabolism				Aromatic amino acid metabolism					Branched-chain amino acid metabolism				Cysteine and methionine metabolism					Histidine metabolism	Lysine metabolism	Other amino acid metabolism		Polyamine biosynthesis			Serine and threonine metabolism
	Ornithine biosynthesis, glutamate => ornithine	Proline biosynthesis, glutamate => proline	Arginine biosynthesis, ornithine => arginine	Arginine succinyltransferase pathway, arginine => glutamate	Phenylalanine biosynthesis, chorismate => phenylpyruvate => phenylalanine	Tryptophan biosynthesis, chorismate => tryptophan	Shikimate pathway, phosphoenolpyruvate + erythrose-4P => chorismate	Tyrosine biosynthesis, chorismate => HPP => tyrosine	Trans-cinnamate degradation, trans-cinnamate => acetyl-CoA	Leucine biosynthesis, 2-oxoisovalerate => 2-oxoisocaproate	Leucine degradation, leucine => acetoacetate + acetyl-CoA	Valine/isoleucine biosynthesis, pyruvate => valine / 2-oxobutanoate => isoleucine	Isoleucine biosynthesis, threonine => 2-oxobutanoate => isoleucine	Methionine biosynthesis, aspartate => homoserine => methionine	Cysteine biosynthesis, serine => cysteine	Methionine degradation	Methionine salvage pathway	Cysteine biosynthesis, methionine => cysteine	Histidine biosynthesis, PRPP => histidine	Lysine biosynthesis, succinyl-DAP pathway, aspartate => lysine	GABA (gamma-Aminobutyrate) shunt	Glutathione biosynthesis, glutamate => glutathione	Polyamine biosynthesis, arginine => ornithine => putrescine	Polyamine biosynthesis, arginine => agmatine => putrescine => spermidine	GABA biosynthesis, prokaryotes, putrescine => GABA	Threonine biosynthesis, aspartate => homoserine => threonine	Betaine biosynthesis, choline => betaine	Serine biosynthesis, glycerate-3P => serine	Ectoine biosynthesis, aspartate => ectoine
e_coli_core	Annotated: 0 Annot. missing: 5 (100%)R02649, R00669, R03443, R00259, R02283 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R01251, R00239, R03313 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R01086, R01398, R01954 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 5 (100%)R05049, R04189, R00411, R00832, R04217 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R01715, R00694, R01373 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 5 (100%)R00986, R03508, R00985, R03509, R02722, R01073 (6 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 7 (100%)R03084, R01714, R01826, R03083, R03460, R02413, R02412 (7 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R01715, R00734, R01728 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (12%)R00228 (1 reactions) Annot. missing: 7 (88%)R00750, R06788, R06783, R02601, R06785, R06789, R06787 (7 reactions) Unpredicted: 0 Entire module: 1 (11%)R00228 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R01213, R03968, R04426, R04001 (4 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 2 (100%)R07618, R01090 (2 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 11 (100%)R04440, R05069, R01214, R04441, R00226, R04439, R05068, R05071, R05070, R08648, R02199 (11 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 6 (100%)R05069, R05068, R00996, R05070, R08648, R02199 (6 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 7 (100%)R01286, R02291, R01777, R00946, R04405, R03260, R01773, R00480, R01775 (9 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 2 (100%)R00586, R00897 (2 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 10 (100%)R04035, R01071, R03012, R04640, R01163, R03013, R04037, R03457, R04558, R03243 (10 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 9 (100%)R02291, R02735, R04198, R10147, R04199, R00451, R04365, R02734, R04475, R00480 (10 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R00713, R00714, R00261, R01648 (4 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 2 (100%)R00497, R00894 (2 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 1 (100%)R00670 (1 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 4 (100%)R00178, R00566, R01157, R01920 (4 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 4 (100%)R07415, R07419, R07414, R07417, R07418 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 5 (100%)R02291, R01771, R01466, R01773, R00480, R01775 (6 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 2 (100%)R01025, R02565, R02566 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R01513, R04173, R00582 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 2 (100%)R00480, R02291 (2 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 5 (100%)R02649, R00669, R03443, R00259, R02283 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)R02649, R00669, R03443, R00259, R02283 (5 reactions) Blocked rxns: 0 Essential rxns: 5R02649, R00669, R03443, R00259, R02283	Annotated: 3 (100%)R01251, R00239, R03313 (3 reactions) Annot. missing: 0 (0%) Unpredicted: 1 Entire module: 4 (100%)R01251, R00239, R03313 (3 reactions) Blocked rxns: 0 Essential rxns: 1R01251	Annotated: 3 (100%)R01086, R01398, R01954 (3 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)R01954, R01086, R01398 (3 reactions) Blocked rxns: 0 Essential rxns: 3R01086, R01398, R01954	Annotated: 5 (100%)R05049, R04189, R00411, R00832, R04217 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)R05049, R04189, R00411, R00832, R04217 (5 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)R01715, R00694, R01373 (3 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)R01715, R01373, R00694 (3 reactions) Blocked rxns: 0 Essential rxns: 3R01715, R00694, R01373	Annotated: 5 (100%)R00986, R03508, R03509, R02722, R01073 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)R00986, R03508, R03509, R02722, R01073 (5 reactions) Blocked rxns: 0 Essential rxns: 4R03508, R03509, R00986, R01073	Annotated: 7 (100%)R03084, R01714, R01826, R03083, R03460, R02413, R02412 (7 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 7 (100%)R03084, R01714, R01826, R03083, R03460, R02413, R02412 (7 reactions) Blocked rxns: 0 Essential rxns: 7R03084, R01714, R01826, R03083, R03460, R02413, R02412	Annotated: 3 (100%)R01715, R00734, R01728 (3 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)R01715, R00734, R01728 (3 reactions) Blocked rxns: 0 Essential rxns: 3R01715, R00734, R01728	Annotated: 7 (88%)R00750, R06788, R00228, R06783, R02601, R06785, R06787 (7 reactions) Annot. missing: 1 (12%)R06789 (1 reactions) Unpredicted: 0 Entire module: 7 (78%)R00750, R06788, R00228, R06783, R02601, R06785, R06787 (7 reactions) Blocked rxns: 6R00750, R06788, R06783, R02601, R06785, R06787 Essential rxns: 0	Annotated: 3 (100%)R01213, R03968, R04426, R04001 (4 reactions) (R03968, R04426, R04001 are present but can not be highlighted on KEGG map) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)R01213, R04426, R03968, R04001 (4 reactions) Blocked rxns: 0 Essential rxns: 4R01213, R03968, R04426, R04001	Annotated: 1 (50%)R01090 (1 reactions) Annot. missing: 1 (50%)R07618 (1 reactions) Unpredicted: 0 Entire module: 1 (12%)R01090 (1 reactions) Blocked rxns: 0 Essential rxns: 1R01090	Annotated: 7 (64%)R04441, R01214, R00226, R04439, R05070, R08648, R02199 (7 reactions) Annot. missing: 4 (36%)R05069, R05071, R04440, R05068 (4 reactions) Unpredicted: 0 Entire module: 7 (64%)R04441, R01214, R00226, R04439, R05070, R08648, R02199 (7 reactions) Blocked rxns: 0 Essential rxns: 6R04441, R00226, R04439, R05070, R08648, R02199	Annotated: 4 (67%)R05070, R08648, R00996, R02199 (4 reactions) Annot. missing: 2 (33%)R05069, R05068 (2 reactions) Unpredicted: 0 Entire module: 4 (67%)R05070, R08648, R00996, R02199 (4 reactions) Blocked rxns: 0 Essential rxns: 4R05070, R08648, R00996, R02199	Annotated: 7 (100%)R01286, R02291, R01777, R00946, R03260, R00480, R01775 (7 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 7 (100%)R01286, R02291, R01777, R00946, R03260, R00480, R01775 (7 reactions) Blocked rxns: 0 Essential rxns: 7R01286, R02291, R01777, R00946, R03260, R00480, R01775	Annotated: 2 (100%)R00586, R00897 (2 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)R00586, R00897 (2 reactions) Blocked rxns: 0 Essential rxns: 2R00586, R00897	Unpredicted: 2R00177, R04858 (2 reactions) Entire module: 2 (50%)R00177, R04858 (2 reactions) Blocked rxns: 1R04858 Essential rxns: 1R00177	Unpredicted: 6R01401, R00177, R07392, R04420, R04143, R07396 (6 reactions) Entire module: 8 (62%)R01401, R00177, R07392, R01920, R00178, R04420, R04143, R07396 (8 reactions) Blocked rxns: 7R01401, R07392, R01920, R00178, R04420, R04143, R07396 Essential rxns: 1R00177	Unpredicted: 4R00194, R00177, R01291 (3 reactions) Entire module: 4 (67%)R00194, R00177, R01291 (3 reactions) Blocked rxns: 0 Essential rxns: 3R00194, R00177, R01291	Annotated: 8 (80%)R04035, R01071, R04640, R03013, R04037, R03457, R04558, R03243 (8 reactions) Annot. missing: 2 (20%)R03012, R01163 (2 reactions) Unpredicted: 0 Entire module: 8 (80%)R04035, R01071, R04640, R03013, R04037, R03457, R04558, R03243 (8 reactions) Blocked rxns: 0 Essential rxns: 8R04035, R01071, R04640, R03013, R04037, R03457, R04558, R03243	Annotated: 7 (78%)R02291, R02735, R00451, R04365, R02734, R04475, R00480 (7 reactions) Annot. missing: 2 (22%)R04198, R04199, R10147 (3 reactions) Unpredicted: 0 Entire module: 7 (78%)R02291, R02735, R00451, R04365, R02734, R04475, R00480 (7 reactions) Blocked rxns: 0 Essential rxns: 7R02291, R02735, R00451, R04365, R02734, R04475, R00480	Annotated: 3 (100%)R00713, R00714, R00261, R01648 (4 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)R00713, R00714, R00261, R01648 (4 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)R00497, R00894 (2 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)R00497, R00894 (2 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)R00670 (1 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (50%)R00670 (1 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (100%)R00178, R00566, R01157, R01920 (4 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)R00178, R00566, R01157, R01920 (4 reactions) Blocked rxns: 2R00178, R01920 Essential rxns: 0	Annotated: 4 (100%)R07415, R07419, R07414, R07418 (4 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)R07415, R07419, R07414, R07418 (4 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (100%)R02291, R01771, R01466, R00480, R01775 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)R01771, R02291, R01466, R00480, R01775 (5 reactions) Blocked rxns: 0 Essential rxns: 5R02291, R01771, R01466, R00480, R01775	Annotated: 1 (50%)R02565, R02566 (2 reactions) Annot. missing: 1 (50%)R01025 (1 reactions) Unpredicted: 0 Entire module: 1 (50%)R02565, R02566 (2 reactions) Blocked rxns: 2R02565, R02566 Essential rxns: 0	Annotated: 3 (100%)R01513, R04173, R00582 (3 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)R01513, R04173, R00582 (3 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)R00480, R02291 (2 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (40%)R00480, R02291 (2 reactions) Blocked rxns: 0 Essential rxns: 2R00480, R02291

	Glycan metabolism
	Lipopolysaccharide metabolism
	ADP-L-glycero-D-manno-heptose biosynthesis	KDO2-lipid A biosynthesis, Raetz pathway, LpxL-LpxM type	CMP-KDO biosynthesis
e_coli_core	Annotated: 0 Annot. missing: 5 (100%)R05644, R05176, R05646, R05645, R05647 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 10 (100%)R04587, R04550, R04549, R05074, R04657, R05075, R04567, R04606, R05146, R04658 (10 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 4 (100%)R03350, R01530, R03351, R03254 (4 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 4 (80%)R05644, R05176, R05645, R05647 (4 reactions) Annot. missing: 1 (20%)R05646 (1 reactions) Unpredicted: 0 Entire module: 4 (80%)R05644, R05176, R05645, R05647 (4 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 10 (100%)R04587, R04550, R04549, R05074, R04657, R05075, R04567, R04606, R05146, R04658 (10 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 10 (100%)R04587, R04550, R04549, R04657, R05075, R04567, R05074, R05146, R04658, R04606 (10 reactions) Blocked rxns: 0 Essential rxns: 8R04587, R04550, R04549, R05074, R04657, R04567, R04606, R04658	Annotated: 2 (50%)R01530, R03254 (2 reactions) Annot. missing: 2 (50%)R03350, R03351 (2 reactions) Unpredicted: 0 Entire module: 2 (50%)R01530, R03254 (2 reactions) Blocked rxns: 0 Essential rxns: 2R01530, R03254

	Metabolism of cofactors and vitamins
	Cofactor and vitamin metabolism
	Pyridoxal-P biosynthesis, erythrose-4P => pyridoxal-P	Pimeloyl-ACP biosynthesis, BioC-BioH pathway, malonyl-ACP => pimeloyl-ACP	Pantothenate biosynthesis, valine/L-aspartate => pantothenate	Biotin biosynthesis, pimeloyl-ACP/CoA => biotin	Cobalamin biosynthesis, cobyrinate a,c-diamide => cobalamin	Riboflavin biosynthesis, plants and bacteria, GTP => riboflavin/FMN/FAD	Tetrahydrofolate biosynthesis, GTP => THF	Ubiquinone biosynthesis, prokaryotes, chorismate (+ polyprenyl-PP) => ubiquinol	C1-unit interconversion, prokaryotes	Siroheme biosynthesis, glutamyl-tRNA => siroheme	Molybdenum cofactor biosynthesis, GTP => molybdenum cofactor	Heme biosynthesis, plants and bacteria, glutamate => heme	Menaquinone biosynthesis, chorismate (+ polyprenyl-PP) => menaquinol	NAD biosynthesis, aspartate => quinolinate => NAD	Thiamine biosynthesis, prokaryotes, AIR (+ DXP/tyrosine) => TMP/TPP	Coenzyme A biosynthesis, pantothenate => CoA
e_coli_core	Annotated: 0 Annot. missing: 7 (100%)R05085, R05681, R05838, R07406, R00278, R04210, R01825 (7 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 10 (100%)R09725, R10119, R09543, R10116, R10122, R10115, R10117, R10120, R10118, R10121 (10 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 5 (100%)R01214, R02473, R00489, R02472, R01226 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 5 (100%)R10124, R03210, R01078, R03182, R03231 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 6 (100%)R12184, R05222, R05223, R04148, R06558, R04594, R05221 (7 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 9 (100%)R00549, R04457, R07281, R00066, R00161, R03458, R00425, R03459, R07280 (9 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 12 (100%)R00936, R05046, R04638, R03067, R05048, R03504, R00428, R03066, R02237, R03503, R00939, R04620, R04639 (13 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 9 (100%)R04985, R12657, R09737, R12658, R08768, R08769, R12067, R01302, R05000 (9 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R00945, R01220, R01655 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 8 (100%)R00036, R03194, R04109, R03165, R03947, R00084, R02864, R02272 (8 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 5 (100%)R09394, R09726, R09395, R09735, R11372 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 10 (100%)R00036, R03220, R04109, R00310, R03165, R03197, R00084, R02272, R05578, R06895, R09489 (11 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 9 (100%)R08165, R04031, R01717, R07263, R09736, R08166, R10757, R07262, R04030 (9 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 5 (100%)R00189, R04292, R03348, R03005, R00481 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 8 (100%)R04509, R10246, R00617, R03472, R10247, R07461, R10712, R07459 (8 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 5 (100%)R04231, R03035, R03269, R00130, R03018 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 4 (57%)R01825, R05085, R04210, R00278 (4 reactions) Annot. missing: 3 (43%)R07406, R05681, R05838 (3 reactions) Unpredicted: 0 Entire module: 4 (57%)R01825, R05085, R04210, R00278 (4 reactions) Blocked rxns: 0 Essential rxns: 3R01825, R05085, R04210	Annotated: 0 Annot. missing: 10 (100%)R09725, R10119, R09543, R10116, R10122, R10115, R10117, R10120, R10118, R10121 (10 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (100%)R01214, R02473, R00489, R02472, R01226 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)R01214, R02473, R00489, R02472, R01226 (5 reactions) Blocked rxns: 0 Essential rxns: 4R02472, R02473, R01226, R00489	Annotated: 4 (80%)R03182, R03210, R01078, R03231 (4 reactions) Annot. missing: 1 (20%)R10124 (1 reactions) Unpredicted: 0 Entire module: 4 (67%)R03182, R03210, R01078, R03231 (4 reactions) Blocked rxns: 4R03182, R03210, R01078, R03231 Essential rxns: 0	Annotated: 5 (83%)R05222, R05223, R04148, R04594, R05221 (5 reactions) Annot. missing: 1 (17%)R12184 (1 reactions) Unpredicted: 0 Entire module: 5 (56%)R05222, R05223, R04148, R04594, R05221 (5 reactions) Blocked rxns: 5R05222, R05223, R04148, R04594, R05221 Essential rxns: 0	Annotated: 9 (100%)R00549, R04457, R07281, R00066, R00161, R03458, R00425, R03459, R07280 (9 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 9 (100%)R00549, R04457, R07281, R00161, R00066, R03458, R00425, R03459, R07280 (9 reactions) Blocked rxns: 0 Essential rxns: 9R00549, R04457, R07281, R00066, R00161, R03458, R00425, R03459, R07280	Annotated: 6 (50%)R04638, R03067, R03504, R00939, R02237, R03503 (6 reactions) Annot. missing: 6 (50%)R05046, R05048, R00428, R03066, R04620, R04639 (6 reactions) Unpredicted: 1 Entire module: 7 (54%)R04638, R03067, R03504, R00939, R03503, R02237 (6 reactions) Blocked rxns: 0 Essential rxns: 6R04638, R03067, R03504, R00939, R02237, R03503	Annotated: 1 (11%)R01302 (1 reactions) Annot. missing: 8 (89%)R04985, R12657, R09737, R12658, R08768, R08769, R12067, R05000 (8 reactions) Unpredicted: 0 Entire module: 1 (11%)R01302 (1 reactions) Blocked rxns: 0 Essential rxns: 1R01302	Annotated: 3 (100%)R00945, R01220, R01655 (3 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (75%)R00945, R01220, R01655 (3 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 8 (100%)R00036, R03194, R04109, R03165, R03947, R00084, R02864, R02272 (8 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 8 (100%)R00036, R03194, R04109, R03165, R00084, R03947, R02864, R02272 (8 reactions) Blocked rxns: 0 Essential rxns: 8R00036, R03194, R04109, R03165, R03947, R00084, R02864, R02272	Annotated: 0 Annot. missing: 5 (100%)R09394, R09726, R09395, R09735, R11372 (5 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 10 (100%)R00036, R03220, R04109, R00310, R03165, R03197, R00084, R02272, R05578, R06895, R09489 (11 reactions) Annot. missing: 0 (0%) Unpredicted: 0R12605, R03222 (2 reactions) Entire module: 10 (100%)R00036, R03220, R04109, R03222, R00310, R00084, R03197, R03165, R02272, R05578, R12605, R06895, R09489 (13 reactions) Blocked rxns: 0 Essential rxns: 8R00036, R04109, R00310, R03165, R03197, R00084, R05578, R02272	Annotated: 3 (33%)R04031, R01717, R04030 (3 reactions) Annot. missing: 6 (67%)R08165, R07263, R09736, R08166, R10757, R07262 (6 reactions) Unpredicted: 0 Entire module: 3 (33%)R04031, R01717, R04030 (3 reactions) Blocked rxns: 2R04031, R04030 Essential rxns: 0	Annotated: 5 (100%)R00189, R04292, R03348, R03005, R00481 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)R00189, R04292, R03348, R03005, R00481 (5 reactions) Blocked rxns: 0 Essential rxns: 4R00189, R03005, R04292, R03348	Annotated: 2 (25%)R00617, R04509 (2 reactions) Annot. missing: 6 (75%)R10246, R03472, R10247, R07461, R10712, R07459 (6 reactions) Unpredicted: 0 Entire module: 2 (22%)R00617, R04509 (2 reactions) Blocked rxns: 0 Essential rxns: 2R00617, R04509	Annotated: 5 (100%)R04231, R03035, R03269, R00130, R03018 (5 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)R04231, R03035, R03269, R00130, R03018 (5 reactions) Blocked rxns: 0 Essential rxns: 5R04231, R03035, R03269, R00130, R03018

	Biosynthesis of terpenoids and polyketides
	Polyketide sugar unit biosynthesis	Terpenoid backbone biosynthesis		Type II polyketide biosynthesis
	dTDP-L-rhamnose biosynthesis	C5 isoprenoid biosynthesis, non-mevalonate pathway	C10-C20 isoprenoid biosynthesis, bacteria	Dihydrokalafungin biosynthesis, octaketide => dihydrokalafungin
e_coli_core	Annotated: 0 Annot. missing: 4 (100%)R06514, R02328, R06513, R02777 (4 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 8 (100%)R05884, R05634, R08689, R05636, R05637, R05633, R10859, R05688, R01123 (9 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 Annot. missing: 3 (100%)R01658, R02003, R01123 (3 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 4 (100%)R06514, R02328, R06513, R02777 (4 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)R06514, R02328, R06513, R02777 (4 reactions) Blocked rxns: 0 Essential rxns: 0	Annotated: 8 (100%)R05884, R05634, R08689, R05636, R05637, R05633, R10859, R05688, R01123 (9 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 8 (100%)R05884, R05634, R08689, R05636, R05637, R05633, R01123, R10859, R05688 (9 reactions) Blocked rxns: 0 Essential rxns: 7R05634, R08689, R05636, R05637, R05633, R10859, R05688	Annotated: 3 (100%)R01658, R02003, R01123 (3 reactions) Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (75%)R01658, R02003, R01123 (3 reactions) Blocked rxns: 0 Essential rxns: 2R01658, R02003	Unpredicted: 0R05705 (1 reactions) (R05705 is present but can not be highlighted on KEGG map) Entire module: 0 (0%)R05705 (1 reactions) Blocked rxns: 0 Essential rxns: 0

	Xenobiotics biodegradation
	Aromatics degradation
	Phenylacetate degradation, phenylaxetate => acetyl-CoA/succinyl-CoA
e_coli_core	Annotated: 0 Annot. missing: 9 (100%)R06942, R09838, R09836, R06941, R09837, R09820, R02539, R09839, R00829 (9 reactions) Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 1 (11%)R02539 (1 reactions) Annot. missing: 8 (89%)R06942, R09838, R09836, R06941, R09837, R09820, R09839, R00829 (8 reactions) Unpredicted: 0 Entire module: 1 (11%)R02539 (1 reactions) Blocked rxns: 1R02539 Essential rxns: 0

Are shown only KEGG Modules with four or more processing steps and at least 50% completion.

The table below shows how reactions in the model maps with reactions on BioCyc Pathways. The result is divided into six categories for each module:
Organism expected: The number of reactions in the database present in the model (completness of pathway). With mouse over: the reactions in the model mapped to BioCyc.
Organism missing: The number of reactions in the database not present in the model. With mouse over: the reactions present in the model mapped to BioCyc.
Unpredicted: for some organisms, the reference database expects only part of a module to be present. This category receives the number of reactions present in the module, but not expected according to the database.
Entire module: Sum of 'Organism expected' and 'Unpredicted'.
Blocked: number of blocked reactions in pathway.
Essentials: number of essential reactions in pathway.
By clicking on the table's links, the BioCyc reactions and metabolites present in the model are highlighted in a BioCyc Module map.
Units used in the table: The entry "Annotated: 2 (50%)" can be read as "2 reactions (50% pathway completeness)".
Regarding reactions mapping: For mapping model reactions against BioCyc reactions, two sources are used: (i) checking for information on BioCyc reaction synonyms stored in the SBML model, and (ii) the MNXref database. Synonyms found in (i) and (ii) are consolidated.
Color code:
Green ▶: Complete pathways, or pathways where all reactions predicted by the database are present in the model.
Yellow ▶: Incomplete pathways, but with at least one reaction present in the model.
Pink ▶: Incomplete pathways, with no reactions present in the model.
Blue ▶: Pathways not present in the selected organism, but MinPath suggested its presence.
White: Pathways suggested to exist in another model.

	N¹⁰-formyl-tetrahydrofolate biosynthesis	phenylethylamine degradation I	superpathway of 4-aminobutanoate degradation	superpathway of acetate utilization and formation	acetoacetate degradation (to acetyl CoA)	L-alanine degradation I	L-alanine biosynthesis II	L-alanine biosynthesis I	two-component alkanesulfonate monooxygenase	superpathway of chorismate metabolism
e_coli_core	Annotated: 0 (0%) Annot. missing: 9 (100%)METHENYLTHFCYCLOHYDRO-RXN, METHYLENETHFDEHYDROG-NADP-RXN, THYMIDYLATESYN-RXN, GLYOHMETRANS-RXN, HOMOCYSMETB12-RXN, GCVMULTI-RXN, 5-FORMYL-THF-CYCLO-LIGASE-RXN, DIHYDROFOLATEREDUCT-RXN, 1.5.1.20-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)PHENDEHYD-RXN, AMINEPHEN-RXN, TRANS-RXN0-284 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCSEMIALDDEHYDROG-RXN, GABATRANSAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (67%)PHOSACETYLTRANS-RXN, ACETATEKIN-RXN Annot. missing: 1 (33%)ACETATE--COA-LIGASE-RXN Unpredicted: 0 Entire module: 2 (67%)PHOSACETYLTRANS-RXN, ACETATEKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)ACETOACETYL-COA-TRANSFER-RXN, ACETYL-COA-ACETYLTRANSFER-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)ALARACECAT-RXN, DALADEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ALANINE-AMINOTRANSFERASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)ALARACECAT-RXN, BRANCHED-CHAINAMINOTRANSFERVAL-RXN, VALINE-PYRUVATE-AMINOTRANSFER-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-280, RXN-12444 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 60 (100%)PRTRANS-RXN, 3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, TYROSINE-AMINOTRANSFERASE-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, PABASYN-RXN, O-SUCCINYLBENZOATE-COA-SYN-RXN, 2-OCTAPRENYLPHENOL-HYDROX-RXN, PREPHENATEDEHYDROG-RXN, RXN-9310, RXN-15889, 2.5.1.64-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, PREPHENATEDEHYDRAT-RXN, 4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, ENTG-RXN, NAPHTHOATE-SYN-RXN, CHORPYRLY-RXN, RXN-8992, H2PTERIDINEPYROPHOSPHOKIN-RXN, RXN-9311, O-SUCCINYLBENZOATE-COA-LIG-RXN, 2-OCTAPRENYL-6-OHPHENOL-METHY-RXN, RXN-10814, ISOCHORMAT-RXN, GPPSYN-RXN, DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, PRAISOM-RXN, ISOCHORSYN-RXN, ADCLY-RXN, OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-RXN, ANTHRANSYN-RXN, H2NEOPTERINALDOL-RXN, ENTF-RXN, SHIKIMATE-KINASE-RXN, DIHYDROFOLATESYNTH-RXN, 2-OCTAPRENYL-6-METHOXYPHENOL-HYDROX-RXN, DIHYDROFOLATEREDUCT-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, IPPISOM-RXN, ENTDB-RXN, IGPSYN-RXN, DHBAMPLIG-RXN, DMK-RXN, CHORISMATEMUT-RXN, DHBDEHYD-RXN, H2PTEROATESYNTH-RXN, FPPSYN-RXN, DAHPSYN-RXN, RXN0-2382, RXN0-2381, RXN-15891, RXN-15890, RXN0-6442, GTP-CYCLOHYDRO-I-RXN, ADOMET-DMK-METHYLTRANSFER-RXN, DHHB-METHYLTRANSFER-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 0 (0%) Annot. missing: 9 (100%)METHENYLTHFCYCLOHYDRO-RXN, METHYLENETHFDEHYDROG-NADP-RXN, THYMIDYLATESYN-RXN, GLYOHMETRANS-RXN, HOMOCYSMETB12-RXN, GCVMULTI-RXN, 5-FORMYL-THF-CYCLO-LIGASE-RXN, DIHYDROFOLATEREDUCT-RXN, 1.5.1.20-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (67%)PHENDEHYD-RXN, AMINEPHEN-RXN Annot. missing: 1 (33%)TRANS-RXN0-284 Unpredicted: 0 Entire module: 2 (100%)PHENDEHYD-RXN, AMINEPHEN-RXN Blocked rxns: 1PHENDEHYD-RXN Essential rxns: 0	Annotated: 3 (100%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCSEMIALDDEHYDROG-RXN, GABATRANSAM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCSEMIALDDEHYDROG-RXN, GABATRANSAM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)PHOSACETYLTRANS-RXN, ACETATE--COA-LIGASE-RXN, ACETATEKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)PHOSACETYLTRANS-RXN, ACETATE--COA-LIGASE-RXN, ACETATEKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)ACETOACETYL-COA-TRANSFER-RXN, ACETYL-COA-ACETYLTRANSFER-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)ACETOACETYL-COA-TRANSFER-RXN, ACETYL-COA-ACETYLTRANSFER-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (50%)ALARACECAT-RXN Annot. missing: 1 (50%)DALADEHYDROG-RXN Unpredicted: 0 Entire module: 1 (50%)ALARACECAT-RXN Blocked rxns: 0 Essential rxns: 1ALARACECAT-RXN	Annotated: 1 (100%)ALANINE-AMINOTRANSFERASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ALANINE-AMINOTRANSFERASE-RXN Blocked rxns: 0 Essential rxns: 1ALANINE-AMINOTRANSFERASE-RXN	Annotated: 3 (100%)ALARACECAT-RXN, BRANCHED-CHAINAMINOTRANSFERVAL-RXN, VALINE-PYRUVATE-AMINOTRANSFER-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)ALARACECAT-RXN, BRANCHED-CHAINAMINOTRANSFERVAL-RXN, VALINE-PYRUVATE-AMINOTRANSFER-RXN Blocked rxns: 0 Essential rxns: 1ALARACECAT-RXN	Annotated: 1 (50%)RXN-12444 Annot. missing: 1 (50%)RXN0-280 Unpredicted: 0 Entire module: 1 (50%)RXN-12444 Blocked rxns: 0 Essential rxns: 0	Annotated: 46 (77%)PRTRANS-RXN, 3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, TYROSINE-AMINOTRANSFERASE-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, PABASYN-RXN, O-SUCCINYLBENZOATE-COA-SYN-RXN, PREPHENATEDEHYDROG-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, PREPHENATEDEHYDRAT-RXN, 4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, CHORPYRLY-RXN, RXN-8992, H2PTERIDINEPYROPHOSPHOKIN-RXN, O-SUCCINYLBENZOATE-COA-LIG-RXN, 2-OCTAPRENYL-6-OHPHENOL-METHY-RXN, RXN-10814, ISOCHORMAT-RXN, GPPSYN-RXN, DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, PRAISOM-RXN, ISOCHORSYN-RXN, ADCLY-RXN, ANTHRANSYN-RXN, H2NEOPTERINALDOL-RXN, ENTF-RXN, SHIKIMATE-KINASE-RXN, DIHYDROFOLATESYNTH-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, IPPISOM-RXN, IGPSYN-RXN, DHBAMPLIG-RXN, DMK-RXN, CHORISMATEMUT-RXN, DHBDEHYD-RXN, H2PTEROATESYNTH-RXN, FPPSYN-RXN, DAHPSYN-RXN, RXN0-2382, RXN0-2381, GTP-CYCLOHYDRO-I-RXN, ADOMET-DMK-METHYLTRANSFER-RXN, DHHB-METHYLTRANSFER-RXN Annot. missing: 14 (23%)OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-RXN, ENTG-RXN, 2-OCTAPRENYLPHENOL-HYDROX-RXN, RXN-15891, NAPHTHOATE-SYN-RXN, RXN-9310, RXN-15889, RXN-15890, RXN-9311, 2.5.1.64-RXN, 2-OCTAPRENYL-6-METHOXYPHENOL-HYDROX-RXN, DIHYDROFOLATEREDUCT-RXN, RXN0-6442, ENTDB-RXN Unpredicted: 0 Entire module: 46 (77%)PRTRANS-RXN, 3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, TYROSINE-AMINOTRANSFERASE-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, PABASYN-RXN, O-SUCCINYLBENZOATE-COA-SYN-RXN, PREPHENATEDEHYDROG-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, PREPHENATEDEHYDRAT-RXN, 4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, CHORPYRLY-RXN, RXN-8992, H2PTERIDINEPYROPHOSPHOKIN-RXN, O-SUCCINYLBENZOATE-COA-LIG-RXN, 2-OCTAPRENYL-6-OHPHENOL-METHY-RXN, RXN-10814, ISOCHORMAT-RXN, GPPSYN-RXN, DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, PRAISOM-RXN, ISOCHORSYN-RXN, ADCLY-RXN, ANTHRANSYN-RXN, H2NEOPTERINALDOL-RXN, ENTF-RXN, SHIKIMATE-KINASE-RXN, DIHYDROFOLATESYNTH-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, IPPISOM-RXN, IGPSYN-RXN, DHBAMPLIG-RXN, DMK-RXN, CHORISMATEMUT-RXN, DHBDEHYD-RXN, H2PTEROATESYNTH-RXN, FPPSYN-RXN, DAHPSYN-RXN, RXN0-2382, RXN0-2381, GTP-CYCLOHYDRO-I-RXN, ADOMET-DMK-METHYLTRANSFER-RXN, DHHB-METHYLTRANSFER-RXN Blocked rxns: 7O-SUCCINYLBENZOATE-COA-SYN-RXN, O-SUCCINYLBENZOATE-COA-LIG-RXN, 2-OCTAPRENYL-6-OHPHENOL-METHY-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, DHHB-METHYLTRANSFER-RXN, ADOMET-DMK-METHYLTRANSFER-RXN, DMK-RXN Essential rxns: 31PRTRANS-RXN, 3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, TYROSINE-AMINOTRANSFERASE-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, PABASYN-RXN, PREPHENATEDEHYDROG-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, PREPHENATEDEHYDRAT-RXN, 4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, CHORPYRLY-RXN, RXN-8992, H2PTERIDINEPYROPHOSPHOKIN-RXN, RXN-10814, GPPSYN-RXN, DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, PRAISOM-RXN, ADCLY-RXN, H2NEOPTERINALDOL-RXN, SHIKIMATE-KINASE-RXN, DIHYDROFOLATESYNTH-RXN, IGPSYN-RXN, CHORISMATEMUT-RXN, H2PTEROATESYNTH-RXN, FPPSYN-RXN, DAHPSYN-RXN, GTP-CYCLOHYDRO-I-RXN, ANTHRANSYN-RXN

	ammonia assimilation cycle III	L-arabinose degradation I	superpathway of arginine and polyamine biosynthesis	superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	L-arginine biosynthesis I (via L-ornithine)	chorismate biosynthesis I	L-asparagine biosynthesis I	L-asparagine degradation I	L-asparagine biosynthesis II	L-aspartate biosynthesis
e_coli_core	Annotated: 2 (100%)GLUTAMATESYN-RXN, GLUTAMINESYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)GLUTAMATESYN-RXN, GLUTAMINESYN-RXN Blocked rxns: 0 Essential rxns: 1GLUTAMINESYN-RXN	Annotated: 0 (0%) Annot. missing: 3 (100%)ARABISOM-RXN, RIBULPEPIM-RXN, RXN0-5116 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 16 (100%)CARBPSYN-RXN, ORNCARBAMTRANSFER-RXN, LYSDECARBOX-RXN, N-ACETYLTRANSFER-RXN, ACETYLORNTRANSAM-RXN, ARGSUCCINLYA-RXN, N-ACETYLGLUTPREDUCT-RXN, ARGSUCCINSYN-RXN, ARGDECARBOX-RXN, AGMATIN-RXN, RXN0-5217, ACETYLORNDEACET-RXN, ORNDECARBOX-RXN, SPERMIDINESYN-RXN, SAMDECARB-RXN, ACETYLGLUTKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 11 (100%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCSEMIALDDEHYDROG-RXN, AMINOBUTDEHYDROG-RXN, AGMATIN-RXN, RXN0-3901, ARGDECARBOX-RXN, RXN0-3921, RXN0-3942, RXN0-3922, GABATRANSAM-RXN, PUTTRANSAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 9 (100%)CARBPSYN-RXN, ORNCARBAMTRANSFER-RXN, N-ACETYLTRANSFER-RXN, ACETYLORNTRANSAM-RXN, ARGSUCCINLYA-RXN, N-ACETYLGLUTPREDUCT-RXN, ARGSUCCINSYN-RXN, ACETYLORNDEACET-RXN, ACETYLGLUTKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, DAHPSYN-RXN, SHIKIMATE-KINASE-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ASNSYNB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ASPARAGHYD-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ASNSYNA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ASPAMINOTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 2 (100%)GLUTAMATESYN-RXN, GLUTAMINESYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)GLUTAMATESYN-RXN, GLUTAMINESYN-RXN Blocked rxns: 0 Essential rxns: 1GLUTAMINESYN-RXN	Annotated: 2 (67%)RXN0-5116, RIBULPEPIM-RXN Annot. missing: 1 (33%)ARABISOM-RXN Unpredicted: 0 Entire module: 2 (67%)RXN0-5116, RIBULPEPIM-RXN Blocked rxns: 1RXN0-5116 Essential rxns: 0	Annotated: 15 (94%)CARBPSYN-RXN, ORNCARBAMTRANSFER-RXN, LYSDECARBOX-RXN, N-ACETYLTRANSFER-RXN, ACETYLORNTRANSAM-RXN, ARGSUCCINLYA-RXN, N-ACETYLGLUTPREDUCT-RXN, ARGSUCCINSYN-RXN, ARGDECARBOX-RXN, AGMATIN-RXN, ACETYLORNDEACET-RXN, ORNDECARBOX-RXN, SPERMIDINESYN-RXN, SAMDECARB-RXN, ACETYLGLUTKIN-RXN Annot. missing: 1 (6%)RXN0-5217 Unpredicted: 0 Entire module: 15 (94%)CARBPSYN-RXN, ORNCARBAMTRANSFER-RXN, LYSDECARBOX-RXN, N-ACETYLTRANSFER-RXN, ACETYLORNTRANSAM-RXN, ARGSUCCINLYA-RXN, N-ACETYLGLUTPREDUCT-RXN, ARGSUCCINSYN-RXN, ARGDECARBOX-RXN, AGMATIN-RXN, ACETYLORNDEACET-RXN, ORNDECARBOX-RXN, SPERMIDINESYN-RXN, SAMDECARB-RXN, ACETYLGLUTKIN-RXN Blocked rxns: 2SPERMIDINESYN-RXN, SAMDECARB-RXN Essential rxns: 8ORNCARBAMTRANSFER-RXN, N-ACETYLTRANSFER-RXN, ARGSUCCINLYA-RXN, ACETYLORNTRANSAM-RXN, N-ACETYLGLUTPREDUCT-RXN, ARGSUCCINSYN-RXN, ACETYLORNDEACET-RXN, ACETYLGLUTKIN-RXN	Annotated: 8 (73%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCSEMIALDDEHYDROG-RXN, ARGDECARBOX-RXN, AMINOBUTDEHYDROG-RXN, AGMATIN-RXN, RXN0-3942, GABATRANSAM-RXN, PUTTRANSAM-RXN Annot. missing: 3 (27%)RXN0-3901, RXN0-3922, RXN0-3921 Unpredicted: 0 Entire module: 8 (73%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCSEMIALDDEHYDROG-RXN, ARGDECARBOX-RXN, AMINOBUTDEHYDROG-RXN, AGMATIN-RXN, RXN0-3942, GABATRANSAM-RXN, PUTTRANSAM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 9 (100%)CARBPSYN-RXN, ORNCARBAMTRANSFER-RXN, N-ACETYLTRANSFER-RXN, ACETYLORNTRANSAM-RXN, ARGSUCCINLYA-RXN, N-ACETYLGLUTPREDUCT-RXN, ARGSUCCINSYN-RXN, ACETYLORNDEACET-RXN, ACETYLGLUTKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 9 (100%)CARBPSYN-RXN, ORNCARBAMTRANSFER-RXN, N-ACETYLTRANSFER-RXN, ACETYLORNTRANSAM-RXN, ARGSUCCINLYA-RXN, N-ACETYLGLUTPREDUCT-RXN, ARGSUCCINSYN-RXN, ACETYLORNDEACET-RXN, ACETYLGLUTKIN-RXN Blocked rxns: 0 Essential rxns: 8ORNCARBAMTRANSFER-RXN, N-ACETYLTRANSFER-RXN, ARGSUCCINLYA-RXN, ACETYLORNTRANSAM-RXN, N-ACETYLGLUTPREDUCT-RXN, ARGSUCCINSYN-RXN, ACETYLORNDEACET-RXN, ACETYLGLUTKIN-RXN	Annotated: 7 (100%)3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, DAHPSYN-RXN, SHIKIMATE-KINASE-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 7 (100%)3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, DAHPSYN-RXN, SHIKIMATE-KINASE-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN Blocked rxns: 0 Essential rxns: 73-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, DAHPSYN-RXN, SHIKIMATE-KINASE-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN	Annotated: 1 (100%)ASNSYNB-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ASNSYNB-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)ASPARAGHYD-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ASPARAGHYD-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)ASNSYNA-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ASNSYNA-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)ASPAMINOTRANS-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ASPAMINOTRANS-RXN Blocked rxns: 0 Essential rxns: 1ASPAMINOTRANS-RXN

	superpathway of L-aspartate and L-asparagine biosynthesis	L-arginine degradation II (AST pathway)	glycine betaine biosynthesis I (Gram-negative bacteria)	lactose degradation III	biotin biosynthesis I	superpathway of branched chain amino acid biosynthesis	spermidine biosynthesis I	L-carnitine degradation I	coenzyme A biosynthesis I (prokaryotic)	superpathway of adenosylcobalamin salvage from cobinamide I
e_coli_core	Annotated: 0 (0%) Annot. missing: 4 (100%)ASNSYNA-RXN, ASPARAGHYD-RXN, ASPARTASE-RXN, ASPAMINOTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)SUCCGLUALDDEHYD-RXN, SUCCORNTRANSAM-RXN, SUCCARGDIHYDRO-RXN, ARGININE-N-SUCCINYLTRANSFERASE-RXN, SUCCGLUDESUCC-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)BADH-RXN, CHD-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)BETAGALACTOSID-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 14 (100%)RXN-11482, RXN-11483, DAPASYN-RXN, RXN-11479, RXN-11475, RXN-11474, RXN-11481, RXN-11477, RXN-11476, 2.8.1.6-RXN, DETHIOBIOTIN-SYN-RXN, RXN-11478, RXN-11484, RXN-11480 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 17 (100%)BRANCHED-CHAINAMINOTRANSFERLEU-RXN, RXN-15122, RXN-15121, ACETOOHBUTREDUCTOISOM-RXN, RXN-8991, ACETOOHBUTSYN-RXN, DIHYDROXYMETVALDEHYDRAT-RXN, RXN-15123, BRANCHED-CHAINAMINOTRANSFERILEU-RXN, RXN-7800, BRANCHED-CHAINAMINOTRANSFERVAL-RXN, DIHYDROXYISOVALDEHYDRAT-RXN, 3-ISOPROPYLMALISOM-RXN, 2-ISOPROPYLMALATESYN-RXN, 3-ISOPROPYLMALDEHYDROG-RXN, ACETOLACTSYN-RXN, ACETOLACTREDUCTOISOM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)SPERMIDINESYN-RXN, SAMDECARB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN0-3601, CARNDETRU-RXN, CROBETREDUCT-RXN, LCARNCOALIG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)P-PANTOCYSLIG-RXN, DEPHOSPHOCOAKIN-RXN, P-PANTOCYSDECARB-RXN, PANTEPADENYLYLTRAN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)RXN-19343, COBINAMIDEKIN-RXN, RXN-8770, RXN-19338, COBALAMIN5PSYN-RXN, DMBPPRIBOSYLTRANS-RXN, RXN-19342, COBINPGUANYLYLTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 4 (100%)ASNSYNA-RXN, ASPARAGHYD-RXN, ASPARTASE-RXN, ASPAMINOTRANS-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)ASNSYNA-RXN, ASPARAGHYD-RXN, ASPARTASE-RXN, ASPAMINOTRANS-RXN Blocked rxns: 0 Essential rxns: 1ASPAMINOTRANS-RXN	Annotated: 5 (100%)SUCCGLUALDDEHYD-RXN, SUCCORNTRANSAM-RXN, SUCCARGDIHYDRO-RXN, ARGININE-N-SUCCINYLTRANSFERASE-RXN, SUCCGLUDESUCC-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)SUCCGLUALDDEHYD-RXN, SUCCORNTRANSAM-RXN, SUCCARGDIHYDRO-RXN, ARGININE-N-SUCCINYLTRANSFERASE-RXN, SUCCGLUDESUCC-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (50%)BADH-RXN Annot. missing: 1 (50%)CHD-RXN Unpredicted: 0 Entire module: 1 (50%)BADH-RXN Blocked rxns: 1BADH-RXN Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)BETAGALACTOSID-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (14%)DAPASYN-RXN, DETHIOBIOTIN-SYN-RXN Annot. missing: 12 (86%)RXN-11482, RXN-11483, RXN-11479, RXN-11475, RXN-11474, RXN-11481, RXN-11477, RXN-11476, 2.8.1.6-RXN, RXN-11478, RXN-11484, RXN-11480 Unpredicted: 0 Entire module: 2 (14%)DAPASYN-RXN, DETHIOBIOTIN-SYN-RXN Blocked rxns: 2DAPASYN-RXN, DETHIOBIOTIN-SYN-RXN Essential rxns: 0	Annotated: 14 (82%)BRANCHED-CHAINAMINOTRANSFERLEU-RXN, ACETOLACTSYN-RXN, ACETOOHBUTREDUCTOISOM-RXN, RXN-8991, DIHYDROXYMETVALDEHYDRAT-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN, RXN-7800, BRANCHED-CHAINAMINOTRANSFERVAL-RXN, DIHYDROXYISOVALDEHYDRAT-RXN, 3-ISOPROPYLMALISOM-RXN, 2-ISOPROPYLMALATESYN-RXN, 3-ISOPROPYLMALDEHYDROG-RXN, ACETOOHBUTSYN-RXN, ACETOLACTREDUCTOISOM-RXN Annot. missing: 3 (18%)RXN-15122, RXN-15123, RXN-15121 Unpredicted: 0 Entire module: 13 (81%)BRANCHED-CHAINAMINOTRANSFERLEU-RXN, ACETOLACTSYN-RXN, ACETOOHBUTREDUCTOISOM-RXN, RXN-8991, DIHYDROXYMETVALDEHYDRAT-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN, RXN-7800, BRANCHED-CHAINAMINOTRANSFERVAL-RXN, DIHYDROXYISOVALDEHYDRAT-RXN, 2-ISOPROPYLMALATESYN-RXN, 3-ISOPROPYLMALDEHYDROG-RXN, ACETOOHBUTSYN-RXN, ACETOLACTREDUCTOISOM-RXN Blocked rxns: 0 Essential rxns: 13BRANCHED-CHAINAMINOTRANSFERLEU-RXN, ACETOLACTSYN-RXN, ACETOOHBUTREDUCTOISOM-RXN, RXN-8991, DIHYDROXYMETVALDEHYDRAT-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN, RXN-7800, 3-ISOPROPYLMALISOM-RXN, DIHYDROXYISOVALDEHYDRAT-RXN, 2-ISOPROPYLMALATESYN-RXN, 3-ISOPROPYLMALDEHYDROG-RXN, ACETOOHBUTSYN-RXN, ACETOLACTREDUCTOISOM-RXN	Annotated: 2 (100%)SPERMIDINESYN-RXN, SAMDECARB-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)SPERMIDINESYN-RXN, SAMDECARB-RXN Blocked rxns: 2SPERMIDINESYN-RXN, SAMDECARB-RXN Essential rxns: 0	Annotated: 2 (50%)RXN0-3601, CARNDETRU-RXN Annot. missing: 2 (50%)CROBETREDUCT-RXN, LCARNCOALIG-RXN Unpredicted: 0 Entire module: 2 (50%)RXN0-3601, CARNDETRU-RXN Blocked rxns: 2RXN0-3601, CARNDETRU-RXN Essential rxns: 0	Annotated: 4 (100%)P-PANTOCYSLIG-RXN, DEPHOSPHOCOAKIN-RXN, P-PANTOCYSDECARB-RXN, PANTEPADENYLYLTRAN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)P-PANTOCYSLIG-RXN, DEPHOSPHOCOAKIN-RXN, P-PANTOCYSDECARB-RXN, PANTEPADENYLYLTRAN-RXN Blocked rxns: 0 Essential rxns: 4P-PANTOCYSLIG-RXN, DEPHOSPHOCOAKIN-RXN, P-PANTOCYSDECARB-RXN, PANTEPADENYLYLTRAN-RXN	Annotated: 3 (38%)DMBPPRIBOSYLTRANS-RXN, COBINAMIDEKIN-RXN, COBINPGUANYLYLTRANS-RXN Annot. missing: 5 (62%)RXN-19343, RXN-8770, RXN-19338, COBALAMIN5PSYN-RXN, RXN-19342 Unpredicted: 0 Entire module: 3 (38%)DMBPPRIBOSYLTRANS-RXN, COBINAMIDEKIN-RXN, COBINPGUANYLYLTRANS-RXN Blocked rxns: 3DMBPPRIBOSYLTRANS-RXN, COBINAMIDEKIN-RXN, COBINPGUANYLYLTRANS-RXN Essential rxns: 0

	colanic acid building blocks biosynthesis	superpathway of aromatic amino acid biosynthesis	cyanate degradation	L-cysteine biosynthesis I	L-lysine biosynthesis I	D-arabinose degradation I	superpathway of purine nucleotides de novo biosynthesis II	superoxide radicals degradation	dTDP-L-rhamnose biosynthesis I	enterobacterial common antigen biosynthesis
e_coli_core	Annotated: 1 (9%)PGLUCISOM-RXN Annot. missing: 10 (91%)GLUC1PURIDYLTRANS-RXN, FCLREDUCT-RXN, UDPGLUCEPIM-RXN, UGD-RXN, PHOSPHOGLUCMUT-RXN, PHOSMANMUT-RXN, GDPMANDEHYDRA-RXN, 2.7.7.13-RXN, FCLEPIM-RXN, MANNPISOM-RXN Unpredicted: 0 Entire module: 1 (9%)PGLUCISOM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 18 (100%)CHORISMATEMUT-RXN, PRTRANS-RXN, 3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, PRAISOM-RXN, RXN0-2382, DAHPSYN-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, TYROSINE-AMINOTRANSFERASE-RXN, RXN0-2381, SHIKIMATE-KINASE-RXN, PREPHENATEDEHYDROG-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, PREPHENATEDEHYDRAT-RXN, CHORISMATE-SYNTHASE-RXN, IGPSYN-RXN, ANTHRANSYN-RXN, RXN-10814 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-5222, R524-RXN, RXN0-5224 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)SERINE-O-ACETTRAN-RXN, ACSERLY-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 9 (100%)ASPARTATEKIN-RXN, TETHYDPICSUCC-RXN, DIAMINOPIMDECARB-RXN, DIAMINOPIMEPIM-RXN, SUCCDIAMINOPIMDESUCC-RXN, RXN-14014, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCINYLDIAMINOPIMTRANS-RXN, DIHYDRODIPICSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)DARABISOM-RXN, GLYCOLALD-DEHYDROG-RXN, DARABALDOL-RXN, DARABKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (4%)ADENYL-KIN-RXN Annot. missing: 25 (96%)GDPKIN-RXN, GUANYL-KIN-RXN, RXN0-743, PRPPAMIDOTRANS-RXN, GMP-SYN-GLUT-RXN, GARTRANSFORMYL2-RXN, AICARTRANSFORM-RXN, RXN0-745, ADENYLOSUCCINATE-SYNTHASE-RXN, AICARSYN-RXN, RXN0-747, DADPKIN-RXN, ADPREDUCT-RXN, IMP-DEHYDROG-RXN, RXN0-748, RXN0-742, RXN0-746, DGDPKIN-RXN, GDPREDUCT-RXN, AMPSYN-RXN, FGAMSYN-RXN, SAICARSYN-RXN, IMPCYCLOHYDROLASE-RXN, GLYRIBONUCSYN-RXN, AIRS-RXN Unpredicted: 0 Entire module: 1 (4%)ADENYL-KIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)CATAL-RXN, SUPEROX-DISMUT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)DTDPDEHYDRHAMEPIM-RXN, DTDPGLUCOSEPP-RXN, DTDPGLUCDEHYDRAT-RXN, DTDPDEHYRHAMREDUCT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 10 (100%)DTDPGLUCOSEPP-RXN, TDPFUCACTRANS-RXN, TRANS-RXN0-279, UDPGLCNACEPIM-RXN, RFFTRANS-RXN, GLCNACPTRANS-RXN, UDPMANACATRANS-RXN, UDPMANNACADEHYDROG-RXN, FUC4NACTRANS-RXN, DTDPGLUCDEHYDRAT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 8 (73%)GLUC1PURIDYLTRANS-RXN, UDPGLUCEPIM-RXN, UGD-RXN, PHOSPHOGLUCMUT-RXN, PGLUCISOM-RXN, PHOSMANMUT-RXN, GDPMANDEHYDRA-RXN, MANNPISOM-RXN Annot. missing: 3 (27%)2.7.7.13-RXN, FCLEPIM-RXN, FCLREDUCT-RXN Unpredicted: 0 Entire module: 8 (73%)GLUC1PURIDYLTRANS-RXN, UDPGLUCEPIM-RXN, UGD-RXN, PHOSPHOGLUCMUT-RXN, PGLUCISOM-RXN, PHOSMANMUT-RXN, GDPMANDEHYDRA-RXN, MANNPISOM-RXN Blocked rxns: 1GDPMANDEHYDRA-RXN Essential rxns: 0	Annotated: 18 (100%)CHORISMATEMUT-RXN, PRTRANS-RXN, 3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, PRAISOM-RXN, RXN0-2382, DAHPSYN-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, TYROSINE-AMINOTRANSFERASE-RXN, RXN0-2381, SHIKIMATE-KINASE-RXN, PREPHENATEDEHYDROG-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, PREPHENATEDEHYDRAT-RXN, CHORISMATE-SYNTHASE-RXN, IGPSYN-RXN, ANTHRANSYN-RXN, RXN-10814 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 18 (100%)CHORISMATEMUT-RXN, PRTRANS-RXN, 3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, PRAISOM-RXN, RXN0-2382, DAHPSYN-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, TYROSINE-AMINOTRANSFERASE-RXN, RXN0-2381, SHIKIMATE-KINASE-RXN, PREPHENATEDEHYDROG-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, PREPHENATEDEHYDRAT-RXN, CHORISMATE-SYNTHASE-RXN, IGPSYN-RXN, ANTHRANSYN-RXN, RXN-10814 Blocked rxns: 0 Essential rxns: 16CHORISMATEMUT-RXN, PRTRANS-RXN, 3-DEHYDROQUINATE-SYNTHASE-RXN, 2.5.1.19-RXN, PRAISOM-RXN, DAHPSYN-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, TYROSINE-AMINOTRANSFERASE-RXN, SHIKIMATE-KINASE-RXN, PREPHENATEDEHYDROG-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, PREPHENATEDEHYDRAT-RXN, CHORISMATE-SYNTHASE-RXN, IGPSYN-RXN, ANTHRANSYN-RXN, RXN-10814	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-5222, R524-RXN, RXN0-5224 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)SERINE-O-ACETTRAN-RXN, ACSERLY-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)SERINE-O-ACETTRAN-RXN, ACSERLY-RXN Blocked rxns: 0 Essential rxns: 2SERINE-O-ACETTRAN-RXN, ACSERLY-RXN	Annotated: 7 (78%)ASPARTATEKIN-RXN, TETHYDPICSUCC-RXN, DIAMINOPIMDECARB-RXN, DIAMINOPIMEPIM-RXN, SUCCDIAMINOPIMDESUCC-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCINYLDIAMINOPIMTRANS-RXN Annot. missing: 2 (22%)RXN-14014, DIHYDRODIPICSYN-RXN Unpredicted: 0 Entire module: 7 (78%)ASPARTATEKIN-RXN, TETHYDPICSUCC-RXN, DIAMINOPIMDECARB-RXN, DIAMINOPIMEPIM-RXN, SUCCDIAMINOPIMDESUCC-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCINYLDIAMINOPIMTRANS-RXN Blocked rxns: 0 Essential rxns: 7ASPARTATEKIN-RXN, TETHYDPICSUCC-RXN, DIAMINOPIMDECARB-RXN, DIAMINOPIMEPIM-RXN, SUCCDIAMINOPIMDESUCC-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCINYLDIAMINOPIMTRANS-RXN	Annotated: 1 (25%)GLYCOLALD-DEHYDROG-RXN Annot. missing: 3 (75%)DARABISOM-RXN, DARABALDOL-RXN, DARABKIN-RXN Unpredicted: 0 Entire module: 1 (25%)GLYCOLALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 1GLYCOLALD-DEHYDROG-RXN	Annotated: 19 (73%)GDPKIN-RXN, GUANYL-KIN-RXN, ADENYL-KIN-RXN, IMPCYCLOHYDROLASE-RXN, RXN0-743, GMP-SYN-GLUT-RXN, GARTRANSFORMYL2-RXN, ADENYLOSUCCINATE-SYNTHASE-RXN, AICARSYN-RXN, DADPKIN-RXN, ADPREDUCT-RXN, IMP-DEHYDROG-RXN, DGDPKIN-RXN, GDPREDUCT-RXN, AMPSYN-RXN, FGAMSYN-RXN, SAICARSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN Annot. missing: 7 (27%)AICARTRANSFORM-RXN, RXN0-748, RXN0-745, RXN0-742, RXN0-747, RXN0-746, AIRS-RXN Unpredicted: 0 Entire module: 19 (73%)GDPKIN-RXN, GUANYL-KIN-RXN, ADENYL-KIN-RXN, IMPCYCLOHYDROLASE-RXN, RXN0-743, GMP-SYN-GLUT-RXN, GARTRANSFORMYL2-RXN, ADENYLOSUCCINATE-SYNTHASE-RXN, AICARSYN-RXN, DADPKIN-RXN, ADPREDUCT-RXN, IMP-DEHYDROG-RXN, DGDPKIN-RXN, GDPREDUCT-RXN, AMPSYN-RXN, FGAMSYN-RXN, SAICARSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN Blocked rxns: 0 Essential rxns: 11PRPPAMIDOTRANS-RXN, GUANYL-KIN-RXN, AMPSYN-RXN, FGAMSYN-RXN, ADENYLOSUCCINATE-SYNTHASE-RXN, SAICARSYN-RXN, RXN0-743, GLYRIBONUCSYN-RXN, AICARSYN-RXN, IMPCYCLOHYDROLASE-RXN, GMP-SYN-GLUT-RXN	Annotated: 0 (0%) Annot. missing: 2 (100%)CATAL-RXN, SUPEROX-DISMUT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (100%)DTDPDEHYDRHAMEPIM-RXN, DTDPGLUCOSEPP-RXN, DTDPGLUCDEHYDRAT-RXN, DTDPDEHYRHAMREDUCT-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)DTDPDEHYDRHAMEPIM-RXN, DTDPGLUCOSEPP-RXN, DTDPDEHYRHAMREDUCT-RXN, DTDPGLUCDEHYDRAT-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 8 (80%)DTDPGLUCOSEPP-RXN, TDPFUCACTRANS-RXN, UDPGLCNACEPIM-RXN, RFFTRANS-RXN, UDPMANACATRANS-RXN, UDPMANNACADEHYDROG-RXN, FUC4NACTRANS-RXN, DTDPGLUCDEHYDRAT-RXN Annot. missing: 2 (20%)GLCNACPTRANS-RXN, TRANS-RXN0-279 Unpredicted: 0 Entire module: 8 (80%)DTDPGLUCOSEPP-RXN, TDPFUCACTRANS-RXN, UDPGLCNACEPIM-RXN, RFFTRANS-RXN, UDPMANACATRANS-RXN, UDPMANNACADEHYDROG-RXN, FUC4NACTRANS-RXN, DTDPGLUCDEHYDRAT-RXN Blocked rxns: 0 Essential rxns: 0

	enterobactin biosynthesis	Entner-Doudoroff pathway I	ethanol degradation I	fatty acid β-oxidation I	fatty acid elongation -- saturated	superpathway of fatty acid biosynthesis initiation (E. coli)	mixed acid fermentation	superpathway of fucose and rhamnose degradation	fucose degradation	superpathway of hexuronide and hexuronate degradation
e_coli_core	Annotated: 0 (0%) Annot. missing: 11 (100%)ISOCHORMAT-RXN, DHBDEHYD-RXN, ISOCHORSYN-RXN, ENTG-RXN, RXN-15891, ENTF-RXN, RXN-15890, RXN-15889, RXN0-6442, ENTDB-RXN, DHBAMPLIG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)KDPGALDOL-RXN, PGLUCONDEHYDRAT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)ACYLCOADEHYDROG-RXN, ENOYL-COA-DELTA-ISOM-RXN, KETOACYLCOATHIOL-RXN, ENOYL-COA-HYDRAT-RXN, ACYLCOASYN-RXN, OHACYL-COA-DEHYDROG-RXN, OHBUTYRYL-COA-EPIM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)3-OXOACYL-ACP-REDUCT-RXN, 3-OXOACYL-ACP-SYNTH-RXN, 3-HYDROXYDECANOYL-ACP-DEHYDR-RXN, ENOYL-ACP-REDUCT-NADH-RXN, ENOYL-ACP-REDUCT-NADPH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)ACP-S-ACETYLTRANSFER-RXN, 2.3.1.180-RXN, MALONYL-ACPDECARBOX-RXN, MALONYL-COA-ACP-TRANSACYL-RXN, RXN0-5055, 3-OXOACYL-ACP-SYNTH-BASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 11 (69%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN, DLACTDEHYDROGNAD-RXN, PYRUVFORMLY-RXN, CITSYN-RXN, PHOSACETYLTRANS-RXN, ACETATEKIN-RXN, ACONITATEDEHYDR-RXN, MALATE-DEH-RXN, FUMHYDR-RXN, PEPDEPHOS-RXN Annot. missing: 5 (31%)R601-RXN, FHLMULTI-RXN, ACONITATEHYDR-RXN, ISOCITDEH-RXN, PEPCARBOX-RXN Unpredicted: 0 Entire module: 11 (69%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN, DLACTDEHYDROGNAD-RXN, PYRUVFORMLY-RXN, CITSYN-RXN, PHOSACETYLTRANS-RXN, ACETATEKIN-RXN, ACONITATEDEHYDR-RXN, MALATE-DEH-RXN, FUMHYDR-RXN, PEPDEPHOS-RXN Blocked rxns: 0 Essential rxns: 2CITSYN-RXN, ACONITATEDEHYDR-RXN	Annotated: 0 (0%) Annot. missing: 12 (100%)FUCISOM-RXN, RHAMNULPALDOL-RXN, L-LACTDEHYDROGFMN-RXN, RXN-15356, FUCPALDOL-RXN, RHAMNISOM-RXN, LACTALDREDUCT-RXN, RHAMNULOKIN-RXN, RXN0-5298, LACTALDDEHYDROG-RXN, RXN0-5306, FUCULOKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)FUCULOKIN-RXN, FUCISOM-RXN, RXN0-5298, FUCPALDOL-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 10 (100%)DEOXYGLUCONOKIN-RXN, ALTRO-OXIDOREDUCT-RXN, ALTRODEHYDRAT-RXN, MANNONOXIDOREDUCT-RXN, GALACTUROISOM-RXN, BETA-GLUCURONID-RXN, KDPGALDOL-RXN, MANNONDEHYDRAT-RXN, GLUCUROISOM-RXN, RXN-14693 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 5 (46%)ISOCHORMAT-RXN, DHBDEHYD-RXN, ISOCHORSYN-RXN, ENTF-RXN, DHBAMPLIG-RXN Annot. missing: 6 (54%)ENTG-RXN, RXN-15891, RXN-15890, RXN-15889, RXN0-6442, ENTDB-RXN Unpredicted: 0 Entire module: 5 (46%)ISOCHORMAT-RXN, DHBDEHYD-RXN, ISOCHORSYN-RXN, ENTF-RXN, DHBAMPLIG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)KDPGALDOL-RXN, PGLUCONDEHYDRAT-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)KDPGALDOL-RXN, PGLUCONDEHYDRAT-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)ACYLCOADEHYDROG-RXN, ENOYL-COA-DELTA-ISOM-RXN, KETOACYLCOATHIOL-RXN, ENOYL-COA-HYDRAT-RXN, ACYLCOASYN-RXN, OHACYL-COA-DEHYDROG-RXN, OHBUTYRYL-COA-EPIM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)3-OXOACYL-ACP-REDUCT-RXN, 3-OXOACYL-ACP-SYNTH-RXN, 3-HYDROXYDECANOYL-ACP-DEHYDR-RXN, ENOYL-ACP-REDUCT-NADH-RXN, ENOYL-ACP-REDUCT-NADPH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (83%)ACP-S-ACETYLTRANSFER-RXN, 2.3.1.180-RXN, MALONYL-ACPDECARBOX-RXN, MALONYL-COA-ACP-TRANSACYL-RXN, 3-OXOACYL-ACP-SYNTH-BASE-RXN Annot. missing: 1 (17%)RXN0-5055 Unpredicted: 0 Entire module: 5 (83%)ACP-S-ACETYLTRANSFER-RXN, 2.3.1.180-RXN, MALONYL-ACPDECARBOX-RXN, MALONYL-COA-ACP-TRANSACYL-RXN, 3-OXOACYL-ACP-SYNTH-BASE-RXN Blocked rxns: 0 Essential rxns: 1MALONYL-COA-ACP-TRANSACYL-RXN	Annotated: 11 (69%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN, DLACTDEHYDROGNAD-RXN, PYRUVFORMLY-RXN, CITSYN-RXN, PHOSACETYLTRANS-RXN, ACETATEKIN-RXN, ACONITATEDEHYDR-RXN, MALATE-DEH-RXN, FUMHYDR-RXN, PEPDEPHOS-RXN Annot. missing: 5 (31%)R601-RXN, FHLMULTI-RXN, ACONITATEHYDR-RXN, ISOCITDEH-RXN, PEPCARBOX-RXN Unpredicted: 0 Entire module: 11 (69%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN, DLACTDEHYDROGNAD-RXN, PYRUVFORMLY-RXN, CITSYN-RXN, PHOSACETYLTRANS-RXN, ACETATEKIN-RXN, ACONITATEDEHYDR-RXN, MALATE-DEH-RXN, FUMHYDR-RXN, PEPDEPHOS-RXN Blocked rxns: 0 Essential rxns: 2CITSYN-RXN, ACONITATEDEHYDR-RXN	Annotated: 5 (42%)FUCPALDOL-RXN, RHAMNULPALDOL-RXN, LACTALDREDUCT-RXN, LACTALDDEHYDROG-RXN, FUCULOKIN-RXN Annot. missing: 7 (58%)FUCISOM-RXN, RXN-15356, L-LACTDEHYDROGFMN-RXN, RHAMNULOKIN-RXN, RXN0-5298, RHAMNISOM-RXN, RXN0-5306 Unpredicted: 0 Entire module: 5 (42%)FUCPALDOL-RXN, RHAMNULPALDOL-RXN, LACTALDREDUCT-RXN, LACTALDDEHYDROG-RXN, FUCULOKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (50%)FUCULOKIN-RXN, FUCPALDOL-RXN Annot. missing: 2 (50%)FUCISOM-RXN, RXN0-5298 Unpredicted: 0 Entire module: 2 (50%)FUCULOKIN-RXN, FUCPALDOL-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (60%)DEOXYGLUCONOKIN-RXN, ALTRO-OXIDOREDUCT-RXN, ALTRODEHYDRAT-RXN, MANNONOXIDOREDUCT-RXN, KDPGALDOL-RXN, MANNONDEHYDRAT-RXN Annot. missing: 4 (40%)GLUCUROISOM-RXN, GALACTUROISOM-RXN, BETA-GLUCURONID-RXN, RXN-14693 Unpredicted: 0 Entire module: 6 (60%)DEOXYGLUCONOKIN-RXN, ALTRO-OXIDOREDUCT-RXN, ALTRODEHYDRAT-RXN, MANNONOXIDOREDUCT-RXN, KDPGALDOL-RXN, MANNONDEHYDRAT-RXN Blocked rxns: 0 Essential rxns: 0

	D-galactarate degradation I	D-galactonate degradation	galactitol degradation	D-galacturonate degradation I	superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation	L-glutamine biosynthesis I	N-acetylglucosamine degradation I	D-glucarate degradation I	superpathway of D-glucarate and D-galactarate degradation	gluconeogenesis I
e_coli_core	Annotated: 0 (0%) Annot. missing: 4 (100%)GALACTARDEHYDRA-RXN, GKI-RXN, TSA-REDUCT-RXN, KDGALDOL-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)DEHYDDEOXGALACTKIN-RXN, DEHYDDEOXPHOSGALACT-ALDOL-RXN, GALACTONDEHYDRAT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)TRANS-RXN-161, TAGAKIN-RXN, TAGAALDOL-RXN, 1.1.1.251-RXN, RXN-16992 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)DEOXYGLUCONOKIN-RXN, ALTRO-OXIDOREDUCT-RXN, ALTRODEHYDRAT-RXN, GALACTUROISOM-RXN, KDPGALDOL-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)NANK-RXN, NAG6PDEACET-RXN, GLUCOSAMINE-6-P-DEAMIN-RXN, NANE-RXN, ACNEULY-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)GLUTAMINESYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GLUTAMINESYN-RXN Blocked rxns: 0 Essential rxns: 1GLUTAMINESYN-RXN	Annotated: 0 (0%) Annot. missing: 2 (100%)GLUCOSAMINE-6-P-DEAMIN-RXN, NAG6PDEACET-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)GLUCARDEHYDRA-RXN, GKI-RXN, TSA-REDUCT-RXN, KDGALDOL-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)GALACTARDEHYDRA-RXN, GKI-RXN, GLUCARDEHYDRA-RXN, KDGALDOL-RXN, TSA-REDUCT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 13 (100%)GAPOXNPHOSPHN-RXN, F16BDEPHOS-RXN, PGLUCISOM-RXN, 1.1.1.39-RXN, PHOSGLYPHOS-RXN, PEPCARBOXYKIN-RXN, TRIOSEPISOMERIZATION-RXN, MALIC-NADP-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, MALATE-DEH-RXN, PEPSYNTH-RXN, 3PGAREARR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 13 (100%)GAPOXNPHOSPHN-RXN, F16BDEPHOS-RXN, PGLUCISOM-RXN, 1.1.1.39-RXN, PHOSGLYPHOS-RXN, PEPCARBOXYKIN-RXN, TRIOSEPISOMERIZATION-RXN, MALIC-NADP-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, MALATE-DEH-RXN, PEPSYNTH-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 42PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PHOSGLYPHOS-RXN, 3PGAREARR-RXN
iAF1260	Annotated: 3 (75%)GALACTARDEHYDRA-RXN, GKI-RXN, KDGALDOL-RXN Annot. missing: 1 (25%)TSA-REDUCT-RXN Unpredicted: 0 Entire module: 3 (75%)GALACTARDEHYDRA-RXN, GKI-RXN, KDGALDOL-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)DEHYDDEOXGALACTKIN-RXN, DEHYDDEOXPHOSGALACT-ALDOL-RXN, GALACTONDEHYDRAT-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)DEHYDDEOXGALACTKIN-RXN, DEHYDDEOXPHOSGALACT-ALDOL-RXN, GALACTONDEHYDRAT-RXN Blocked rxns: 3DEHYDDEOXGALACTKIN-RXN, DEHYDDEOXPHOSGALACT-ALDOL-RXN, GALACTONDEHYDRAT-RXN Essential rxns: 0	Annotated: 2 (40%)TAGAALDOL-RXN, TAGAKIN-RXN Annot. missing: 3 (60%)1.1.1.251-RXN, TRANS-RXN-161, RXN-16992 Unpredicted: 0 Entire module: 2 (40%)TAGAALDOL-RXN, TAGAKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (80%)ALTRODEHYDRAT-RXN, DEOXYGLUCONOKIN-RXN, ALTRO-OXIDOREDUCT-RXN, KDPGALDOL-RXN Annot. missing: 1 (20%)GALACTUROISOM-RXN Unpredicted: 0 Entire module: 4 (80%)ALTRODEHYDRAT-RXN, DEOXYGLUCONOKIN-RXN, ALTRO-OXIDOREDUCT-RXN, KDPGALDOL-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (80%)NANE-RXN, NAG6PDEACET-RXN, ACNEULY-RXN, NANK-RXN Annot. missing: 1 (20%)GLUCOSAMINE-6-P-DEAMIN-RXN Unpredicted: 0 Entire module: 4 (80%)NANE-RXN, NAG6PDEACET-RXN, ACNEULY-RXN, NANK-RXN Blocked rxns: 1ACNEULY-RXN Essential rxns: 0	Annotated: 1 (100%)GLUTAMINESYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GLUTAMINESYN-RXN Blocked rxns: 0 Essential rxns: 1GLUTAMINESYN-RXN	Annotated: 1 (50%)NAG6PDEACET-RXN Annot. missing: 1 (50%)GLUCOSAMINE-6-P-DEAMIN-RXN Unpredicted: 0 Entire module: 1 (50%)NAG6PDEACET-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (75%)GKI-RXN, GLUCARDEHYDRA-RXN, KDGALDOL-RXN Annot. missing: 1 (25%)TSA-REDUCT-RXN Unpredicted: 0 Entire module: 3 (75%)GKI-RXN, GLUCARDEHYDRA-RXN, KDGALDOL-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (80%)GALACTARDEHYDRA-RXN, GKI-RXN, GLUCARDEHYDRA-RXN, KDGALDOL-RXN Annot. missing: 1 (20%)TSA-REDUCT-RXN Unpredicted: 0 Entire module: 4 (80%)GALACTARDEHYDRA-RXN, GKI-RXN, GLUCARDEHYDRA-RXN, KDGALDOL-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 13 (100%)GAPOXNPHOSPHN-RXN, F16BDEPHOS-RXN, PGLUCISOM-RXN, 1.1.1.39-RXN, PHOSGLYPHOS-RXN, PEPCARBOXYKIN-RXN, TRIOSEPISOMERIZATION-RXN, MALIC-NADP-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, MALATE-DEH-RXN, PEPSYNTH-RXN, 3PGAREARR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 13 (100%)GAPOXNPHOSPHN-RXN, F16BDEPHOS-RXN, PGLUCISOM-RXN, 1.1.1.39-RXN, PHOSGLYPHOS-RXN, PEPCARBOXYKIN-RXN, TRIOSEPISOMERIZATION-RXN, MALIC-NADP-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, MALATE-DEH-RXN, PEPSYNTH-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 0

	D-gluconate degradation	glucose and glucose-1-phosphate degradation	superpathway of β-D-glucuronosides degradation	L-glutamate degradation VII (to butanoate)	glutathione-glutaredoxin redox reactions	L-glutamine degradation I	glutathione biosynthesis	L-glutamate degradation II	L-ornithine biosynthesis I	L-glutamate biosynthesis I
e_coli_core	Annotated: 0 (0%) Annot. missing: 1 (100%)GLUCONOKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)PHOSPHOGLUCMUT-RXN, GLUCOKIN-RXN, RXN0-6373, GLUCONOLACT-RXN, GLUCOSE-1-PHOSPHAT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)DEOXYGLUCONOKIN-RXN, MANNONOXIDOREDUCT-RXN, BETA-GLUCURONID-RXN, KDPGALDOL-RXN, MANNONDEHYDRAT-RXN, GLUCUROISOM-RXN, RXN-14693 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PRODISULFREDUCT-A-RXN, RXN0-7271 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)GLUTAMIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GLUTAMIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)GLUTATHIONE-SYN-RXN, GLUTCYSLIG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)ASPARTASE-RXN, ASPAMINOTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)N-ACETYLTRANSFER-RXN, ACETYLORNTRANSAM-RXN, N-ACETYLGLUTPREDUCT-RXN, ACETYLORNDEACET-RXN, ACETYLGLUTKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)GLUTAMATESYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GLUTAMATESYN-RXN Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 1 (100%)GLUCONOKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GLUCONOKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (60%)PHOSPHOGLUCMUT-RXN, GLUCOKIN-RXN, GLUCOSE-1-PHOSPHAT-RXN Annot. missing: 2 (40%)RXN0-6373, GLUCONOLACT-RXN Unpredicted: 0 Entire module: 3 (60%)PHOSPHOGLUCMUT-RXN, GLUCOKIN-RXN, GLUCOSE-1-PHOSPHAT-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (57%)MANNONDEHYDRAT-RXN, MANNONOXIDOREDUCT-RXN, DEOXYGLUCONOKIN-RXN, KDPGALDOL-RXN Annot. missing: 3 (43%)GLUCUROISOM-RXN, BETA-GLUCURONID-RXN, RXN-14693 Unpredicted: 0 Entire module: 4 (57%)MANNONDEHYDRAT-RXN, MANNONOXIDOREDUCT-RXN, DEOXYGLUCONOKIN-RXN, KDPGALDOL-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2RXN-11662, RXN-11667 Entire module: 3 (25%)ACETYL-COA-ACETYLTRANSFER-RXN, RXN-11662, RXN-11667 Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PRODISULFREDUCT-A-RXN, RXN0-7271 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)GLUTAMIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GLUTAMIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)GLUTATHIONE-SYN-RXN, GLUTCYSLIG-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)GLUTATHIONE-SYN-RXN, GLUTCYSLIG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)ASPARTASE-RXN, ASPAMINOTRANS-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)ASPARTASE-RXN, ASPAMINOTRANS-RXN Blocked rxns: 0 Essential rxns: 1ASPAMINOTRANS-RXN	Annotated: 5 (100%)N-ACETYLTRANSFER-RXN, ACETYLORNTRANSAM-RXN, N-ACETYLGLUTPREDUCT-RXN, ACETYLORNDEACET-RXN, ACETYLGLUTKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)N-ACETYLTRANSFER-RXN, ACETYLORNTRANSAM-RXN, N-ACETYLGLUTPREDUCT-RXN, ACETYLORNDEACET-RXN, ACETYLGLUTKIN-RXN Blocked rxns: 0 Essential rxns: 5N-ACETYLTRANSFER-RXN, ACETYLORNTRANSAM-RXN, N-ACETYLGLUTPREDUCT-RXN, ACETYLORNDEACET-RXN, ACETYLGLUTKIN-RXN	Annotated: 1 (100%)GLUTAMATESYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GLUTAMATESYN-RXN Blocked rxns: 0 Essential rxns: 0

	L-glutamate biosynthesis III	glycerol degradation V	glycine cleavage	glycogen biosynthesis I (from ADP-D-Glucose)	superpathway of glycol metabolism and degradation	glycolate and glyoxylate degradation I	glycolysis I (from glucose 6-phosphate)	superpathway of glycolysis and the Entner-Doudoroff pathway	superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	glycolate and glyoxylate degradation II
e_coli_core	Annotated: 1 (100%)GLUTDEHYD-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GLUTDEHYD-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)2.7.1.121-RXN, GLYCDEH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-8629, GCVP-RXN, GCVT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)PHOSPHOGLUCMUT-RXN, GLYCOGENSYN-RXN, GLYCOGEN-BRANCH-RXN, GLUC1PADENYLTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (14%)MALSYN-RXN Annot. missing: 6 (86%)GKI-RXN, GLYCOLALD-DEHYDROG-RXN, GLYCOLATEDEHYDRO-RXN, GLYCOLALDREDUCT-RXN, GLYOCARBOLIG-RXN, RXN0-5289 Unpredicted: 0 Entire module: 1 (14%)MALSYN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN0-5289, GKI-RXN, GLYOCARBOLIG-RXN, GLYCOLATEDEHYDRO-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 12 (100%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PGLUCISOM-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 12 (100%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PGLUCISOM-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 5RXN-15513, PHOSGLYPHOS-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, 3PGAREARR-RXN	Annotated: 14 (88%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PGLUCISOM-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, 6PGLUCONOLACT-RXN, GLU6PDEHYDROG-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Annot. missing: 2 (12%)PGLUCONDEHYDRAT-RXN, KDPGALDOL-RXN Unpredicted: 0 Entire module: 14 (88%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PGLUCISOM-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, 6PGLUCONOLACT-RXN, GLU6PDEHYDROG-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 5RXN-15513, PHOSGLYPHOS-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, 3PGAREARR-RXN	Annotated: 20 (80%)RXN-15513, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, CITSYN-RXN, PYRUVDEH-RXN, ACONITATEDEHYDR-RXN, 2PGADEHYDRAT-RXN, PEPSYNTH-RXN, 3PGAREARR-RXN, MALSYN-RXN, GAPOXNPHOSPHN-RXN, PGLUCISOM-RXN, SUCCCOASYN-RXN, F16BDEPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, FUMHYDR-RXN, 2OXOGLUTARATEDEH-RXN, MALATE-DEH-RXN, PEPDEPHOS-RXN Annot. missing: 5 (20%)ISOCIT-CLEAV-RXN, MALATE-DEHYDROGENASE-ACCEPTOR-RXN, SUCCINATE-DEHYDROGENASE-UBIQUINONE-RXN, ACONITATEHYDR-RXN, ISOCITDEH-RXN Unpredicted: 0 Entire module: 20 (80%)RXN-15513, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, CITSYN-RXN, PYRUVDEH-RXN, ACONITATEDEHYDR-RXN, 2PGADEHYDRAT-RXN, PEPSYNTH-RXN, 3PGAREARR-RXN, MALSYN-RXN, GAPOXNPHOSPHN-RXN, PGLUCISOM-RXN, SUCCCOASYN-RXN, F16BDEPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, FUMHYDR-RXN, 2OXOGLUTARATEDEH-RXN, MALATE-DEH-RXN, PEPDEPHOS-RXN Blocked rxns: 0 Essential rxns: 7RXN-15513, PHOSGLYPHOS-RXN, CITSYN-RXN, ACONITATEDEHYDR-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, 3PGAREARR-RXN	Annotated: 1 (50%)MALSYN-RXN Annot. missing: 1 (50%)GLYCOLATEDEHYDRO-RXN Unpredicted: 0 Entire module: 1 (50%)MALSYN-RXN Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 1 (100%)GLUTDEHYD-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GLUTDEHYD-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)2.7.1.121-RXN, GLYCDEH-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)2.7.1.121-RXN, GLYCDEH-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-8629, GCVP-RXN, GCVT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (50%)PHOSPHOGLUCMUT-RXN, GLUC1PADENYLTRANS-RXN Annot. missing: 2 (50%)GLYCOGENSYN-RXN, GLYCOGEN-BRANCH-RXN Unpredicted: 0 Entire module: 2 (50%)PHOSPHOGLUCMUT-RXN, GLUC1PADENYLTRANS-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (71%)GKI-RXN, GLYCOLALD-DEHYDROG-RXN, GLYOCARBOLIG-RXN, MALSYN-RXN, RXN0-5289 Annot. missing: 2 (29%)GLYCOLALDREDUCT-RXN, GLYCOLATEDEHYDRO-RXN Unpredicted: 0 Entire module: 5 (71%)GKI-RXN, GLYCOLALD-DEHYDROG-RXN, GLYOCARBOLIG-RXN, MALSYN-RXN, RXN0-5289 Blocked rxns: 0 Essential rxns: 1GLYCOLALD-DEHYDROG-RXN	Annotated: 3 (75%)RXN0-5289, GKI-RXN, GLYOCARBOLIG-RXN Annot. missing: 1 (25%)GLYCOLATEDEHYDRO-RXN Unpredicted: 0 Entire module: 3 (75%)GLYOCARBOLIG-RXN, GKI-RXN, RXN0-5289 Blocked rxns: 0 Essential rxns: 0	Annotated: 12 (100%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PGLUCISOM-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 12 (100%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PGLUCISOM-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 16 (100%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PGLUCISOM-RXN, PHOSGLYPHOS-RXN, PGLUCONDEHYDRAT-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, 6PGLUCONOLACT-RXN, KDPGALDOL-RXN, GLU6PDEHYDROG-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 16 (100%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PGLUCISOM-RXN, PHOSGLYPHOS-RXN, PGLUCONDEHYDRAT-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, 6PGLUCONOLACT-RXN, KDPGALDOL-RXN, GLU6PDEHYDROG-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 20 (80%)RXN-15513, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, CITSYN-RXN, PYRUVDEH-RXN, ACONITATEDEHYDR-RXN, 2PGADEHYDRAT-RXN, PEPSYNTH-RXN, 3PGAREARR-RXN, MALSYN-RXN, GAPOXNPHOSPHN-RXN, PGLUCISOM-RXN, SUCCCOASYN-RXN, F16BDEPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, FUMHYDR-RXN, 2OXOGLUTARATEDEH-RXN, MALATE-DEH-RXN, PEPDEPHOS-RXN Annot. missing: 5 (20%)ISOCIT-CLEAV-RXN, MALATE-DEHYDROGENASE-ACCEPTOR-RXN, SUCCINATE-DEHYDROGENASE-UBIQUINONE-RXN, ACONITATEHYDR-RXN, ISOCITDEH-RXN Unpredicted: 0 Entire module: 20 (80%)RXN-15513, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, CITSYN-RXN, PYRUVDEH-RXN, ACONITATEDEHYDR-RXN, 2PGADEHYDRAT-RXN, PEPSYNTH-RXN, 3PGAREARR-RXN, MALSYN-RXN, GAPOXNPHOSPHN-RXN, PGLUCISOM-RXN, SUCCCOASYN-RXN, F16BDEPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, FUMHYDR-RXN, 2OXOGLUTARATEDEH-RXN, MALATE-DEH-RXN, PEPDEPHOS-RXN Blocked rxns: 0 Essential rxns: 2CITSYN-RXN, ACONITATEDEHYDR-RXN	Annotated: 1 (50%)MALSYN-RXN Annot. missing: 1 (50%)GLYCOLATEDEHYDRO-RXN Unpredicted: 0 Entire module: 1 (50%)MALSYN-RXN Blocked rxns: 0 Essential rxns: 0

	glyoxylate cycle	glycine biosynthesis I	3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate	heme b biosynthesis I (aerobic)	heme b biosynthesis II (anaerobic)	superpathway of hexitol degradation (bacteria)	L-histidine biosynthesis	L-methionine biosynthesis I	L-threonine biosynthesis	L-homoserine biosynthesis
e_coli_core	Annotated: 4 (67%)MALSYN-RXN, CITSYN-RXN, MALATE-DEH-RXN, ACONITATEDEHYDR-RXN Annot. missing: 2 (33%)ISOCIT-CLEAV-RXN, ACONITATEHYDR-RXN Unpredicted: 0 Entire module: 4 (67%)MALSYN-RXN, CITSYN-RXN, MALATE-DEH-RXN, ACONITATEDEHYDR-RXN Blocked rxns: 0 Essential rxns: 2CITSYN-RXN, ACONITATEDEHYDR-RXN	Annotated: 0 (0%) Annot. missing: 1 (100%)GLYOHMETRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)PHENPRODIOLDEHYDROG-RXN, HCAMULTI-RXN, MHPHYDROXY-RXN, MHPCHYDROL-RXN, 1.13.11.16-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN0-1461, UROGENDECARBOX-RXN, PROTOPORGENOXI-RXN, PROTOHEMEFERROCHELAT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)UROGENDECARBOX-RXN, RXN0-6259, HEMN-RXN, PROTOHEMEFERROCHELAT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 11 (61%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Annot. missing: 7 (39%)SORB6PDEHYDROG-RXN, TRANS-RXN-161, MANNPDEHYDROG-RXN, TAGAKIN-RXN, TAGAALDOL-RXN, 1.1.1.251-RXN, RXN-16992 Unpredicted: 0 Entire module: 11 (61%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 5RXN-15513, PHOSGLYPHOS-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, 3PGAREARR-RXN	Annotated: 0 (0%) Annot. missing: 10 (100%)HISTOLDEHYD-RXN, PRIBFAICARPISOM-RXN, HISTIDPHOS-RXN, GLUTAMIDOTRANS-RXN, ATPPHOSPHORIBOSYLTRANS-RXN, HISTAMINOTRANS-RXN, HISTALDEHYD-RXN, HISTCYCLOHYD-RXN, HISTPRATPHYD-RXN, IMIDPHOSDEHYD-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)RXN-15124, O-SUCCHOMOSERLYASE-RXN, HOMSUCTRAN-RXN, HOMOCYSMETB12-RXN, RXN-15131, HOMOCYSMET-RXN, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)HOMOSERKIN-RXN, THRESYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, HOMOSERDEHYDROG-RXN, ASPARTATEKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 4 (67%)MALSYN-RXN, CITSYN-RXN, MALATE-DEH-RXN, ACONITATEDEHYDR-RXN Annot. missing: 2 (33%)ISOCIT-CLEAV-RXN, ACONITATEHYDR-RXN Unpredicted: 0 Entire module: 4 (67%)MALSYN-RXN, CITSYN-RXN, MALATE-DEH-RXN, ACONITATEDEHYDR-RXN Blocked rxns: 0 Essential rxns: 2CITSYN-RXN, ACONITATEDEHYDR-RXN	Annotated: 0 (0%) Annot. missing: 1 (100%)GLYOHMETRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (80%)HCAMULTI-RXN, PHENPRODIOLDEHYDROG-RXN, MHPHYDROXY-RXN, 1.13.11.16-RXN Annot. missing: 1 (20%)MHPCHYDROL-RXN Unpredicted: 0 Entire module: 4 (80%)HCAMULTI-RXN, PHENPRODIOLDEHYDROG-RXN, MHPHYDROXY-RXN, 1.13.11.16-RXN Blocked rxns: 4HCAMULTI-RXN, PHENPRODIOLDEHYDROG-RXN, MHPHYDROXY-RXN, 1.13.11.16-RXN Essential rxns: 0	Annotated: 3 (75%)RXN0-1461, UROGENDECARBOX-RXN, PROTOHEMEFERROCHELAT-RXN Annot. missing: 1 (25%)PROTOPORGENOXI-RXN Unpredicted: 0 Entire module: 3 (75%)RXN0-1461, UROGENDECARBOX-RXN, PROTOHEMEFERROCHELAT-RXN Blocked rxns: 0 Essential rxns: 2UROGENDECARBOX-RXN, PROTOHEMEFERROCHELAT-RXN	Annotated: 3 (75%)UROGENDECARBOX-RXN, HEMN-RXN, PROTOHEMEFERROCHELAT-RXN Annot. missing: 1 (25%)RXN0-6259 Unpredicted: 0 Entire module: 3 (75%)UROGENDECARBOX-RXN, HEMN-RXN, PROTOHEMEFERROCHELAT-RXN Blocked rxns: 0 Essential rxns: 2UROGENDECARBOX-RXN, PROTOHEMEFERROCHELAT-RXN	Annotated: 14 (78%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, MANNPDEHYDROG-RXN, TRIOSEPISOMERIZATION-RXN, TAGAKIN-RXN, F16ALDOLASE-RXN, TAGAALDOL-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Annot. missing: 4 (22%)SORB6PDEHYDROG-RXN, 1.1.1.251-RXN, TRANS-RXN-161, RXN-16992 Unpredicted: 0 Entire module: 14 (78%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, MANNPDEHYDROG-RXN, TRIOSEPISOMERIZATION-RXN, TAGAKIN-RXN, F16ALDOLASE-RXN, TAGAALDOL-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 8 (80%)PRIBFAICARPISOM-RXN, HISTIDPHOS-RXN, GLUTAMIDOTRANS-RXN, ATPPHOSPHORIBOSYLTRANS-RXN, HISTAMINOTRANS-RXN, HISTCYCLOHYD-RXN, HISTPRATPHYD-RXN, IMIDPHOSDEHYD-RXN Annot. missing: 2 (20%)HISTALDEHYD-RXN, HISTOLDEHYD-RXN Unpredicted: 0 Entire module: 8 (80%)PRIBFAICARPISOM-RXN, HISTIDPHOS-RXN, GLUTAMIDOTRANS-RXN, ATPPHOSPHORIBOSYLTRANS-RXN, HISTAMINOTRANS-RXN, HISTCYCLOHYD-RXN, HISTPRATPHYD-RXN, IMIDPHOSDEHYD-RXN Blocked rxns: 0 Essential rxns: 8PRIBFAICARPISOM-RXN, HISTIDPHOS-RXN, GLUTAMIDOTRANS-RXN, ATPPHOSPHORIBOSYLTRANS-RXN, HISTAMINOTRANS-RXN, HISTCYCLOHYD-RXN, HISTPRATPHYD-RXN, IMIDPHOSDEHYD-RXN	Annotated: 2 (29%)O-SUCCHOMOSERLYASE-RXN, HOMSUCTRAN-RXN Annot. missing: 5 (71%)RXN-15124, HOMOCYSMETB12-RXN, RXN-15131, HOMOCYSMET-RXN, RXN-15127 Unpredicted: 0 Entire module: 2 (29%)O-SUCCHOMOSERLYASE-RXN, HOMSUCTRAN-RXN Blocked rxns: 0 Essential rxns: 2O-SUCCHOMOSERLYASE-RXN, HOMSUCTRAN-RXN	Annotated: 2 (100%)HOMOSERKIN-RXN, THRESYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)HOMOSERKIN-RXN, THRESYN-RXN Blocked rxns: 0 Essential rxns: 2HOMOSERKIN-RXN, THRESYN-RXN	Annotated: 2 (67%)ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, ASPARTATEKIN-RXN Annot. missing: 1 (33%)HOMOSERDEHYDROG-RXN Unpredicted: 0 Entire module: 2 (67%)ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, ASPARTATEKIN-RXN Blocked rxns: 0 Essential rxns: 2ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, ASPARTATEKIN-RXN

	L-idonate degradation	L-isoleucine biosynthesis I (from threonine)	(Kdo)₂-lipid A biosynthesis I	superpathway of (Kdo)₂-lipid A biosynthesis	Kdo transfer to lipid IV_A I	ketogluconate metabolism	L-cysteine degradation II	L-leucine biosynthesis	Lipid A-core biosynthesis	superpathway of lipopolysaccharide biosynthesis
e_coli_core	Annotated: 0 (0%) Annot. missing: 3 (100%)GLUCONOKIN-RXN, 1.1.1.264-RXN, GLUCONATE-5-DEHYDROGENASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)RXN-15121, RXN-15122, ACETOOHBUTREDUCTOISOM-RXN, RXN-15123, DIHYDROXYMETVALDEHYDRAT-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN, ACETOOHBUTSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)MYRISTOYLACYLTRAN-RXN, LAUROYLACYLTRAN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 17 (100%)MYRISTOYLACYLTRAN-RXN, KDO-8PSYNTH-RXN, LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, MYRPALMTRAN-RXN, RXN-16804, DARAB5PISOM-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, KDO-8PPHOSPHAT-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN, KDOTRANS-RXN, LIPIDXSYNTHESIS-RXN, PALMITOTRANS-RXN, LAUROYLACYLTRAN-RXN, CPM-KDOSYNTH-RXN, KDOTRANS2-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)KDOTRANS-RXN, KDOTRANS2-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)YIAE1-RXN, RXN0-7020, 1.1.1.264-RXN, GLUCONATE-5-DEHYDROGENASE-RXN, GLUCONOKIN-RXN, RXN0-7021, 1.1.1.215-RXN, 1.1.1.274-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-15124, RXN-15129, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)BRANCHED-CHAINAMINOTRANSFERLEU-RXN, RXN-8991, 3-ISOPROPYLMALISOM-RXN, RXN-7800, 2-ISOPROPYLMALATESYN-RXN, 3-ISOPROPYLMALDEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 11 (100%)RXN0-5118, RXN0-5122, RXN-19737, RXN0-5127, RXN0-5123, RXN0-5121, RXN0-5125, RXN0-5061, RXN0-5120, RXN0-5124, RXN0-5126 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 26 (100%)KDO-8PSYNTH-RXN, RXN0-5122, RXN-19737, KDO-8PPHOSPHAT-RXN, LIPIDXSYNTHESIS-RXN, LAUROYLACYLTRAN-RXN, RXN0-5061, KDOTRANS2-RXN, RXN0-5120, MYRISTOYLACYLTRAN-RXN, LIPIDADISACCHARIDESYNTH-RXN, DARAB5PISOM-RXN, TETRAACYLDISACC4KIN-RXN, RXN0-5125, CPM-KDOSYNTH-RXN, RXN-16804, RXN0-5118, UDPNACETYLGLUCOSAMACYLTRANS-RXN, RXN0-5127, RXN0-5123, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN, RXN0-5124, RXN0-5126, UDPACYLGLCNACDEACETYL-RXN, RXN0-5121, KDOTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 1 (33%)GLUCONOKIN-RXN Annot. missing: 2 (67%)1.1.1.264-RXN, GLUCONATE-5-DEHYDROGENASE-RXN Unpredicted: 0 Entire module: 1 (33%)GLUCONOKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (57%)DIHYDROXYMETVALDEHYDRAT-RXN, ACETOOHBUTSYN-RXN, ACETOOHBUTREDUCTOISOM-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN Annot. missing: 3 (43%)RXN-15121, RXN-15122, RXN-15123 Unpredicted: 0 Entire module: 4 (57%)DIHYDROXYMETVALDEHYDRAT-RXN, ACETOOHBUTSYN-RXN, ACETOOHBUTREDUCTOISOM-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN Blocked rxns: 0 Essential rxns: 4DIHYDROXYMETVALDEHYDRAT-RXN, ACETOOHBUTSYN-RXN, ACETOOHBUTREDUCTOISOM-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN	Annotated: 0 (0%) Annot. missing: 2 (100%)MYRISTOYLACYLTRAN-RXN, LAUROYLACYLTRAN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 11 (65%)LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, DARAB5PISOM-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, KDO-8PPHOSPHAT-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN, KDOTRANS-RXN, LIPIDXSYNTHESIS-RXN, CPM-KDOSYNTH-RXN, KDOTRANS2-RXN Annot. missing: 6 (35%)MYRISTOYLACYLTRAN-RXN, KDO-8PSYNTH-RXN, RXN-16804, MYRPALMTRAN-RXN, PALMITOTRANS-RXN, LAUROYLACYLTRAN-RXN Unpredicted: 0 Entire module: 11 (65%)LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, DARAB5PISOM-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, KDO-8PPHOSPHAT-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN, KDOTRANS-RXN, LIPIDXSYNTHESIS-RXN, CPM-KDOSYNTH-RXN, KDOTRANS2-RXN Blocked rxns: 0 Essential rxns: 11LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, DARAB5PISOM-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, KDO-8PPHOSPHAT-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN, KDOTRANS-RXN, LIPIDXSYNTHESIS-RXN, CPM-KDOSYNTH-RXN, KDOTRANS2-RXN	Annotated: 2 (100%)KDOTRANS-RXN, KDOTRANS2-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)KDOTRANS-RXN, KDOTRANS2-RXN Blocked rxns: 0 Essential rxns: 2KDOTRANS-RXN, KDOTRANS2-RXN	Annotated: 3 (38%)GLUCONOKIN-RXN, 1.1.1.215-RXN, YIAE1-RXN Annot. missing: 5 (62%)RXN0-7020, 1.1.1.264-RXN, GLUCONATE-5-DEHYDROGENASE-RXN, RXN0-7021, 1.1.1.274-RXN Unpredicted: 0 Entire module: 3 (38%)GLUCONOKIN-RXN, 1.1.1.215-RXN, YIAE1-RXN Blocked rxns: 21.1.1.215-RXN, YIAE1-RXN Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-15124, RXN-15129, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (100%)BRANCHED-CHAINAMINOTRANSFERLEU-RXN, RXN-8991, 3-ISOPROPYLMALISOM-RXN, RXN-7800, 2-ISOPROPYLMALATESYN-RXN, 3-ISOPROPYLMALDEHYDROG-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)BRANCHED-CHAINAMINOTRANSFERLEU-RXN, RXN-8991, RXN-7800, 2-ISOPROPYLMALATESYN-RXN, 3-ISOPROPYLMALDEHYDROG-RXN Blocked rxns: 0 Essential rxns: 6BRANCHED-CHAINAMINOTRANSFERLEU-RXN, RXN-8991, 3-ISOPROPYLMALISOM-RXN, RXN-7800, 2-ISOPROPYLMALATESYN-RXN, 3-ISOPROPYLMALDEHYDROG-RXN	Annotated: 2 (18%)RXN0-5061, RXN0-5118 Annot. missing: 9 (82%)RXN0-5122, RXN-19737, RXN0-5127, RXN0-5123, RXN0-5121, RXN0-5125, RXN0-5120, RXN0-5124, RXN0-5126 Unpredicted: 0 Entire module: 2 (18%)RXN0-5061, RXN0-5118 Blocked rxns: 0 Essential rxns: 0	Annotated: 13 (50%)LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, RXN0-5118, DARAB5PISOM-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, KDO-8PPHOSPHAT-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN, LIPIDXSYNTHESIS-RXN, KDOTRANS-RXN, RXN0-5061, CPM-KDOSYNTH-RXN, KDOTRANS2-RXN Annot. missing: 13 (50%)MYRISTOYLACYLTRAN-RXN, KDO-8PSYNTH-RXN, RXN-16804, RXN0-5122, RXN-19737, RXN0-5127, RXN0-5123, RXN0-5121, RXN0-5125, LAUROYLACYLTRAN-RXN, RXN0-5120, RXN0-5124, RXN0-5126 Unpredicted: 0 Entire module: 13 (50%)LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, RXN0-5118, DARAB5PISOM-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, KDO-8PPHOSPHAT-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN, LIPIDXSYNTHESIS-RXN, KDOTRANS-RXN, RXN0-5061, CPM-KDOSYNTH-RXN, KDOTRANS2-RXN Blocked rxns: 0 Essential rxns: 11LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, DARAB5PISOM-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, KDO-8PPHOSPHAT-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN, LIPIDXSYNTHESIS-RXN, KDOTRANS-RXN, CPM-KDOSYNTH-RXN, KDOTRANS2-RXN

	L-lyxose degradation	D-mannose degradation	mannitol degradation I	menaquinol-8 biosynthesis	superpathway of S-adenosyl-L-methionine biosynthesis	superpathway of methylglyoxal degradation	methylgallate degradation	L-homoserine and L-methionine biosynthesis	methylglyoxal degradation VI	NAD phosphorylation and dephosphorylation
e_coli_core	Annotated: 0 (0%) Annot. missing: 4 (100%)RIBULPEPIM-RXN, LYXK-RXN, LXULRU5P-RXN, LYXISOM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)MANNPISOM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)MANNPDEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ADOMET-DMK-METHYLTRANSFER-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 11 (100%)S-ADENMETSYN-RXN, RXN-15124, ASPARTATEKIN-RXN, O-SUCCHOMOSERLYASE-RXN, HOMSUCTRAN-RXN, HOMOCYSMETB12-RXN, HOMOSERDEHYDROG-RXN, RXN-15131, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, HOMOCYSMET-RXN, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)RXN0-4281, L-LACTDEHYDROGFMN-RXN, DLACTDEHYDROGFAD-RXN, RXN-8632, 1.1.1.283-RXN, GLYOXII-RXN, GLYOXI-RXN, LACTALDDEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 10 (100%)RXN-15124, ASPARTATEKIN-RXN, O-SUCCHOMOSERLYASE-RXN, HOMSUCTRAN-RXN, HOMOCYSMETB12-RXN, HOMOSERDEHYDROG-RXN, RXN-15131, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, HOMOCYSMET-RXN, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-5822, NAD-KIN-RXN, TRANS-RXN0-277 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 3 (75%)RIBULPEPIM-RXN, LYXK-RXN, LXULRU5P-RXN Annot. missing: 1 (25%)LYXISOM-RXN Unpredicted: 0 Entire module: 3 (75%)RIBULPEPIM-RXN, LYXK-RXN, LXULRU5P-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)MANNPISOM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)MANNPISOM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)MANNPDEHYDROG-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)MANNPDEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)ADOMET-DMK-METHYLTRANSFER-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ADOMET-DMK-METHYLTRANSFER-RXN Blocked rxns: 1ADOMET-DMK-METHYLTRANSFER-RXN Essential rxns: 0	Annotated: 5 (46%)S-ADENMETSYN-RXN, ASPARTATEKIN-RXN, O-SUCCHOMOSERLYASE-RXN, HOMSUCTRAN-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN Annot. missing: 6 (54%)RXN-15124, HOMOSERDEHYDROG-RXN, HOMOCYSMETB12-RXN, RXN-15131, HOMOCYSMET-RXN, RXN-15127 Unpredicted: 0 Entire module: 5 (46%)S-ADENMETSYN-RXN, ASPARTATEKIN-RXN, O-SUCCHOMOSERLYASE-RXN, HOMSUCTRAN-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN Blocked rxns: 0 Essential rxns: 5S-ADENMETSYN-RXN, ASPARTATEKIN-RXN, O-SUCCHOMOSERLYASE-RXN, HOMSUCTRAN-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN	Annotated: 4 (50%)GLYOXI-RXN, LACTALDDEHYDROG-RXN, GLYOXII-RXN, RXN0-4281 Annot. missing: 4 (50%)L-LACTDEHYDROGFMN-RXN, RXN-8632, 1.1.1.283-RXN, DLACTDEHYDROGFAD-RXN Unpredicted: 0 Entire module: 4 (50%)GLYOXI-RXN, LACTALDDEHYDROG-RXN, GLYOXII-RXN, RXN0-4281 Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1OXALODECARB-RXN Entire module: 1 (17%)OXALODECARB-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (40%)O-SUCCHOMOSERLYASE-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, HOMSUCTRAN-RXN, ASPARTATEKIN-RXN Annot. missing: 6 (60%)RXN-15124, HOMOSERDEHYDROG-RXN, HOMOCYSMETB12-RXN, RXN-15131, HOMOCYSMET-RXN, RXN-15127 Unpredicted: 0 Entire module: 4 (40%)O-SUCCHOMOSERLYASE-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, HOMSUCTRAN-RXN, ASPARTATEKIN-RXN Blocked rxns: 0 Essential rxns: 4O-SUCCHOMOSERLYASE-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, HOMSUCTRAN-RXN, ASPARTATEKIN-RXN	Unpredicted: 2D-LACTALDEHYDE-DEHYDROGENASE-RXN, RXN-8641 Entire module: 2 (50%)D-LACTALDEHYDE-DEHYDROGENASE-RXN, RXN-8641 Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (67%)RXN-5822, NAD-KIN-RXN Annot. missing: 1 (33%)TRANS-RXN0-277 Unpredicted: 0 Entire module: 2 (67%)RXN-5822, NAD-KIN-RXN Blocked rxns: 0 Essential rxns: 1NAD-KIN-RXN

	lipid IV_A biosynthesis	methylerythritol phosphate pathway I	pentose phosphate pathway (non-oxidative branch)	O-antigen building blocks biosynthesis (E. coli)	superpathway of L-arginine and L-ornithine degradation	superpathway of ornithine degradation	pentose phosphate pathway (oxidative branch) I	citrate lyase activation	superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	phosphopantothenate biosynthesis I
e_coli_core	Annotated: 0 (0%) Annot. missing: 6 (100%)LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, LIPIDXSYNTHESIS-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 9 (100%)RXN0-884, ISPH2-RXN, DXS-RXN, 2.7.1.148-RXN, RXN0-302, RXN-15878, DXPREDISOM-RXN, 2.7.7.60-RXN, IPPISOM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (60%)RIBULP3EPIM-RXN, TRANSALDOL-RXN, 2TRANSKETO-RXN Annot. missing: 2 (40%)RIB5PISOM-RXN, 1TRANSKETO-RXN Unpredicted: 0 Entire module: 3 (60%)RIBULP3EPIM-RXN, TRANSALDOL-RXN, 2TRANSKETO-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (10%)PGLUCISOM-RXN Annot. missing: 9 (90%)DTDPGLUCDEHYDRAT-RXN, 2.3.1.157-RXN, 5.4.2.10-RXN, DTDPGLUCOSEPP-RXN, NAG1P-URIDYLTRANS-RXN, L-GLN-FRUCT-6-P-AMINOTRANS-RXN, DTDPDEHYDRHAMEPIM-RXN, GALPMUT-RXN, DTDPDEHYRHAMREDUCT-RXN Unpredicted: 0 Entire module: 1 (10%)PGLUCISOM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 13 (100%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCSEMIALDDEHYDROG-RXN, AMINOBUTDEHYDROG-RXN, AGMATIN-RXN, RXN0-3901, ARGDECARBOX-RXN, RXN0-3921, ORNDECARBOX-RXN, RXN0-3942, RXN-6423, RXN0-3922, GABATRANSAM-RXN, PUTTRANSAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)AMINOBUTDEHYDROG-RXN, RXN0-3901, RXN0-3942, ORNDECARBOX-RXN, RXN0-3921, RXN-6423, RXN0-3922, PUTTRANSAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)GLU6PDEHYDROG-RXN, RXN-9952, 6PGLUCONOLACT-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)GLU6PDEHYDROG-RXN, RXN-9952, 6PGLUCONOLACT-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)2.7.8.25-RXN, 2.7.7.61-RXN, CITC-RXN, CITRATE-PRO-3S-LYASE-THIOLESTERASE-RXN, 2.3.1.49-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 20 (100%)O-SUCCHOMOSERLYASE-RXN, HOMOSERDEHYDROG-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, DIHYDRODIPICSYN-RXN, RXN-14014, ASPAMINOTRANS-RXN, HOMOCYSMETB12-RXN, HOMOCYSMET-RXN, RXN-15124, ASPARTATEKIN-RXN, DIAMINOPIMDECARB-RXN, SUCCINYLDIAMINOPIMTRANS-RXN, RXN-15127, THRESYN-RXN, HOMOSERKIN-RXN, TETHYDPICSUCC-RXN, HOMSUCTRAN-RXN, SUCCDIAMINOPIMDESUCC-RXN, RXN-15131, DIAMINOPIMEPIM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)2-DEHYDROPANTOATE-REDUCT-RXN, PANTOATE-BETA-ALANINE-LIG-RXN, PANTOTHENATE-KIN-RXN, 3-CH3-2-OXOBUTANOATE-OH-CH3-XFER-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 6 (100%)LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, LIPIDXSYNTHESIS-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 6 (100%)LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, LIPIDXSYNTHESIS-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN Blocked rxns: 0 Essential rxns: 6LIPIDADISACCHARIDESYNTH-RXN, UDPACYLGLCNACDEACETYL-RXN, UDPNACETYLGLUCOSAMACYLTRANS-RXN, TETRAACYLDISACC4KIN-RXN, LIPIDXSYNTHESIS-RXN, UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-RXN	Annotated: 6 (67%)DXS-RXN, 2.7.1.148-RXN, RXN0-302, DXPREDISOM-RXN, 2.7.7.60-RXN, IPPISOM-RXN Annot. missing: 3 (33%)RXN0-884, RXN-15878, ISPH2-RXN Unpredicted: 0 Entire module: 6 (67%)DXS-RXN, 2.7.1.148-RXN, RXN0-302, DXPREDISOM-RXN, 2.7.7.60-RXN, IPPISOM-RXN Blocked rxns: 0 Essential rxns: 5DXS-RXN, 2.7.1.148-RXN, RXN0-302, DXPREDISOM-RXN, 2.7.7.60-RXN	Annotated: 3 (60%)RIBULP3EPIM-RXN, TRANSALDOL-RXN, 2TRANSKETO-RXN Annot. missing: 2 (40%)RIB5PISOM-RXN, 1TRANSKETO-RXN Unpredicted: 0 Entire module: 3 (60%)RIBULP3EPIM-RXN, TRANSALDOL-RXN, 2TRANSKETO-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 10 (100%)DTDPGLUCDEHYDRAT-RXN, 2.3.1.157-RXN, 5.4.2.10-RXN, PGLUCISOM-RXN, DTDPGLUCOSEPP-RXN, NAG1P-URIDYLTRANS-RXN, L-GLN-FRUCT-6-P-AMINOTRANS-RXN, DTDPDEHYDRHAMEPIM-RXN, GALPMUT-RXN, DTDPDEHYRHAMREDUCT-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 10 (100%)DTDPGLUCDEHYDRAT-RXN, 2.3.1.157-RXN, 5.4.2.10-RXN, PGLUCISOM-RXN, DTDPGLUCOSEPP-RXN, NAG1P-URIDYLTRANS-RXN, L-GLN-FRUCT-6-P-AMINOTRANS-RXN, DTDPDEHYDRHAMEPIM-RXN, GALPMUT-RXN, DTDPDEHYRHAMREDUCT-RXN Blocked rxns: 1GALPMUT-RXN Essential rxns: 45.4.2.10-RXN, L-GLN-FRUCT-6-P-AMINOTRANS-RXN, NAG1P-URIDYLTRANS-RXN, 2.3.1.157-RXN	Annotated: 9 (69%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCSEMIALDDEHYDROG-RXN, ARGDECARBOX-RXN, AMINOBUTDEHYDROG-RXN, AGMATIN-RXN, ORNDECARBOX-RXN, RXN0-3942, GABATRANSAM-RXN, PUTTRANSAM-RXN Annot. missing: 4 (31%)RXN0-3901, RXN-6423, RXN0-3922, RXN0-3921 Unpredicted: 0 Entire module: 9 (69%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCSEMIALDDEHYDROG-RXN, ARGDECARBOX-RXN, AMINOBUTDEHYDROG-RXN, AGMATIN-RXN, ORNDECARBOX-RXN, RXN0-3942, GABATRANSAM-RXN, PUTTRANSAM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (50%)RXN0-3942, ORNDECARBOX-RXN, AMINOBUTDEHYDROG-RXN, PUTTRANSAM-RXN Annot. missing: 4 (50%)RXN0-3901, RXN-6423, RXN0-3922, RXN0-3921 Unpredicted: 0 Entire module: 4 (50%)RXN0-3942, ORNDECARBOX-RXN, AMINOBUTDEHYDROG-RXN, PUTTRANSAM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)GLU6PDEHYDROG-RXN, RXN-9952, 6PGLUCONOLACT-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)GLU6PDEHYDROG-RXN, RXN-9952, 6PGLUCONOLACT-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (20%)2.7.8.25-RXN Annot. missing: 4 (80%)2.7.7.61-RXN, CITC-RXN, CITRATE-PRO-3S-LYASE-THIOLESTERASE-RXN, 2.3.1.49-RXN Unpredicted: 0 Entire module: 1 (20%)2.7.8.25-RXN Blocked rxns: 12.7.8.25-RXN Essential rxns: 0	Annotated: 12 (60%)THRESYN-RXN, HOMOSERKIN-RXN, ASPARTATEKIN-RXN, O-SUCCHOMOSERLYASE-RXN, TETHYDPICSUCC-RXN, DIAMINOPIMDECARB-RXN, HOMSUCTRAN-RXN, SUCCDIAMINOPIMDESUCC-RXN, ASPAMINOTRANS-RXN, DIAMINOPIMEPIM-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCINYLDIAMINOPIMTRANS-RXN Annot. missing: 8 (40%)RXN-15124, HOMOSERDEHYDROG-RXN, HOMOCYSMETB12-RXN, RXN-14014, RXN-15131, DIHYDRODIPICSYN-RXN, HOMOCYSMET-RXN, RXN-15127 Unpredicted: 0 Entire module: 12 (60%)THRESYN-RXN, HOMOSERKIN-RXN, ASPARTATEKIN-RXN, O-SUCCHOMOSERLYASE-RXN, TETHYDPICSUCC-RXN, DIAMINOPIMDECARB-RXN, HOMSUCTRAN-RXN, SUCCDIAMINOPIMDESUCC-RXN, ASPAMINOTRANS-RXN, DIAMINOPIMEPIM-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCINYLDIAMINOPIMTRANS-RXN Blocked rxns: 0 Essential rxns: 12THRESYN-RXN, HOMOSERKIN-RXN, ASPARTATEKIN-RXN, O-SUCCHOMOSERLYASE-RXN, TETHYDPICSUCC-RXN, DIAMINOPIMDECARB-RXN, HOMSUCTRAN-RXN, SUCCDIAMINOPIMDESUCC-RXN, ASPAMINOTRANS-RXN, DIAMINOPIMEPIM-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCINYLDIAMINOPIMTRANS-RXN	Annotated: 3 (75%)2-DEHYDROPANTOATE-REDUCT-RXN, PANTOATE-BETA-ALANINE-LIG-RXN, PANTOTHENATE-KIN-RXN Annot. missing: 1 (25%)3-CH3-2-OXOBUTANOATE-OH-CH3-XFER-RXN Unpredicted: 0 Entire module: 3 (75%)2-DEHYDROPANTOATE-REDUCT-RXN, PANTOATE-BETA-ALANINE-LIG-RXN, PANTOTHENATE-KIN-RXN Blocked rxns: 0 Essential rxns: 32-DEHYDROPANTOATE-REDUCT-RXN, PANTOATE-BETA-ALANINE-LIG-RXN, PANTOTHENATE-KIN-RXN

	superpathway of coenzyme A biosynthesis I (bacteria)	pentose phosphate pathway	peptidoglycan biosynthesis I (meso-diaminopimelate containing)	L-phenylalanine biosynthesis I	superpathway of phospholipid biosynthesis I (bacteria)	pyridoxal 5'-phosphate salvage I	superpathway of polyamine biosynthesis I	polyisoprenoid biosynthesis (E. coli)	ppGpp biosynthesis	propanoyl CoA degradation I
e_coli_core	Annotated: 0 (0%) Annot. missing: 9 (100%)ASPDECARBOX-RXN, PANTOTHENATE-KIN-RXN, P-PANTOCYSDECARB-RXN, 2-DEHYDROPANTOATE-REDUCT-RXN, PANTOATE-BETA-ALANINE-LIG-RXN, PANTEPADENYLYLTRAN-RXN, P-PANTOCYSLIG-RXN, DEPHOSPHOCOAKIN-RXN, 3-CH3-2-OXOBUTANOATE-OH-CH3-XFER-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (75%)RXN-9952, 2TRANSKETO-RXN, 6PGLUCONOLACT-RXN, GLU6PDEHYDROG-RXN, RIBULP3EPIM-RXN, TRANSALDOL-RXN Annot. missing: 2 (25%)RIB5PISOM-RXN, 1TRANSKETO-RXN Unpredicted: 0 Entire module: 6 (75%)RXN-9952, 2TRANSKETO-RXN, 6PGLUCONOLACT-RXN, GLU6PDEHYDROG-RXN, RIBULP3EPIM-RXN, TRANSALDOL-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 10 (100%)UDP-NACMURALGLDAPLIG-RXN, UDP-NACMUR-ALA-LIG-RXN, UDP-NACMURALA-GLU-LIG-RXN, DALADALALIG-RXN, PHOSNACMURPENTATRANS-RXN, GLUTRACE-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN, NACGLCTRANS-RXN, UDP-NACMURALGLDAPAALIG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)CHORISMATEMUT-RXN, RXN-10814, PREPHENATEDEHYDRAT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 12 (100%)CDPDIGLYSYN-RXN, CARDIOLIPSYN-RXN, PHOSPHASERDECARB-RXN, GLYC3PDEHYDROGBIOSYN-RXN, 1-ACYLGLYCEROL-3-P-ACYLTRANSFER-RXN, RXN-10462, RXN-1381, PHOSPHASERSYN-RXN, RXN0-7012, RXN-1623, PHOSPHAGLYPSYN-RXN, PGPPHOSPHA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)PYRIDOXKIN-RXN, PYRAMKIN-RXN, PNKIN-RXN, PNPOXI-RXN, PMPOXI-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)LYSDECARBOX-RXN, ARGDECARBOX-RXN, AGMATIN-RXN, RXN0-5217, ORNDECARBOX-RXN, SPERMIDINESYN-RXN, SAMDECARB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)RXN-8999, FPPSYN-RXN, RXN-8992, UNDECAPRENYL-DIPHOSPHATASE-RXN, IPPISOM-RXN, GPPSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)GDPKIN-RXN, GTPPYPHOSKIN-RXN, PPGPPSYN-RXN, PPPGPPHYDRO-RXN, RXN0-6427, GDPPYPHOSKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)PROPIONYL-COA-CARBOXY-RXN, METHYLMALONYL-COA-EPIM-RXN, METHYLMALONYL-COA-MUT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 8 (89%)ASPDECARBOX-RXN, PANTOTHENATE-KIN-RXN, P-PANTOCYSDECARB-RXN, 2-DEHYDROPANTOATE-REDUCT-RXN, PANTOATE-BETA-ALANINE-LIG-RXN, PANTEPADENYLYLTRAN-RXN, P-PANTOCYSLIG-RXN, DEPHOSPHOCOAKIN-RXN Annot. missing: 1 (11%)3-CH3-2-OXOBUTANOATE-OH-CH3-XFER-RXN Unpredicted: 0 Entire module: 8 (89%)ASPDECARBOX-RXN, PANTOTHENATE-KIN-RXN, P-PANTOCYSDECARB-RXN, 2-DEHYDROPANTOATE-REDUCT-RXN, PANTOATE-BETA-ALANINE-LIG-RXN, PANTEPADENYLYLTRAN-RXN, P-PANTOCYSLIG-RXN, DEPHOSPHOCOAKIN-RXN Blocked rxns: 0 Essential rxns: 8ASPDECARBOX-RXN, PANTOTHENATE-KIN-RXN, P-PANTOCYSDECARB-RXN, 2-DEHYDROPANTOATE-REDUCT-RXN, PANTOATE-BETA-ALANINE-LIG-RXN, PANTEPADENYLYLTRAN-RXN, P-PANTOCYSLIG-RXN, DEPHOSPHOCOAKIN-RXN	Annotated: 6 (75%)RXN-9952, 2TRANSKETO-RXN, 6PGLUCONOLACT-RXN, GLU6PDEHYDROG-RXN, RIBULP3EPIM-RXN, TRANSALDOL-RXN Annot. missing: 2 (25%)RIB5PISOM-RXN, 1TRANSKETO-RXN Unpredicted: 0 Entire module: 6 (75%)RXN-9952, 2TRANSKETO-RXN, 6PGLUCONOLACT-RXN, GLU6PDEHYDROG-RXN, RIBULP3EPIM-RXN, TRANSALDOL-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 7 (70%)UDP-NACMUR-ALA-LIG-RXN, UDP-NACMURALA-GLU-LIG-RXN, DALADALALIG-RXN, GLUTRACE-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN, NACGLCTRANS-RXN Annot. missing: 3 (30%)UDP-NACMURALGLDAPLIG-RXN, UDP-NACMURALGLDAPAALIG-RXN, PHOSNACMURPENTATRANS-RXN Unpredicted: 0 Entire module: 7 (70%)UDP-NACMUR-ALA-LIG-RXN, UDP-NACMURALA-GLU-LIG-RXN, DALADALALIG-RXN, GLUTRACE-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN, NACGLCTRANS-RXN Blocked rxns: 0 Essential rxns: 7UDP-NACMUR-ALA-LIG-RXN, UDP-NACMURALA-GLU-LIG-RXN, DALADALALIG-RXN, GLUTRACE-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN, NACGLCTRANS-RXN	Annotated: 3 (100%)CHORISMATEMUT-RXN, RXN-10814, PREPHENATEDEHYDRAT-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)CHORISMATEMUT-RXN, RXN-10814, PREPHENATEDEHYDRAT-RXN Blocked rxns: 0 Essential rxns: 3CHORISMATEMUT-RXN, RXN-10814, PREPHENATEDEHYDRAT-RXN	Annotated: 0 (0%) Annot. missing: 12 (100%)CDPDIGLYSYN-RXN, CARDIOLIPSYN-RXN, PHOSPHASERDECARB-RXN, GLYC3PDEHYDROGBIOSYN-RXN, 1-ACYLGLYCEROL-3-P-ACYLTRANSFER-RXN, RXN-10462, RXN-1381, PHOSPHASERSYN-RXN, RXN0-7012, RXN-1623, PHOSPHAGLYPSYN-RXN, PGPPHOSPHA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (100%)PYRIDOXKIN-RXN, PYRAMKIN-RXN, PNKIN-RXN, PNPOXI-RXN, PMPOXI-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)PYRIDOXKIN-RXN, PYRAMKIN-RXN, PNKIN-RXN, PNPOXI-RXN, PMPOXI-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (86%)LYSDECARBOX-RXN, ARGDECARBOX-RXN, AGMATIN-RXN, ORNDECARBOX-RXN, SPERMIDINESYN-RXN, SAMDECARB-RXN Annot. missing: 1 (14%)RXN0-5217 Unpredicted: 0 Entire module: 6 (86%)LYSDECARBOX-RXN, ARGDECARBOX-RXN, AGMATIN-RXN, ORNDECARBOX-RXN, SPERMIDINESYN-RXN, SAMDECARB-RXN Blocked rxns: 2SPERMIDINESYN-RXN, SAMDECARB-RXN Essential rxns: 0	Annotated: 4 (67%)IPPISOM-RXN, GPPSYN-RXN, FPPSYN-RXN, RXN-8992 Annot. missing: 2 (33%)RXN-8999, UNDECAPRENYL-DIPHOSPHATASE-RXN Unpredicted: 0 Entire module: 4 (67%)IPPISOM-RXN, GPPSYN-RXN, FPPSYN-RXN, RXN-8992 Blocked rxns: 0 Essential rxns: 3GPPSYN-RXN, FPPSYN-RXN, RXN-8992	Annotated: 5 (83%)GDPKIN-RXN, GTPPYPHOSKIN-RXN, PPGPPSYN-RXN, PPPGPPHYDRO-RXN, GDPPYPHOSKIN-RXN Annot. missing: 1 (17%)RXN0-6427 Unpredicted: 0 Entire module: 5 (83%)PPGPPSYN-RXN, GDPKIN-RXN, GTPPYPHOSKIN-RXN, PPPGPPHYDRO-RXN, GDPPYPHOSKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (67%)METHYLMALONYL-COA-EPIM-RXN, METHYLMALONYL-COA-MUT-RXN Annot. missing: 1 (33%)PROPIONYL-COA-CARBOXY-RXN Unpredicted: 0 Entire module: 2 (67%)METHYLMALONYL-COA-EPIM-RXN, METHYLMALONYL-COA-MUT-RXN Blocked rxns: 0 Essential rxns: 0

	L-proline biosynthesis I	superpathway of histidine, purine, and pyrimidine biosynthesis	putrescine degradation I	suberin monomers biosynthesis	CMP-3-deoxy-D-manno-octulosonate biosynthesis	formaldehyde oxidation II (glutathione-dependent)	photorespiration	folate polyglutamylation	glycine betaine degradation I	D-galactose detoxification
e_coli_core	Annotated: 0 (0%) Annot. missing: 4 (100%)PYRROLINECARBREDUCT-RXN, GLUTSEMIALDEHYDROG-RXN, GLUTKIN-RXN, SPONTPRO-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (2%)ADENYL-KIN-RXN Annot. missing: 48 (98%)GDPKIN-RXN, GUANYL-KIN-RXN, OROPRIBTRANS-RXN, RXN0-743, HISTALDEHYD-RXN, PRPPAMIDOTRANS-RXN, GMP-SYN-GLUT-RXN, HISTPRATPHYD-RXN, GLUTAMIDOTRANS-RXN, GARTRANSFORMYL2-RXN, AICARTRANSFORM-RXN, HISTIDPHOS-RXN, RXN0-745, ADENYLOSUCCINATE-SYNTHASE-RXN, RXN0-1401, AICARSYN-RXN, UDPKIN-RXN, RXN0-1402, RXN0-747, HISTCYCLOHYD-RXN, IMIDPHOSDEHYD-RXN, CARBPSYN-RXN, DIHYDROOROTATE-DEHYDROGENASE-RXN, ADPREDUCT-RXN, IMP-DEHYDROG-RXN, PRIBFAICARPISOM-RXN, HISTOLDEHYD-RXN, RXN0-748, DADPKIN-RXN, ATPPHOSPHORIBOSYLTRANS-RXN, PRPPSYN-RXN, RXN0-742, RXN0-746, DGDPKIN-RXN, CTPSYN-RXN, ASPCARBTRANS-RXN, DIHYDROOROT-RXN, GDPREDUCT-RXN, AMPSYN-RXN, FGAMSYN-RXN, SAICARSYN-RXN, HISTAMINOTRANS-RXN, OROTPDECARB-RXN, PPENTOMUT-RXN, RXN-12002, IMPCYCLOHYDROLASE-RXN, GLYRIBONUCSYN-RXN, AIRS-RXN Unpredicted: 0 Entire module: 1 (2%)ADENYL-KIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)AMINOBUTDEHYDROG-RXN, PUTTRANSAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)KDO-8PSYNTH-RXN, RXN-16804, DARAB5PISOM-RXN, KDO-8PPHOSPHAT-RXN, CPM-KDOSYNTH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-2961, RXN-2962, S-FORMYLGLUTATHIONE-HYDROLASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)GLYOHMETRANS-RXN, FORMYLTHFGLUSYNTH-RXN, RXN0-2921, FOLYLPOLYGLUTAMATESYNTH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 3 (75%)GLUTSEMIALDEHYDROG-RXN, GLUTKIN-RXN, SPONTPRO-RXN Annot. missing: 1 (25%)PYRROLINECARBREDUCT-RXN Unpredicted: 0 Entire module: 3 (75%)GLUTSEMIALDEHYDROG-RXN, GLUTKIN-RXN, SPONTPRO-RXN Blocked rxns: 0 Essential rxns: 1SPONTPRO-RXN	Annotated: 37 (76%)GDPKIN-RXN, GUANYL-KIN-RXN, ADENYL-KIN-RXN, OROPRIBTRANS-RXN, RXN0-743, PRPPAMIDOTRANS-RXN, GMP-SYN-GLUT-RXN, HISTPRATPHYD-RXN, GLUTAMIDOTRANS-RXN, GARTRANSFORMYL2-RXN, HISTIDPHOS-RXN, ADENYLOSUCCINATE-SYNTHASE-RXN, RXN0-1401, AICARSYN-RXN, UDPKIN-RXN, RXN0-1402, HISTCYCLOHYD-RXN, IMIDPHOSDEHYD-RXN, CARBPSYN-RXN, DADPKIN-RXN, ADPREDUCT-RXN, IMP-DEHYDROG-RXN, PRIBFAICARPISOM-RXN, ATPPHOSPHORIBOSYLTRANS-RXN, CTPSYN-RXN, DGDPKIN-RXN, ASPCARBTRANS-RXN, DIHYDROOROT-RXN, GDPREDUCT-RXN, AMPSYN-RXN, FGAMSYN-RXN, SAICARSYN-RXN, HISTAMINOTRANS-RXN, OROTPDECARB-RXN, RXN-12002, IMPCYCLOHYDROLASE-RXN, GLYRIBONUCSYN-RXN Annot. missing: 12 (24%)DIHYDROOROTATE-DEHYDROGENASE-RXN, AICARTRANSFORM-RXN, HISTOLDEHYD-RXN, RXN0-748, RXN0-745, PRPPSYN-RXN, HISTALDEHYD-RXN, RXN0-742, RXN0-747, RXN0-746, PPENTOMUT-RXN, AIRS-RXN Unpredicted: 0 Entire module: 35 (76%)GDPKIN-RXN, GUANYL-KIN-RXN, ADENYL-KIN-RXN, OROPRIBTRANS-RXN, RXN0-743, PRPPAMIDOTRANS-RXN, GMP-SYN-GLUT-RXN, HISTPRATPHYD-RXN, GLUTAMIDOTRANS-RXN, GARTRANSFORMYL2-RXN, HISTIDPHOS-RXN, ADENYLOSUCCINATE-SYNTHASE-RXN, AICARSYN-RXN, UDPKIN-RXN, HISTCYCLOHYD-RXN, IMIDPHOSDEHYD-RXN, CARBPSYN-RXN, DADPKIN-RXN, ADPREDUCT-RXN, IMP-DEHYDROG-RXN, PRIBFAICARPISOM-RXN, ATPPHOSPHORIBOSYLTRANS-RXN, CTPSYN-RXN, DGDPKIN-RXN, ASPCARBTRANS-RXN, DIHYDROOROT-RXN, GDPREDUCT-RXN, AMPSYN-RXN, FGAMSYN-RXN, SAICARSYN-RXN, HISTAMINOTRANS-RXN, OROTPDECARB-RXN, RXN-12002, IMPCYCLOHYDROLASE-RXN, GLYRIBONUCSYN-RXN Blocked rxns: 0 Essential rxns: 26GUANYL-KIN-RXN, OROPRIBTRANS-RXN, RXN0-743, PRPPAMIDOTRANS-RXN, GMP-SYN-GLUT-RXN, HISTPRATPHYD-RXN, GLUTAMIDOTRANS-RXN, HISTIDPHOS-RXN, ADENYLOSUCCINATE-SYNTHASE-RXN, AICARSYN-RXN, UDPKIN-RXN, HISTCYCLOHYD-RXN, IMIDPHOSDEHYD-RXN, PRIBFAICARPISOM-RXN, ATPPHOSPHORIBOSYLTRANS-RXN, CTPSYN-RXN, ASPCARBTRANS-RXN, DIHYDROOROT-RXN, AMPSYN-RXN, FGAMSYN-RXN, SAICARSYN-RXN, HISTAMINOTRANS-RXN, OROTPDECARB-RXN, RXN-12002, IMPCYCLOHYDROLASE-RXN, GLYRIBONUCSYN-RXN	Annotated: 2 (100%)AMINOBUTDEHYDROG-RXN, PUTTRANSAM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)AMINOBUTDEHYDROG-RXN, PUTTRANSAM-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1PALMITOYL-COA-HYDROLASE-RXN Entire module: 1 (5%)PALMITOYL-COA-HYDROLASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (60%)CPM-KDOSYNTH-RXN, KDO-8PPHOSPHAT-RXN, DARAB5PISOM-RXN Annot. missing: 2 (40%)KDO-8PSYNTH-RXN, RXN-16804 Unpredicted: 0 Entire module: 3 (60%)CPM-KDOSYNTH-RXN, KDO-8PPHOSPHAT-RXN, DARAB5PISOM-RXN Blocked rxns: 0 Essential rxns: 3CPM-KDOSYNTH-RXN, KDO-8PPHOSPHAT-RXN, DARAB5PISOM-RXN	Annotated: 2 (67%)RXN-2961, S-FORMYLGLUTATHIONE-HYDROLASE-RXN Annot. missing: 1 (33%)RXN-2962 Unpredicted: 0 Entire module: 2 (67%)RXN-2961, S-FORMYLGLUTATHIONE-HYDROLASE-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 3GLY3KIN-RXN, GLYCERATE-DEHYDROGENASE-RXN, GPH-RXN Entire module: 3 (33%)GLY3KIN-RXN, GLYCERATE-DEHYDROGENASE-RXN, GPH-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)GLYOHMETRANS-RXN, FORMYLTHFGLUSYNTH-RXN, RXN0-2921, FOLYLPOLYGLUTAMATESYNTH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1SARCOX-RXN Entire module: 1 (14%)SARCOX-RXN Blocked rxns: 1SARCOX-RXN Essential rxns: 0	Unpredicted: 1GALACTOKIN-RXN Entire module: 2 (67%)GALACTOKIN-RXN, UDPGLUCEPIM-RXN Blocked rxns: 0 Essential rxns: 0

	putrescine biosynthesis I	γ-glutamyl cycle	glutathione-peroxide redox reactions	glutathionylspermidine biosynthesis	glycerol degradation I	S-methyl-5-thio-α-D-ribose 1-phosphate degradation	fatty acid biosynthesis initiation I	putrescine biosynthesis III	arsenate detoxification II (glutaredoxin)	phytate degradation I
e_coli_core	Annotated: 0 (0%) Annot. missing: 2 (100%)ARGDECARBOX-RXN, AGMATIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)GSPSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)GLYCEROL-KIN-RXN, RXN0-5260, RXN-15740 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)2.3.1.180-RXN, RXN0-5055, MALONYL-COA-ACP-TRANSACYL-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ORNDECARBOX-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN-982 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 2 (100%)ARGDECARBOX-RXN, AGMATIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)ARGDECARBOX-RXN, AGMATIN-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1RXN-6622 Entire module: 3 (50%)RXN-6622, GLUTATHIONE-SYN-RXN, GLUTCYSLIG-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1GLUTATHIONE-REDUCT-NADPH-RXN Entire module: 1 (33%)GLUTATHIONE-REDUCT-NADPH-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)GSPSYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GSPSYN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (33%)GLYCEROL-KIN-RXN Annot. missing: 2 (67%)RXN0-5260, RXN-15740 Unpredicted: 0 Entire module: 1 (50%)GLYCEROL-KIN-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2R145-RXN, 5.3.1.23-RXN Entire module: 2 (29%)R145-RXN, 5.3.1.23-RXN Blocked rxns: 2R145-RXN, 5.3.1.23-RXN Essential rxns: 0	Annotated: 2 (67%)2.3.1.180-RXN, MALONYL-COA-ACP-TRANSACYL-RXN Annot. missing: 1 (33%)RXN0-5055 Unpredicted: 0 Entire module: 2 (67%)2.3.1.180-RXN, MALONYL-COA-ACP-TRANSACYL-RXN Blocked rxns: 0 Essential rxns: 1MALONYL-COA-ACP-TRANSACYL-RXN	Annotated: 1 (100%)ORNDECARBOX-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ORNDECARBOX-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN-982 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1RXN0-5408 Entire module: 1 (7%)RXN0-5408 Blocked rxns: 1RXN0-5408 Essential rxns: 0

	biotin biosynthesis II	purine nucleotides degradation I (plants)	NAD/NADH phosphorylation and dephosphorylation	2-oxoglutarate decarboxylation to succinyl-CoA	trans, trans-farnesyl diphosphate biosynthesis	β-alanine biosynthesis III	2-hydroxypenta-2,4-dienoate degradation	tetrapyrrole biosynthesis II (from glycine)	siroheme biosynthesis	superpathway of pentose and pentitol degradation
e_coli_core	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1PYRNUTRANSHYDROGEN-RXN Entire module: 1 (17%)PYRNUTRANSHYDROGEN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-1147, RXN-7716, 2OXOGLUTDECARB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)IPPISOM-RXN, GPPSYN-RXN, FPPSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ASPDECARBOX-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (33%)ACETALD-DEHYDROG-RXN Annot. missing: 2 (67%)2-OXOPENT-4-ENOATE-HYDRATASE-RXN, MHPELY-RXN Unpredicted: 0 Entire module: 1 (33%)ACETALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)SIROHEME-FERROCHELAT-RXN, DIMETHUROPORDEHYDROG-RXN, RXN-8675, UROPORIIIMETHYLTRANSA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 1 (2%)MALSYN-RXN Blocked rxns: 0 Essential rxns: 0
iAF1260	Unpredicted: 17KAPSYN-RXN Entire module: 2 (40%)7KAPSYN-RXN, DETHIOBIOTIN-SYN-RXN Blocked rxns: 27KAPSYN-RXN, DETHIOBIOTIN-SYN-RXN Essential rxns: 0	Unpredicted: 4RXN0-363, INOSINE-NUCLEOSIDASE-RXN, RXN-7607, XMPXAN-RXN Entire module: 9 (75%)RXN-7609, IMP-DEHYDROG-RXN, INOSINE-NUCLEOSIDASE-RXN, RXN-7682, RXN0-363, XMPXAN-RXN, RXN-7607, GUANINE-DEAMINASE-RXN, RXN0-901 Blocked rxns: 2XMPXAN-RXN, RXN-7607 Essential rxns: 0	Unpredicted: 1PYRNUTRANSHYDROGEN-RXN Entire module: 3 (50%)PYRNUTRANSHYDROGEN-RXN, RXN-5822, NAD-KIN-RXN Blocked rxns: 0 Essential rxns: 1NAD-KIN-RXN	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-1147, RXN-7716, 2OXOGLUTDECARB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)IPPISOM-RXN, GPPSYN-RXN, FPPSYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)IPPISOM-RXN, GPPSYN-RXN, FPPSYN-RXN Blocked rxns: 0 Essential rxns: 2GPPSYN-RXN, FPPSYN-RXN	Annotated: 1 (100%)ASPDECARBOX-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ASPDECARBOX-RXN Blocked rxns: 0 Essential rxns: 1ASPDECARBOX-RXN	Annotated: 2 (67%)ACETALD-DEHYDROG-RXN, MHPELY-RXN Annot. missing: 1 (33%)2-OXOPENT-4-ENOATE-HYDRATASE-RXN Unpredicted: 0 Entire module: 2 (67%)ACETALD-DEHYDROG-RXN, MHPELY-RXN Blocked rxns: 1MHPELY-RXN Essential rxns: 0	Unpredicted: 3UROGENIIISYN-RXN, OHMETHYLBILANESYN-RXN, PORPHOBILSYNTH-RXN Entire module: 3 (75%)UROGENIIISYN-RXN, OHMETHYLBILANESYN-RXN, PORPHOBILSYNTH-RXN Blocked rxns: 0 Essential rxns: 3UROGENIIISYN-RXN, OHMETHYLBILANESYN-RXN, PORPHOBILSYNTH-RXN	Annotated: 2 (50%)SIROHEME-FERROCHELAT-RXN, DIMETHUROPORDEHYDROG-RXN Annot. missing: 2 (50%)RXN-8675, UROPORIIIMETHYLTRANSA-RXN Unpredicted: 0 Entire module: 2 (67%)SIROHEME-FERROCHELAT-RXN, DIMETHUROPORDEHYDROG-RXN Blocked rxns: 0 Essential rxns: 2SIROHEME-FERROCHELAT-RXN, DIMETHUROPORDEHYDROG-RXN	Unpredicted: 1GLYCOLATE-REDUCTASE-RXN Entire module: 9 (22%)GLYCOLATE-REDUCTASE-RXN, GLYCOLALD-DEHYDROG-RXN, XYLISOM-RXN, RXN0-5116, RIBULPEPIM-RXN, LXULRU5P-RXN, LYXK-RXN, MALSYN-RXN, XYLULOKIN-RXN Blocked rxns: 1RXN0-5116 Essential rxns: 1GLYCOLALD-DEHYDROG-RXN

	superpathway of L-methionine salvage and degradation	L-cysteine degradation III	sulfate activation for sulfonation	thiosulfate disproportionation IV (rhodanese)	methylglyoxal degradation I	L-threonine degradation IV	L-threonine degradation I	S-methyl-L-methionine cycle	methylglyoxal degradation III	methylglyoxal degradation IV
e_coli_core	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)ADENYLYLSULFKIN-RXN, SULFATE-ADENYLYLTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)THIOSULFATE-SULFURTRANSFERASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)GLYOXI-RXN, DLACTDEHYDROGFAD-RXN, GLYOXII-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (50%)ACETALD-DEHYDROG-RXN Annot. missing: 1 (50%)THREONINE-ALDOLASE-RXN Unpredicted: 0 Entire module: 1 (50%)ACETALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)RXN-15121, RXN-15122, KETOBUTFORMLY-RXN, PROPKIN-RXN, RXN-15123, PTAALT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN-8632, RXN0-4281 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)L-LACTDEHYDROGFMN-RXN, LACTALDDEHYDROG-RXN, 1.1.1.283-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Unpredicted: 1MMUM-RXN Entire module: 4 (22%)METHYLMALONYL-COA-EPIM-RXN, S-ADENMETSYN-RXN, MMUM-RXN, METHYLMALONYL-COA-MUT-RXN Blocked rxns: 1MMUM-RXN Essential rxns: 1S-ADENMETSYN-RXN	Unpredicted: 1MERCAPYSTRANS-RXN Entire module: 1 (50%)MERCAPYSTRANS-RXN Blocked rxns: 1MERCAPYSTRANS-RXN Essential rxns: 0	Annotated: 1 (50%)ADENYLYLSULFKIN-RXN Annot. missing: 1 (50%)SULFATE-ADENYLYLTRANS-RXN Unpredicted: 0 Entire module: 1 (50%)ADENYLYLSULFKIN-RXN Blocked rxns: 0 Essential rxns: 1ADENYLYLSULFKIN-RXN	Annotated: 1 (100%)THIOSULFATE-SULFURTRANSFERASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)THIOSULFATE-SULFURTRANSFERASE-RXN Blocked rxns: 1THIOSULFATE-SULFURTRANSFERASE-RXN Essential rxns: 0	Annotated: 2 (67%)GLYOXI-RXN, GLYOXII-RXN Annot. missing: 1 (33%)DLACTDEHYDROGFAD-RXN Unpredicted: 0 Entire module: 2 (67%)GLYOXI-RXN, GLYOXII-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)ACETALD-DEHYDROG-RXN, THREONINE-ALDOLASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)ACETALD-DEHYDROG-RXN, THREONINE-ALDOLASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (33%)KETOBUTFORMLY-RXN, PTAALT-RXN Annot. missing: 4 (67%)PROPKIN-RXN, RXN-15121, RXN-15122, RXN-15123 Unpredicted: 0 Entire module: 2 (33%)KETOBUTFORMLY-RXN, PTAALT-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1MMUM-RXN Entire module: 1 (50%)MMUM-RXN Blocked rxns: 1MMUM-RXN Essential rxns: 0	Annotated: 1 (50%)RXN0-4281 Annot. missing: 1 (50%)RXN-8632 Unpredicted: 0 Entire module: 1 (50%)RXN0-4281 Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (33%)LACTALDDEHYDROG-RXN Annot. missing: 2 (67%)L-LACTDEHYDROGFMN-RXN, 1.1.1.283-RXN Unpredicted: 0 Entire module: 1 (33%)LACTALDDEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0

	glycolysis II (from fructose 6-phosphate)	adenosylcobalamin biosynthesis from adenosylcobinamide-GDP I	GDP-mannose biosynthesis	CDP-diacylglycerol biosynthesis I	cardiolipin biosynthesis I	phosphatidylethanolamine biosynthesis I	UMP biosynthesis I	allantoin degradation to glyoxylate II	allantoin degradation to ureidoglycolate II (ammonia producing)	allantoin degradation to glyoxylate III
e_coli_core	Annotated: 11 (100%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 11 (100%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 5RXN-15513, PHOSGLYPHOS-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, 3PGAREARR-RXN	Annotated: 0 (0%) Annot. missing: 3 (100%)COBALAMIN5PSYN-RXN, DMBPPRIBOSYLTRANS-RXN, RXN-8770 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (25%)PGLUCISOM-RXN Annot. missing: 3 (75%)2.7.7.13-RXN, PHOSMANMUT-RXN, MANNPISOM-RXN Unpredicted: 0 Entire module: 1 (25%)PGLUCISOM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)CDPDIGLYSYN-RXN, RXN-1381, RXN-1623, GLYC3PDEHYDROGBIOSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)CARDIOLIPSYN-RXN, PGPPHOSPHA-RXN, PHOSPHAGLYPSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PHOSPHASERSYN-RXN, PHOSPHASERDECARB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)CARBPSYN-RXN, DIHYDROOROTATE-DEHYDROGENASE-RXN, ASPCARBTRANS-RXN, DIHYDROOROT-RXN, OROPRIBTRANS-RXN, OROTPDECARB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)ALLANTOINASE-RXN, ALLANTOATE-DEIMINASE-RXN, URUR-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)ALLANTOINASE-RXN, UREIDOGLYCOLATE-LYASE-RXN, URUR-RXN, ALLANTOATE-DEIMINASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 11 (100%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 11 (100%)PEPSYNTH-RXN, RXN-15513, F16BDEPHOS-RXN, PHOSGLYPHOS-RXN, 6PFRUCTPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (33%)DMBPPRIBOSYLTRANS-RXN Annot. missing: 2 (67%)COBALAMIN5PSYN-RXN, RXN-8770 Unpredicted: 0 Entire module: 1 (33%)DMBPPRIBOSYLTRANS-RXN Blocked rxns: 1DMBPPRIBOSYLTRANS-RXN Essential rxns: 0	Annotated: 3 (75%)PHOSMANMUT-RXN, PGLUCISOM-RXN, MANNPISOM-RXN Annot. missing: 1 (25%)2.7.7.13-RXN Unpredicted: 0 Entire module: 3 (75%)PHOSMANMUT-RXN, PGLUCISOM-RXN, MANNPISOM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)CDPDIGLYSYN-RXN, RXN-1381, RXN-1623, GLYC3PDEHYDROGBIOSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)CARDIOLIPSYN-RXN, PGPPHOSPHA-RXN, PHOSPHAGLYPSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PHOSPHASERSYN-RXN, PHOSPHASERDECARB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (83%)CARBPSYN-RXN, DIHYDROOROT-RXN, ASPCARBTRANS-RXN, OROPRIBTRANS-RXN, OROTPDECARB-RXN Annot. missing: 1 (17%)DIHYDROOROTATE-DEHYDROGENASE-RXN Unpredicted: 0 Entire module: 5 (83%)CARBPSYN-RXN, DIHYDROOROT-RXN, ASPCARBTRANS-RXN, OROPRIBTRANS-RXN, OROTPDECARB-RXN Blocked rxns: 0 Essential rxns: 4ASPCARBTRANS-RXN, DIHYDROOROT-RXN, OROTPDECARB-RXN, OROPRIBTRANS-RXN	Unpredicted: 1UREIDOGLYCOLATE-HYDROLASE-RXN Entire module: 1 (25%)UREIDOGLYCOLATE-HYDROLASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)ALLANTOINASE-RXN, ALLANTOATE-DEIMINASE-RXN, URUR-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)ALLANTOINASE-RXN, UREIDOGLYCOLATE-LYASE-RXN, URUR-RXN, ALLANTOATE-DEIMINASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	4-hydroxybenzoate biosynthesis II (microbes)	octaprenyl diphosphate biosynthesis	di-trans,poly-cis-undecaprenyl phosphate biosynthesis	3-hydroxypropanoate/4-hydroxybutanate cycle	all trans undecaprenyl diphosphate biosynthesis	2-carboxy-1,4-naphthoquinol biosynthesis	superpathway of menaquinol-8 biosynthesis I	demethylmenaquinol-8 biosynthesis I	superpathway of menaquinol-11 biosynthesis	2,3-dihydroxybenzoate biosynthesis
e_coli_core	Annotated: 0 (0%) Annot. missing: 1 (100%)CHORPYRLY-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN-8992 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN-8999, UNDECAPRENYL-DIPHOSPHATASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)ISOCHORSYN-RXN, O-SUCCINYLBENZOATE-COA-SYN-RXN, NAPHTHOATE-SYN-RXN, RXN-9310, RXN-9311, O-SUCCINYLBENZOATE-COA-LIG-RXN, 2.5.1.64-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 10 (100%)ISOCHORSYN-RXN, O-SUCCINYLBENZOATE-COA-SYN-RXN, NAPHTHOATE-SYN-RXN, RXN-8992, RXN-9310, RXN-9311, O-SUCCINYLBENZOATE-COA-LIG-RXN, 2.5.1.64-RXN, ADOMET-DMK-METHYLTRANSFER-RXN, DMK-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)DMK-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)ISOCHORMAT-RXN, DHBDEHYD-RXN, ISOCHORSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 1 (100%)CHORPYRLY-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)CHORPYRLY-RXN Blocked rxns: 0 Essential rxns: 1CHORPYRLY-RXN	Annotated: 1 (100%)RXN-8992 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)RXN-8992 Blocked rxns: 0 Essential rxns: 1RXN-8992	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN-8999, UNDECAPRENYL-DIPHOSPHATASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2RXN-11662, RXN-11667 Entire module: 5 (28%)RXN-11662, RXN-11667, ACETYL-COA-ACETYLTRANSFER-RXN, METHYLMALONYL-COA-EPIM-RXN, METHYLMALONYL-COA-MUT-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1RXN-9138 Entire module: 1 (100%)RXN-9138 Blocked rxns: 0 Essential rxns: 1RXN-9138	Annotated: 3 (43%)O-SUCCINYLBENZOATE-COA-SYN-RXN, O-SUCCINYLBENZOATE-COA-LIG-RXN, ISOCHORSYN-RXN Annot. missing: 4 (57%)RXN-9310, NAPHTHOATE-SYN-RXN, RXN-9311, 2.5.1.64-RXN Unpredicted: 0 Entire module: 3 (43%)O-SUCCINYLBENZOATE-COA-SYN-RXN, O-SUCCINYLBENZOATE-COA-LIG-RXN, ISOCHORSYN-RXN Blocked rxns: 2O-SUCCINYLBENZOATE-COA-SYN-RXN, O-SUCCINYLBENZOATE-COA-LIG-RXN Essential rxns: 0	Annotated: 6 (60%)ISOCHORSYN-RXN, O-SUCCINYLBENZOATE-COA-SYN-RXN, RXN-8992, O-SUCCINYLBENZOATE-COA-LIG-RXN, ADOMET-DMK-METHYLTRANSFER-RXN, DMK-RXN Annot. missing: 4 (40%)RXN-9310, NAPHTHOATE-SYN-RXN, RXN-9311, 2.5.1.64-RXN Unpredicted: 0 Entire module: 6 (60%)ISOCHORSYN-RXN, O-SUCCINYLBENZOATE-COA-SYN-RXN, RXN-8992, O-SUCCINYLBENZOATE-COA-LIG-RXN, ADOMET-DMK-METHYLTRANSFER-RXN, DMK-RXN Blocked rxns: 4DMK-RXN, O-SUCCINYLBENZOATE-COA-SYN-RXN, O-SUCCINYLBENZOATE-COA-LIG-RXN, ADOMET-DMK-METHYLTRANSFER-RXN Essential rxns: 1RXN-8992	Annotated: 1 (100%)DMK-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)DMK-RXN Blocked rxns: 1DMK-RXN Essential rxns: 0	Unpredicted: 1RXN-9138 Entire module: 4 (40%)O-SUCCINYLBENZOATE-COA-SYN-RXN, RXN-9138, O-SUCCINYLBENZOATE-COA-LIG-RXN, ISOCHORSYN-RXN Blocked rxns: 2O-SUCCINYLBENZOATE-COA-SYN-RXN, O-SUCCINYLBENZOATE-COA-LIG-RXN Essential rxns: 1RXN-9138	Annotated: 3 (100%)ISOCHORMAT-RXN, DHBDEHYD-RXN, ISOCHORSYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)ISOCHORMAT-RXN, DHBDEHYD-RXN, ISOCHORSYN-RXN Blocked rxns: 0 Essential rxns: 0

	superpathway of b heme biosynthesis from glycine	guanylyl molybdenum cofactor biosynthesis	fatty acid biosynthesis initiation III	fatty acid biosynthesis initiation II	palmitate biosynthesis II (bacteria and plants)	cis-vaccenate biosynthesis	acyl carrier protein metabolism	pseudouridine degradation	citrate degradation	dimethyl sulfide degradation II (oxidation)
e_coli_core	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN0-262 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)MALONYL-ACPDECARBOX-RXN, 3-OXOACYL-ACP-SYNTH-BASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)3-OXOACYL-ACP-SYNTH-BASE-RXN, ACP-S-ACETYLTRANSFER-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 27 (100%)RXN-9540, RXN-9661, RXN-9663, RXN-9660, RXN-9659, RXN-9662, 4.2.1.61-RXN, RXN-9516, RXN-9528, RXN-9533, RXN-9520, RXN-9531, RXN-9524, 4.2.1.59-RXN, RXN-9539, RXN-9527, RXN-9536, RXN-9655, RXN-9523, RXN-9514, RXN-9657, 4.2.1.58-RXN, RXN-9532, RXN-9658, RXN-9518, RXN-9535, RXN-9537 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)2.3.1.179-RXN, RXN-9556, RXN-9557, RXN-9555, RXN-9558 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)3.1.4.14-RXN, HOLO-ACP-SYNTH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PSEUDOURIDINE-KINASE-RXN, RXN0-5398 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)CITTRANS-RXN, CITRYLY-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Unpredicted: 3UROGENIIISYN-RXN, OHMETHYLBILANESYN-RXN, PORPHOBILSYNTH-RXN Entire module: 6 (75%)OHMETHYLBILANESYN-RXN, UROGENIIISYN-RXN, PORPHOBILSYNTH-RXN, RXN0-1461, UROGENDECARBOX-RXN, PROTOHEMEFERROCHELAT-RXN Blocked rxns: 0 Essential rxns: 5OHMETHYLBILANESYN-RXN, UROGENIIISYN-RXN, PORPHOBILSYNTH-RXN, UROGENDECARBOX-RXN, PROTOHEMEFERROCHELAT-RXN	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN0-262 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)MALONYL-ACPDECARBOX-RXN, 3-OXOACYL-ACP-SYNTH-BASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)MALONYL-ACPDECARBOX-RXN, 3-OXOACYL-ACP-SYNTH-BASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)3-OXOACYL-ACP-SYNTH-BASE-RXN, ACP-S-ACETYLTRANSFER-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)3-OXOACYL-ACP-SYNTH-BASE-RXN, ACP-S-ACETYLTRANSFER-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 11 (41%)RXN-9540, RXN-9524, 4.2.1.59-RXN, 4.2.1.61-RXN, RXN-9532, RXN-9528, RXN-9518, RXN-9537, RXN-9533, RXN-9536, RXN-9520 Annot. missing: 16 (59%)RXN-9514, RXN-9661, RXN-9659, RXN-9657, RXN-9662, RXN-9539, RXN-9527, 4.2.1.58-RXN, RXN-9516, RXN-9658, RXN-9663, RXN-9655, RXN-9535, RXN-9523, RXN-9531, RXN-9660 Unpredicted: 0 Entire module: 11 (36%)RXN-9540, RXN-9524, 4.2.1.59-RXN, 4.2.1.61-RXN, RXN-9532, RXN-9528, RXN-9518, RXN-9537, RXN-9533, RXN-9536, RXN-9520 Blocked rxns: 0 Essential rxns: 6RXN-9524, 4.2.1.59-RXN, RXN-9528, RXN-9518, RXN-9536, RXN-9520	Annotated: 0 (0%) Annot. missing: 5 (100%)2.3.1.179-RXN, RXN-9556, RXN-9557, RXN-9555, RXN-9558 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)3.1.4.14-RXN, HOLO-ACP-SYNTH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PSEUDOURIDINE-KINASE-RXN, RXN0-5398 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)CITTRANS-RXN, CITRYLY-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1RXN-18086 Entire module: 1 (20%)RXN-18086 Blocked rxns: 0 Essential rxns: 0

	superpathway of phenylethylamine degradation	superpathway of mycolate biosynthesis	spermine and spermidine degradation I	5-aminoimidazole ribonucleotide biosynthesis I	5-aminoimidazole ribonucleotide biosynthesis II	inosine-5'-phosphate biosynthesis I	superpathway of guanosine nucleotides de novo biosynthesis II	superpathway of adenosine nucleotides de novo biosynthesis II	superpathway of CMP-sialic acids biosynthesis	6-hydroxymethyl-dihydropterin diphosphate biosynthesis I
e_coli_core	Annotated: 0 (0%) Annot. missing: 12 (100%)AMINEPHEN-RXN, RXN0-2042, RXNMETA-12672, RXN-10819, RXN-3641, RXN0-2044, RXN-2425, RXN0-6510, RXN0-6512, TRANS-RXN0-284, PHENDEHYD-RXN, RXNMETA-12671 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)FGAMSYN-RXN, AIRS-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN, GART-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)GARTRANSFORMYL2-RXN, FGAMSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN, AIRS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)AICARTRANSFORM-RXN, IMPCYCLOHYDROLASE-RXN, RXN0-743, AICARSYN-RXN, RXN0-742, SAICARSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)GDPKIN-RXN, GDPREDUCT-RXN, GUANYL-KIN-RXN, IMP-DEHYDROG-RXN, RXN0-748, RXN0-746, DGDPKIN-RXN, GMP-SYN-GLUT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (14%)ADENYL-KIN-RXN Annot. missing: 6 (86%)DADPKIN-RXN, ADPREDUCT-RXN, AMPSYN-RXN, RXN0-745, ADENYLOSUCCINATE-SYNTHASE-RXN, RXN0-747 Unpredicted: 0 Entire module: 1 (14%)ADENYL-KIN-RXN Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, H2NEOPTERINALDOL-RXN, H2PTERIDINEPYROPHOSPHOKIN-RXN, GTP-CYCLOHYDRO-I-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 3 (25%)PHENDEHYD-RXN, AMINEPHEN-RXN, RXN-10819 Annot. missing: 9 (75%)RXN0-2042, RXNMETA-12672, RXN-3641, RXN-2425, RXN0-2044, RXN0-6510, RXN0-6512, TRANS-RXN0-284, RXNMETA-12671 Unpredicted: 0 Entire module: 3 (27%)PHENDEHYD-RXN, AMINEPHEN-RXN, RXN-10819 Blocked rxns: 2PHENDEHYD-RXN, RXN-10819 Essential rxns: 0	Unpredicted: 1RXN-9634 Entire module: 14 (6%)RXN-9524, RXN-9540, 4.2.1.59-RXN, 4.2.1.61-RXN, 2.3.1.180-RXN, RXN-9634, RXN-9532, MALONYL-COA-ACP-TRANSACYL-RXN, RXN-9528, RXN-9518, RXN-9537, RXN-9533, RXN-9536, RXN-9520 Blocked rxns: 0 Essential rxns: 7RXN-9524, 4.2.1.59-RXN, MALONYL-COA-ACP-TRANSACYL-RXN, RXN-9528, RXN-9518, RXN-9536, RXN-9520	Unpredicted: 1SPERMACTRAN-RXN Entire module: 1 (20%)SPERMACTRAN-RXN Blocked rxns: 1SPERMACTRAN-RXN Essential rxns: 0	Annotated: 3 (60%)FGAMSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN Annot. missing: 2 (40%)GART-RXN, AIRS-RXN Unpredicted: 0 Entire module: 3 (60%)FGAMSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN Blocked rxns: 0 Essential rxns: 3FGAMSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN	Annotated: 4 (80%)FGAMSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN, GARTRANSFORMYL2-RXN Annot. missing: 1 (20%)AIRS-RXN Unpredicted: 0 Entire module: 4 (80%)FGAMSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN, GARTRANSFORMYL2-RXN Blocked rxns: 0 Essential rxns: 3FGAMSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN	Annotated: 4 (67%)IMPCYCLOHYDROLASE-RXN, SAICARSYN-RXN, AICARSYN-RXN, RXN0-743 Annot. missing: 2 (33%)AICARTRANSFORM-RXN, RXN0-742 Unpredicted: 0 Entire module: 4 (67%)IMPCYCLOHYDROLASE-RXN, SAICARSYN-RXN, AICARSYN-RXN, RXN0-743 Blocked rxns: 0 Essential rxns: 4IMPCYCLOHYDROLASE-RXN, SAICARSYN-RXN, AICARSYN-RXN, RXN0-743	Annotated: 6 (75%)GDPKIN-RXN, GDPREDUCT-RXN, GUANYL-KIN-RXN, IMP-DEHYDROG-RXN, DGDPKIN-RXN, GMP-SYN-GLUT-RXN Annot. missing: 2 (25%)RXN0-746, RXN0-748 Unpredicted: 0 Entire module: 6 (75%)GDPKIN-RXN, GDPREDUCT-RXN, GUANYL-KIN-RXN, IMP-DEHYDROG-RXN, DGDPKIN-RXN, GMP-SYN-GLUT-RXN Blocked rxns: 0 Essential rxns: 2GMP-SYN-GLUT-RXN, GUANYL-KIN-RXN	Annotated: 5 (71%)DADPKIN-RXN, ADPREDUCT-RXN, ADENYL-KIN-RXN, AMPSYN-RXN, ADENYLOSUCCINATE-SYNTHASE-RXN Annot. missing: 2 (29%)RXN0-745, RXN0-747 Unpredicted: 0 Entire module: 5 (71%)DADPKIN-RXN, ADPREDUCT-RXN, ADENYL-KIN-RXN, AMPSYN-RXN, ADENYLOSUCCINATE-SYNTHASE-RXN Blocked rxns: 0 Essential rxns: 2ADENYLOSUCCINATE-SYNTHASE-RXN, AMPSYN-RXN	Unpredicted: 1MANNKIN-RXN Entire module: 2 (13%)MANNKIN-RXN, NANK-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (100%)DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, H2NEOPTERINALDOL-RXN, H2PTERIDINEPYROPHOSPHOKIN-RXN, GTP-CYCLOHYDRO-I-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, H2NEOPTERINALDOL-RXN, H2PTERIDINEPYROPHOSPHOKIN-RXN, GTP-CYCLOHYDRO-I-RXN Blocked rxns: 0 Essential rxns: 5DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, H2NEOPTERINALDOL-RXN, H2PTERIDINEPYROPHOSPHOKIN-RXN, GTP-CYCLOHYDRO-I-RXN

	S-adenosyl-L-methionine cycle I	autoinducer AI-2 biosynthesis I	chorismate biosynthesis from 3-dehydroquinate	3-dehydroquinate biosynthesis I	adenosylcobalamin salvage from cobalamin	superpathway of 5-aminoimidazole ribonucleotide biosynthesis	palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)	superpathway of unsaturated fatty acids biosynthesis (E. coli)	L-cysteine biosynthesis IV (fungi)	L-phenylalanine degradation IV (mammalian, via side chain)
e_coli_core	Annotated: 0 (0%) Annot. missing: 5 (100%)ADENOSYLHOMOCYSTEINE-NUCLEOSIDASE-RXN, S-ADENMETSYN-RXN, RIBOSYLHOMOCYSTEINASE-RXN, RXN-7605, HOMOCYSMET-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)ADENOSYLHOMOCYSTEINE-NUCLEOSIDASE-RXN, RXN-10017, RXN-10015, RIBOSYLHOMOCYSTEINASE-RXN, RXN-7605 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)2.5.1.19-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, SHIKIMATE-KINASE-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)3-DEHYDROQUINATE-SYNTHASE-RXN, DAHPSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)RXN-19340, COBALADENOSYLTRANS-RXN, RXN-19334, RXN-19341, RXN-19366 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)GARTRANSFORMYL2-RXN, FGAMSYN-RXN, AIRS-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN, GART-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 9 (100%)RXN-10656, RXN-10654, RXN-10661, RXN-9550, RXN-10659, RXN-10657, RXN-10658, RXN-10660, RXN-10655 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 20 (100%)RXN-10656, 2.3.1.179-RXN, RXN-9556, RXN0-2144, RXN-10659, RXN-10660, RXN-10654, RXN-10658, RXN-10661, RXN0-2141, RXN0-2145, RXN-9655, RXN-10657, RXN-9555, RXN-10655, RXN-9558, RXN0-2142, RXN-9550, RXN-9557, 5.3.3.14-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 3 (60%)ADENOSYLHOMOCYSTEINE-NUCLEOSIDASE-RXN, RIBOSYLHOMOCYSTEINASE-RXN, S-ADENMETSYN-RXN Annot. missing: 2 (40%)RXN-7605, HOMOCYSMET-RXN Unpredicted: 0 Entire module: 3 (60%)ADENOSYLHOMOCYSTEINE-NUCLEOSIDASE-RXN, RIBOSYLHOMOCYSTEINASE-RXN, S-ADENMETSYN-RXN Blocked rxns: 0 Essential rxns: 3ADENOSYLHOMOCYSTEINE-NUCLEOSIDASE-RXN, RIBOSYLHOMOCYSTEINASE-RXN, S-ADENMETSYN-RXN	Annotated: 2 (40%)ADENOSYLHOMOCYSTEINE-NUCLEOSIDASE-RXN, RIBOSYLHOMOCYSTEINASE-RXN Annot. missing: 3 (60%)RXN-10017, RXN-10015, RXN-7605 Unpredicted: 0 Entire module: 2 (40%)ADENOSYLHOMOCYSTEINE-NUCLEOSIDASE-RXN, RIBOSYLHOMOCYSTEINASE-RXN Blocked rxns: 0 Essential rxns: 2ADENOSYLHOMOCYSTEINE-NUCLEOSIDASE-RXN, RIBOSYLHOMOCYSTEINASE-RXN	Annotated: 5 (100%)2.5.1.19-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, SHIKIMATE-KINASE-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)2.5.1.19-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, SHIKIMATE-KINASE-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN Blocked rxns: 0 Essential rxns: 52.5.1.19-RXN, 3-DEHYDROQUINATE-DEHYDRATASE-RXN, SHIKIMATE-KINASE-RXN, SHIKIMATE-5-DEHYDROGENASE-RXN, CHORISMATE-SYNTHASE-RXN	Annotated: 2 (100%)3-DEHYDROQUINATE-SYNTHASE-RXN, DAHPSYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)3-DEHYDROQUINATE-SYNTHASE-RXN, DAHPSYN-RXN Blocked rxns: 0 Essential rxns: 23-DEHYDROQUINATE-SYNTHASE-RXN, DAHPSYN-RXN	Annotated: 1 (20%)COBALADENOSYLTRANS-RXN Annot. missing: 4 (80%)RXN-19366, RXN-19340, RXN-19334, RXN-19341 Unpredicted: 0 Entire module: 1 (20%)COBALADENOSYLTRANS-RXN Blocked rxns: 1COBALADENOSYLTRANS-RXN Essential rxns: 0	Annotated: 4 (67%)FGAMSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN, GARTRANSFORMYL2-RXN Annot. missing: 2 (33%)AIRS-RXN, GART-RXN Unpredicted: 0 Entire module: 4 (67%)FGAMSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN, GARTRANSFORMYL2-RXN Blocked rxns: 0 Essential rxns: 3FGAMSYN-RXN, PRPPAMIDOTRANS-RXN, GLYRIBONUCSYN-RXN	Annotated: 0 (0%) Annot. missing: 9 (100%)RXN-10656, RXN-10654, RXN-10661, RXN-9550, RXN-10659, RXN-10657, RXN-10658, RXN-10660, RXN-10655 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 20 (100%)RXN-10656, 2.3.1.179-RXN, RXN-9556, RXN0-2144, RXN-10659, RXN-10660, RXN-10654, RXN-10658, RXN-10661, RXN0-2141, RXN0-2145, RXN-9655, RXN-10657, RXN-9555, RXN-10655, RXN-9558, RXN0-2142, RXN-9550, RXN-9557, 5.3.3.14-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1CYSTATHIONINE-BETA-LYASE-RXN Entire module: 2 (33%)CYSTATHIONINE-BETA-LYASE-RXN, O-SUCCHOMOSERLYASE-RXN Blocked rxns: 0 Essential rxns: 2CYSTATHIONINE-BETA-LYASE-RXN, O-SUCCHOMOSERLYASE-RXN	Unpredicted: 1RXN-10817 Entire module: 4 (44%)PHENDEHYD-RXN, RXN-10817, RXN-10819, RXN-10814 Blocked rxns: 2PHENDEHYD-RXN, RXN-10819 Essential rxns: 1RXN-10814

	syringate degradation	purine nucleotides degradation II (aerobic)	UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)	vancomycin resistance I	vancomycin resistance II	cytidylyl molybdenum cofactor biosynthesis	N-acetylglucosamine degradation II	8-amino-7-oxononanoate biosynthesis I	4-aminobutanoate degradation I	4-aminobutanoate degradation II
e_coli_core	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)UDP-NACMURALGLDAPLIG-RXN, UDP-NACMURALA-GLU-LIG-RXN, DALADALALIG-RXN, GLUTRACE-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN, UDP-NACMUR-ALA-LIG-RXN, UDP-NACMURALGLDAPAALIG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 1 (33%)DLACTDEHYDROGNAD-RXN Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN0-6254 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 11 (100%)RXN-11482, RXN-11483, RXN-11479, RXN-11475, RXN-11474, RXN-11481, RXN-11477, RXN-11476, RXN-11478, RXN-11484, RXN-11480 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, GABATRANSAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)SUCCSEMIALDDEHYDROG-RXN, GABATRANSAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Unpredicted: 1OXALODECARB-RXN Entire module: 1 (8%)OXALODECARB-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1XMPXAN-RXN Entire module: 11 (100%)RXN-7609, IMP-DEHYDROG-RXN, AMP-DEPHOSPHORYLATION-RXN, RXN-7682, INOPHOSPHOR-RXN, XMPXAN-RXN, ADENODEAMIN-RXN, RXN0-5199, GUANINE-DEAMINASE-RXN, XANTHOSINEPHOSPHORY-RXN, RXN0-901 Blocked rxns: 1XMPXAN-RXN Essential rxns: 0	Annotated: 6 (75%)UDP-NACMURALA-GLU-LIG-RXN, DALADALALIG-RXN, GLUTRACE-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN, UDP-NACMUR-ALA-LIG-RXN Annot. missing: 2 (25%)UDP-NACMURALGLDAPLIG-RXN, UDP-NACMURALGLDAPAALIG-RXN Unpredicted: 0 Entire module: 6 (75%)UDP-NACMURALA-GLU-LIG-RXN, DALADALALIG-RXN, GLUTRACE-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN, UDP-NACMUR-ALA-LIG-RXN Blocked rxns: 0 Essential rxns: 6UDP-NACMURALA-GLU-LIG-RXN, DALADALALIG-RXN, GLUTRACE-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN, UDP-NACMUR-ALA-LIG-RXN	Unpredicted: 13.4.13.22-RXN Entire module: 2 (67%)3.4.13.22-RXN, DLACTDEHYDROGNAD-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 13.4.13.22-RXN Entire module: 1 (33%)3.4.13.22-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN0-6254 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1N-ACETYLGLUCOSAMINE-KINASE-RXN Entire module: 2 (67%)NAG6PDEACET-RXN, N-ACETYLGLUCOSAMINE-KINASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 11 (100%)RXN-11482, RXN-11483, RXN-11479, RXN-11475, RXN-11474, RXN-11481, RXN-11477, RXN-11476, RXN-11478, RXN-11484, RXN-11480 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, GABATRANSAM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)SUCCINATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, GABATRANSAM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)SUCCSEMIALDDEHYDROG-RXN, GABATRANSAM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)SUCCSEMIALDDEHYDROG-RXN, GABATRANSAM-RXN Blocked rxns: 0 Essential rxns: 0

	4-aminobenzoate biosynthesis	pyrimidine ribonucleosides salvage II	8-amino-7-oxononanoate biosynthesis III	superpathway of guanine and guanosine salvage	adenosine nucleotides degradation I	GDP-L-fucose biosynthesis I (from GDP-D-mannose)	superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation	adenine and adenosine salvage II	guanosine nucleotides degradation III	adenine and adenosine salvage III
e_coli_core	Annotated: 0 (0%) Annot. missing: 2 (100%)PABASYN-RXN, ADCLY-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)URIDINE-NUCLEOSIDASE-RXN, CYTIDEAM2-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)GUANPRIBOSYLTRAN-RXN, RXN0-5199, GUANOSINEKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)FCLEPIM-RXN, FCLREDUCT-RXN, GDPMANDEHYDRA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 4 (24%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN, ACETATEKIN-RXN, PHOSACETYLTRANS-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)ADENPRIBOSYLTRAN-RXN, ADENOSINE-NUCLEOSIDASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN-7609, GUANINE-DEAMINASE-RXN, RXN0-5199, RXN0-901 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)ADENODEAMIN-RXN, ADENPHOSPHOR-RXN, HYPOXANPRIBOSYLTRAN-RXN, INOPHOSPHOR-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 2 (100%)PABASYN-RXN, ADCLY-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)PABASYN-RXN, ADCLY-RXN Blocked rxns: 0 Essential rxns: 2PABASYN-RXN, ADCLY-RXN	Annotated: 2 (100%)URIDINE-NUCLEOSIDASE-RXN, CYTIDEAM2-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)URIDINE-NUCLEOSIDASE-RXN, CYTIDEAM2-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 17KAPSYN-RXN Entire module: 1 (50%)7KAPSYN-RXN Blocked rxns: 17KAPSYN-RXN Essential rxns: 0	Annotated: 3 (100%)GUANPRIBOSYLTRAN-RXN, RXN0-5199, GUANOSINEKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)GUANPRIBOSYLTRAN-RXN, RXN0-5199, GUANOSINEKIN-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 4RXN0-363, INOSINE-NUCLEOSIDASE-RXN, RXN-7607, XMPXAN-RXN Entire module: 7 (88%)IMP-DEHYDROG-RXN, INOSINE-NUCLEOSIDASE-RXN, RXN-7682, RXN0-363, XMPXAN-RXN, RXN-7607, RXN0-901 Blocked rxns: 2XMPXAN-RXN, RXN-7607 Essential rxns: 0	Annotated: 1 (33%)GDPMANDEHYDRA-RXN Annot. missing: 2 (67%)FCLEPIM-RXN, FCLREDUCT-RXN Unpredicted: 0 Entire module: 1 (33%)GDPMANDEHYDRA-RXN Blocked rxns: 1GDPMANDEHYDRA-RXN Essential rxns: 0	Unpredicted: 2RXN-11662, RXN-11667 Entire module: 8 (47%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN, RXN-11662, RXN-11667, ACETOACETYL-COA-TRANSFER-RXN, ACETYL-COA-ACETYLTRANSFER-RXN, PHOSACETYLTRANS-RXN, ACETATEKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)ADENPRIBOSYLTRAN-RXN, ADENOSINE-NUCLEOSIDASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)ADENPRIBOSYLTRAN-RXN, ADENOSINE-NUCLEOSIDASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (100%)RXN-7609, GUANINE-DEAMINASE-RXN, RXN0-5199, RXN0-901 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)RXN-7609, GUANINE-DEAMINASE-RXN, RXN0-5199, RXN0-901 Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (100%)ADENODEAMIN-RXN, ADENPHOSPHOR-RXN, HYPOXANPRIBOSYLTRAN-RXN, INOPHOSPHOR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)ADENODEAMIN-RXN, ADENPHOSPHOR-RXN, HYPOXANPRIBOSYLTRAN-RXN, INOPHOSPHOR-RXN Blocked rxns: 0 Essential rxns: 0

	adenine salvage	adenine and adenosine salvage V	superpathway of tetrahydrofolate biosynthesis	tetrahydrofolate biosynthesis	adenosine nucleotides degradation III	guanine and guanosine salvage III	adenine and adenosine salvage VI	guanine and guanosine salvage	cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate	queuosine biosynthesis
e_coli_core	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)ADENODEAMIN-RXN, INOSINEKIN-RXN, ADENPHOSPHOR-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 10 (100%)DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, H2PTEROATESYNTH-RXN, ADCLY-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, PABASYN-RXN, H2NEOPTERINALDOL-RXN, H2PTERIDINEPYROPHOSPHOKIN-RXN, DIHYDROFOLATESYNTH-RXN, DIHYDROFOLATEREDUCT-RXN, GTP-CYCLOHYDRO-I-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)DIHYDROFOLATEREDUCT-RXN, H2PTEROATESYNTH-RXN, DIHYDROFOLATESYNTH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)AMP-NUCLEOSID-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)GUANOSINEKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)GUANPRIBOSYLTRAN-RXN, RXN0-5199 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)RXN-12072, RXN-10040, RXN-12073, RXN-12070, RXN-12071 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN-12104, RXN0-1321, RXN0-4022, RXN0-1342 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Unpredicted: 1ADENINE-DEAMINASE-RXN Entire module: 3 (100%)ADENPRIBOSYLTRAN-RXN, HYPOXANPRIBOSYLTRAN-RXN, ADENINE-DEAMINASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)ADENODEAMIN-RXN, INOSINEKIN-RXN, ADENPHOSPHOR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)ADENODEAMIN-RXN, INOSINEKIN-RXN, ADENPHOSPHOR-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 9 (90%)DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, H2PTEROATESYNTH-RXN, ADCLY-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, PABASYN-RXN, H2NEOPTERINALDOL-RXN, H2PTERIDINEPYROPHOSPHOKIN-RXN, DIHYDROFOLATESYNTH-RXN, GTP-CYCLOHYDRO-I-RXN Annot. missing: 1 (10%)DIHYDROFOLATEREDUCT-RXN Unpredicted: 0 Entire module: 9 (90%)DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, H2PTEROATESYNTH-RXN, ADCLY-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, PABASYN-RXN, H2NEOPTERINALDOL-RXN, H2PTERIDINEPYROPHOSPHOKIN-RXN, DIHYDROFOLATESYNTH-RXN, GTP-CYCLOHYDRO-I-RXN Blocked rxns: 0 Essential rxns: 9DIHYDRONEOPTERIN-MONO-P-DEPHOS-RXN, H2PTEROATESYNTH-RXN, ADCLY-RXN, H2NEOPTERINP3PYROPHOSPHOHYDRO-RXN, PABASYN-RXN, H2NEOPTERINALDOL-RXN, H2PTERIDINEPYROPHOSPHOKIN-RXN, DIHYDROFOLATESYNTH-RXN, GTP-CYCLOHYDRO-I-RXN	Annotated: 2 (67%)H2PTEROATESYNTH-RXN, DIHYDROFOLATESYNTH-RXN Annot. missing: 1 (33%)DIHYDROFOLATEREDUCT-RXN Unpredicted: 0 Entire module: 2 (67%)H2PTEROATESYNTH-RXN, DIHYDROFOLATESYNTH-RXN Blocked rxns: 0 Essential rxns: 2H2PTEROATESYNTH-RXN, DIHYDROFOLATESYNTH-RXN	Annotated: 0 (0%) Annot. missing: 1 (100%)AMP-NUCLEOSID-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)GUANOSINEKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GUANOSINEKIN-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1ADENOSINE-KINASE-RXN Entire module: 1 (100%)ADENOSINE-KINASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)GUANPRIBOSYLTRAN-RXN, RXN0-5199 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)GUANPRIBOSYLTRAN-RXN, RXN0-5199 Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (40%)RXN-12073, RXN-10040 Annot. missing: 3 (60%)RXN-12072, RXN-12070, RXN-12071 Unpredicted: 0 Entire module: 2 (40%)RXN-12073, RXN-10040 Blocked rxns: 2RXN-12073, RXN-10040 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN-12104, RXN0-1321, RXN0-4022, RXN0-1342 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	preQ₀ biosynthesis	L-ascorbate degradation IV	ubiquinol-8 biosynthesis (prokaryotic)	sporopollenin precursors biosynthesis	S-methyl-5'-thioadenosine degradation I	molybdenum cofactor biosynthesis	pyruvate fermentation to hexanol (engineered)	4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis	thiazole biosynthesis I (facultative anaerobic bacteria)	thiamine diphosphate biosynthesis I (E. coli)
e_coli_core	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN-12093, RXN0-6575, GTP-CYCLOHYDRO-I-RXN, RXN0-5507 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-RXN, 2-OCTAPRENYLPHENOL-HYDROX-RXN, 2-OCTAPRENYL-6-METHOXYPHENOL-HYDROX-RXN, 2-OCTAPRENYL-6-OHPHENOL-METHY-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, DHHB-METHYLTRANSFER-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)RXN-8340, RXN-17809, RXN-8344, RXN-8342, RXN-8348, RXN-12473, RXN0-308, RXN-11361 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PYRIMSYN3-RXN, PYRIMSYN1-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)RXN-9788, DXS-RXN, THIAZOLSYN2-RXN, RXN-14382, RXN0-308, RXN-9789, RXN-11319 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN-12611, THI-P-KIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 1 (25%)GTP-CYCLOHYDRO-I-RXN Annot. missing: 3 (75%)RXN-12093, RXN0-6575, RXN0-5507 Unpredicted: 0 Entire module: 1 (25%)GTP-CYCLOHYDRO-I-RXN Blocked rxns: 0 Essential rxns: 1GTP-CYCLOHYDRO-I-RXN	Unpredicted: 1RXN-12107 Entire module: 1 (20%)RXN-12107 Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (62%)4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, 2-OCTAPRENYL-6-OHPHENOL-METHY-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, DHHB-METHYLTRANSFER-RXN Annot. missing: 3 (38%)2-OCTAPRENYLPHENOL-HYDROX-RXN, 2-OCTAPRENYL-6-METHOXYPHENOL-HYDROX-RXN, OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-RXN Unpredicted: 0 Entire module: 5 (62%)4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, 2-OCTAPRENYL-6-OHPHENOL-METHY-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, DHHB-METHYLTRANSFER-RXN Blocked rxns: 32-OCTAPRENYL-6-OHPHENOL-METHY-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, DHHB-METHYLTRANSFER-RXN Essential rxns: 23-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, 4OHBENZOATE-OCTAPRENYLTRANSFER-RXN	Unpredicted: 2PALMITOYL-COA-HYDROLASE-RXN, RXN-9624 Entire module: 2 (11%)PALMITOYL-COA-HYDROLASE-RXN, RXN-9624 Blocked rxns: 0 Essential rxns: 0	Unpredicted: 15-METHYLTHIORIBOSE-KINASE-RXN Entire module: 2 (100%)METHYLTHIOADENOSINE-NUCLEOSIDASE-RXN, 5-METHYLTHIORIBOSE-KINASE-RXN Blocked rxns: 2METHYLTHIOADENOSINE-NUCLEOSIDASE-RXN, 5-METHYLTHIORIBOSE-KINASE-RXN Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)RXN-8340, RXN-17809, RXN-8344, RXN-8342, RXN-8348, RXN-12473, RXN0-308, RXN-11361 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 5RXN-12570, RXN-11662, RXN-11667, RXN-12565, RXN-12567 Entire module: 6 (54%)RXN-12570, RXN-11662, RXN-11667, ACETYL-COA-ACETYLTRANSFER-RXN, RXN-12565, RXN-12567 Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PYRIMSYN3-RXN, PYRIMSYN1-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (14%)DXS-RXN Annot. missing: 6 (86%)RXN-9788, THIAZOLSYN2-RXN, RXN-14382, RXN0-308, RXN-9789, RXN-11319 Unpredicted: 0 Entire module: 1 (14%)DXS-RXN Blocked rxns: 0 Essential rxns: 1DXS-RXN	Annotated: 1 (50%)THI-P-KIN-RXN Annot. missing: 1 (50%)RXN-12611 Unpredicted: 0 Entire module: 1 (50%)THI-P-KIN-RXN Blocked rxns: 0 Essential rxns: 1THI-P-KIN-RXN

	thiamine salvage II	chitin derivatives degradation	hydroxymethylpyrimidine salvage	glycerophosphodiester degradation	L-ascorbate degradation II (bacterial, aerobic)	1,5-anhydrofructose degradation	glycerol degradation to butanol	fatty acid salvage	C4 photosynthetic carbon assimilation cycle, PEPCK type	UTP and CTP de novo biosynthesis
e_coli_core	Annotated: 0 (0%) Annot. missing: 5 (100%)PYRIMSYN3-RXN, THIAZOLSYN3-RXN, THI-P-SYN-RXN, THI-P-KIN-RXN, OHMETPYRKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PYRIMSYN3-RXN, OHMETPYRKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)GLYCPDIESTER-RXN, RXN0-5260, RXN-15740 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)RIBULPEPIM-RXN, LXULRU5P-RXN, RXN-12440, RXN0-704, RXN-12861, RXN-12862, RXN0-703, RXN0-705 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 1 (20%)PGLUCISOM-RXN Blocked rxns: 0 Essential rxns: 0	Entire module: 6 (38%)PHOSGLYPHOS-RXN, TRIOSEPISOMERIZATION-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 42PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PHOSGLYPHOS-RXN, 3PGAREARR-RXN	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 2 (17%)PEPCARBOXYKIN-RXN, MALIC-NADP-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)CTPSYN-RXN, RXN-12002, UDPKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 3 (60%)THI-P-KIN-RXN, THIAZOLSYN3-RXN, THI-P-SYN-RXN Annot. missing: 2 (40%)PYRIMSYN3-RXN, OHMETPYRKIN-RXN Unpredicted: 0 Entire module: 3 (60%)THI-P-KIN-RXN, THIAZOLSYN3-RXN, THI-P-SYN-RXN Blocked rxns: 1THIAZOLSYN3-RXN Essential rxns: 2THI-P-KIN-RXN, THI-P-SYN-RXN	Unpredicted: 1N-ACETYLGLUCOSAMINE-KINASE-RXN Entire module: 2 (25%)NAG6PDEACET-RXN, N-ACETYLGLUCOSAMINE-KINASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PYRIMSYN3-RXN, OHMETPYRKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)GLYCPDIESTER-RXN, RXN0-5260, RXN-15740 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (62%)RIBULPEPIM-RXN, LXULRU5P-RXN, RXN0-704, RXN0-703, RXN0-705 Annot. missing: 3 (38%)RXN-12862, RXN-12440, RXN-12861 Unpredicted: 0 Entire module: 5 (62%)RIBULPEPIM-RXN, LXULRU5P-RXN, RXN0-704, RXN0-703, RXN0-705 Blocked rxns: 3RXN0-703, RXN0-705, RXN0-704 Essential rxns: 0	Unpredicted: 1MANNKIN-RXN Entire module: 3 (60%)MANNKIN-RXN, PGLUCISOM-RXN, MANNPISOM-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2RXN-11662, RXN-11667 Entire module: 10 (62%)RXN-11662, RXN-11667, PHOSGLYPHOS-RXN, ACETYL-COA-ACETYLTRANSFER-RXN, GLYCDEH-RXN, TRIOSEPISOMERIZATION-RXN, 2PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 3RXN-13617, RXN-12490, RXN-13616 Entire module: 3 (50%)RXN-13617, RXN-12490, RXN-13616 Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2RXN-13697, RXN-13698 Entire module: 6 (50%)RXN-13697, PEPCARBOXYKIN-RXN, ALANINE-AMINOTRANSFERASE-RXN, RXN-13698, MALIC-NADP-RXN, ASPAMINOTRANS-RXN Blocked rxns: 0 Essential rxns: 4RXN-13697, ALANINE-AMINOTRANSFERASE-RXN, ASPAMINOTRANS-RXN, RXN-13698	Annotated: 3 (100%)CTPSYN-RXN, RXN-12002, UDPKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)CTPSYN-RXN, RXN-12002, UDPKIN-RXN Blocked rxns: 0 Essential rxns: 3CTPSYN-RXN, RXN-12002, UDPKIN-RXN

	purine deoxyribonucleosides degradation I	2'-deoxy-α-D-ribose 1-phosphate degradation	pyrimidine deoxyribonucleosides degradation	pyrimidine nucleobases salvage I	pyrimidine deoxyribonucleotides de novo biosynthesis I	UTP and CTP dephosphorylation I	pyrimidine deoxyribonucleotides de novo biosynthesis II	pyrimidine ribonucleosides salvage I	pyrimidine nucleobases salvage II	pyrimidine ribonucleosides salvage III
e_coli_core	Annotated: 0 (0%) Annot. missing: 4 (100%)ADDALT-RXN, DEOXYADENPHOSPHOR-RXN, DEOXYINOPHOSPHOR-RXN, DEOXYGUANPHOSPHOR-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (33%)ACETALD-DEHYDROG-RXN Annot. missing: 2 (67%)D-PPENTOMUT-RXN, DEOXYRIBOSE-P-ALD-RXN Unpredicted: 0 Entire module: 1 (33%)ACETALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)CYTIDEAM-RXN, THYM-PHOSPH-RXN, URA-PHOSPH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)URACIL-PRIBOSYLTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 9 (100%)UDPREDUCT-RXN, DUDPKIN-RXN, DTMPKI-RXN, DUTP-PYROP-RXN, THYMIDYLATESYN-RXN, RXN-12195, CDPREDUCT-RXN, DCDPKIN-RXN, DTDPKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)RXN0-724, DTMPKI-RXN, DUTP-PYROP-RXN, THYMIDYLATESYN-RXN, RXN0-723, DTDPKIN-RXN, DCTP-DEAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)CYTIDEAM2-RXN, URIDINEKIN-RXN, CYTIKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)URACIL-PRIBOSYLTRANS-RXN, CYTDEAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-361, CYTDEAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 4 (100%)ADDALT-RXN, DEOXYADENPHOSPHOR-RXN, DEOXYINOPHOSPHOR-RXN, DEOXYGUANPHOSPHOR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)ADDALT-RXN, DEOXYADENPHOSPHOR-RXN, DEOXYINOPHOSPHOR-RXN, DEOXYGUANPHOSPHOR-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)D-PPENTOMUT-RXN, ACETALD-DEHYDROG-RXN, DEOXYRIBOSE-P-ALD-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)D-PPENTOMUT-RXN, ACETALD-DEHYDROG-RXN, DEOXYRIBOSE-P-ALD-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)CYTIDEAM-RXN, THYM-PHOSPH-RXN, URA-PHOSPH-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)CYTIDEAM-RXN, THYM-PHOSPH-RXN, URA-PHOSPH-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)URACIL-PRIBOSYLTRANS-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)URACIL-PRIBOSYLTRANS-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 8 (89%)UDPREDUCT-RXN, DUDPKIN-RXN, DTMPKI-RXN, DUTP-PYROP-RXN, RXN-12195, CDPREDUCT-RXN, DCDPKIN-RXN, DTDPKIN-RXN Annot. missing: 1 (11%)THYMIDYLATESYN-RXN Unpredicted: 0 Entire module: 8 (89%)UDPREDUCT-RXN, DUDPKIN-RXN, DTMPKI-RXN, DUTP-PYROP-RXN, RXN-12195, DCDPKIN-RXN, CDPREDUCT-RXN, DTDPKIN-RXN Blocked rxns: 0 Essential rxns: 2DTMPKI-RXN, DTDPKIN-RXN	Unpredicted: 2RXN-14026, RXN-14025 Entire module: 3 (60%)CTPSYN-RXN, RXN-14026, RXN-14025 Blocked rxns: 0 Essential rxns: 1CTPSYN-RXN	Annotated: 4 (57%)DCTP-DEAM-RXN, DTMPKI-RXN, DUTP-PYROP-RXN, DTDPKIN-RXN Annot. missing: 3 (43%)RXN0-724, RXN0-723, THYMIDYLATESYN-RXN Unpredicted: 0 Entire module: 4 (57%)DCTP-DEAM-RXN, DTMPKI-RXN, DUTP-PYROP-RXN, DTDPKIN-RXN Blocked rxns: 0 Essential rxns: 2DTMPKI-RXN, DTDPKIN-RXN	Annotated: 1 (33%)CYTIDEAM2-RXN Annot. missing: 2 (67%)URIDINEKIN-RXN, CYTIKIN-RXN Unpredicted: 0 Entire module: 1 (33%)CYTIDEAM2-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)URACIL-PRIBOSYLTRANS-RXN, CYTDEAM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)URACIL-PRIBOSYLTRANS-RXN, CYTDEAM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)RXN0-361, CYTDEAM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)RXN0-361, CYTDEAM-RXN Blocked rxns: 0 Essential rxns: 0

	superpathway of pyrimidine ribonucleosides salvage	pyrimidine deoxyribonucleotide phosphorylation	pyrimidine deoxyribonucleosides salvage	superpathway of pyrimidine deoxyribonucleoside salvage	pyridoxal 5'-phosphate salvage II (plants)	CMP phosphorylation	pyrimidine deoxyribonucleotides dephosphorylation	superpathway of pyrimidine nucleobases salvage	superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered)
e_coli_core	Annotated: 0 (0%) Annot. missing: 10 (100%)CYTIKIN-RXN, URIDINE-NUCLEOSIDASE-RXN, RXN-11832, RXN-12002, URACIL-PRIBOSYLTRANS-RXN, CDPKIN-RXN, URIDINEKIN-RXN, UDPKIN-RXN, CTPSYN-RXN, CYTIDEAM2-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)DTMPKI-RXN, DCDPKIN-RXN, RXN-7913, DTDPKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)CDPKIN-RXN, RXN-11832 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)DCTP-PYROPHOSPHATASE-RXN, DUTP-PYROP-RXN, RXN-14188 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)CTPSYN-RXN, RXN-12002, URACIL-PRIBOSYLTRANS-RXN, UDPKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 18 (100%)CARBPSYN-RXN, DIHYDROOROT-RXN, DIHYDROOROTATE-DEHYDROGENASE-RXN, ASPCARBTRANS-RXN, UDPREDUCT-RXN, OROPRIBTRANS-RXN, DUDPKIN-RXN, RXN-12002, DTMPKI-RXN, THYMIDYLATESYN-RXN, DUTP-PYROP-RXN, RXN-12195, CDPREDUCT-RXN, DCDPKIN-RXN, DTDPKIN-RXN, CTPSYN-RXN, UDPKIN-RXN, OROTPDECARB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 8 (80%)URIDINE-NUCLEOSIDASE-RXN, RXN-11832, RXN-12002, URACIL-PRIBOSYLTRANS-RXN, CDPKIN-RXN, UDPKIN-RXN, CTPSYN-RXN, CYTIDEAM2-RXN Annot. missing: 2 (20%)URIDINEKIN-RXN, CYTIKIN-RXN Unpredicted: 0 Entire module: 8 (80%)URIDINE-NUCLEOSIDASE-RXN, RXN-11832, RXN-12002, URACIL-PRIBOSYLTRANS-RXN, CDPKIN-RXN, UDPKIN-RXN, CTPSYN-RXN, CYTIDEAM2-RXN Blocked rxns: 0 Essential rxns: 3CTPSYN-RXN, RXN-12002, UDPKIN-RXN	Annotated: 4 (100%)DTMPKI-RXN, DCDPKIN-RXN, RXN-7913, DTDPKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)DTMPKI-RXN, DCDPKIN-RXN, RXN-7913, DTDPKIN-RXN Blocked rxns: 0 Essential rxns: 2DTMPKI-RXN, DTDPKIN-RXN	Unpredicted: 2THYKI-RXN, DURIDKI-RXN Entire module: 3 (60%)CYTIDEAM-RXN, THYKI-RXN, DURIDKI-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2THYKI-RXN, DURIDKI-RXN Entire module: 7 (78%)CYTIDEAM-RXN, DURIDKI-RXN, DTMPKI-RXN, THYKI-RXN, DCDPKIN-RXN, DTDPKIN-RXN, RXN-7913 Blocked rxns: 0 Essential rxns: 2DTMPKI-RXN, DTDPKIN-RXN	Unpredicted: 3RXN-14046, RXN-14181, 3.1.3.74-RXN Entire module: 8 (89%)RXN-14181, PYRIDOXKIN-RXN, PYRAMKIN-RXN, RXN-14046, 3.1.3.74-RXN, PNKIN-RXN, PNPOXI-RXN, PMPOXI-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)CDPKIN-RXN, RXN-11832 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)CDPKIN-RXN, RXN-11832 Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (67%)DCTP-PYROPHOSPHATASE-RXN, DUTP-PYROP-RXN Annot. missing: 1 (33%)RXN-14188 Unpredicted: 0 Entire module: 2 (67%)DCTP-PYROPHOSPHATASE-RXN, DUTP-PYROP-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (100%)CTPSYN-RXN, RXN-12002, URACIL-PRIBOSYLTRANS-RXN, UDPKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)CTPSYN-RXN, RXN-12002, URACIL-PRIBOSYLTRANS-RXN, UDPKIN-RXN Blocked rxns: 0 Essential rxns: 3CTPSYN-RXN, RXN-12002, UDPKIN-RXN	Annotated: 16 (89%)CARBPSYN-RXN, DIHYDROOROT-RXN, ASPCARBTRANS-RXN, UDPREDUCT-RXN, OROPRIBTRANS-RXN, DUDPKIN-RXN, RXN-12002, DTMPKI-RXN, DUTP-PYROP-RXN, RXN-12195, CDPREDUCT-RXN, DCDPKIN-RXN, DTDPKIN-RXN, CTPSYN-RXN, UDPKIN-RXN, OROTPDECARB-RXN Annot. missing: 2 (11%)DIHYDROOROTATE-DEHYDROGENASE-RXN, THYMIDYLATESYN-RXN Unpredicted: 0 Entire module: 16 (89%)CARBPSYN-RXN, DIHYDROOROT-RXN, ASPCARBTRANS-RXN, UDPREDUCT-RXN, OROPRIBTRANS-RXN, DUDPKIN-RXN, RXN-12002, DTMPKI-RXN, DUTP-PYROP-RXN, RXN-12195, CDPREDUCT-RXN, DCDPKIN-RXN, DTDPKIN-RXN, CTPSYN-RXN, UDPKIN-RXN, OROTPDECARB-RXN Blocked rxns: 0 Essential rxns: 9ASPCARBTRANS-RXN, DIHYDROOROT-RXN, OROPRIBTRANS-RXN, RXN-12002, DTMPKI-RXN, UDPKIN-RXN, DTDPKIN-RXN, CTPSYN-RXN, OROTPDECARB-RXN	Unpredicted: 1RXN-11662 Entire module: 2 (40%)ACETYL-COA-ACETYLTRANSFER-RXN, RXN-11662 Blocked rxns: 0 Essential rxns: 0

	photosynthetic 3-hydroxybutanoate biosynthesis (engineered)	adenosine ribonucleotides de novo biosynthesis	adenosine deoxyribonucleotides de novo biosynthesis II	guanosine ribonucleotides de novo biosynthesis	guanosine deoxyribonucleotides de novo biosynthesis II	purine deoxyribonucleosides salvage	D-fructuronate degradation	superpathway NAD/NADP - NADH/NADPH interconversion (yeast)	β-D-glucuronide and D-glucuronate degradation	NAD/NADP-NADH/NADPH cytosolic interconversion (yeast)
e_coli_core	Entire module: 10 (38%)F16BDEPHOS-RXN, PHOSGLYPHOS-RXN, 2TRANSKETO-RXN, TRIOSEPISOMERIZATION-RXN, PYRUVDEH-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, RIBULP3EPIM-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 32PGADEHYDRAT-RXN, PHOSGLYPHOS-RXN, 3PGAREARR-RXN	Annotated: 1 (33%)ADENYL-KIN-RXN Annot. missing: 2 (67%)ADENYLOSUCCINATE-SYNTHASE-RXN, AMPSYN-RXN Unpredicted: 0 Entire module: 1 (33%)ADENYL-KIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)DADPKIN-RXN, ADPREDUCT-RXN, RXN0-745, RXN0-747 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)GDPKIN-RXN, GMP-SYN-GLUT-RXN, GUANYL-KIN-RXN, IMP-DEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN0-746, DGDPKIN-RXN, GDPREDUCT-RXN, RXN0-748 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)MANNONDEHYDRAT-RXN, MANNONOXIDOREDUCT-RXN, DEOXYGLUCONOKIN-RXN, KDPGALDOL-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 1 (12%)GLU6PDEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)GLUCUROISOM-RXN, BETA-GLUCURONID-RXN, RXN-14693 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 1 (20%)GLU6PDEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0
iAF1260	Unpredicted: 1RXN-11662 Entire module: 12 (46%)RXN-11662, F16BDEPHOS-RXN, PHOSGLYPHOS-RXN, 2TRANSKETO-RXN, ACETYL-COA-ACETYLTRANSFER-RXN, TRIOSEPISOMERIZATION-RXN, PYRUVDEH-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, RIBULP3EPIM-RXN, PEPDEPHOS-RXN, 3PGAREARR-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)ADENYLOSUCCINATE-SYNTHASE-RXN, ADENYL-KIN-RXN, AMPSYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)ADENYLOSUCCINATE-SYNTHASE-RXN, ADENYL-KIN-RXN, AMPSYN-RXN Blocked rxns: 0 Essential rxns: 2ADENYLOSUCCINATE-SYNTHASE-RXN, AMPSYN-RXN	Annotated: 2 (50%)DADPKIN-RXN, ADPREDUCT-RXN Annot. missing: 2 (50%)RXN0-745, RXN0-747 Unpredicted: 0 Entire module: 2 (50%)DADPKIN-RXN, ADPREDUCT-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (100%)GDPKIN-RXN, GMP-SYN-GLUT-RXN, GUANYL-KIN-RXN, IMP-DEHYDROG-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)GDPKIN-RXN, GMP-SYN-GLUT-RXN, GUANYL-KIN-RXN, IMP-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 2GMP-SYN-GLUT-RXN, GUANYL-KIN-RXN	Annotated: 2 (50%)DGDPKIN-RXN, GDPREDUCT-RXN Annot. missing: 2 (50%)RXN0-746, RXN0-748 Unpredicted: 0 Entire module: 2 (50%)DGDPKIN-RXN, GDPREDUCT-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2DEOXYADENYLATE-KINASE-RXN, GMKALT-RXN Entire module: 4 (67%)DADPKIN-RXN, DEOXYADENYLATE-KINASE-RXN, GMKALT-RXN, DGDPKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (100%)MANNONDEHYDRAT-RXN, MANNONOXIDOREDUCT-RXN, DEOXYGLUCONOKIN-RXN, KDPGALDOL-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)MANNONDEHYDRAT-RXN, MANNONOXIDOREDUCT-RXN, DEOXYGLUCONOKIN-RXN, KDPGALDOL-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1RXN0-3962 Entire module: 3 (38%)GLU6PDEHYDROG-RXN, NAD-KIN-RXN, RXN0-3962 Blocked rxns: 0 Essential rxns: 1NAD-KIN-RXN	Annotated: 0 (0%) Annot. missing: 3 (100%)GLUCUROISOM-RXN, BETA-GLUCURONID-RXN, RXN-14693 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1RXN0-3962 Entire module: 3 (60%)GLU6PDEHYDROG-RXN, NAD-KIN-RXN, RXN0-3962 Blocked rxns: 0 Essential rxns: 1NAD-KIN-RXN

	L-arabinose degradation IV	dTDP-N-acetylthomosamine biosynthesis	UDP-N-acetyl-α-D-mannosaminouronate biosynthesis	UDP-α-D-glucose biosynthesis I	UDP-α-D-galactose biosynthesis	UDP-α-D-glucuronate biosynthesis (from UDP-glucose)	phosphatidylcholine resynthesis via glycerophosphocholine	1,3-propanediol biosynthesis (engineered)	hypotaurine degradation	octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast)
e_coli_core	Entire module: 1 (12%)MALSYN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)TDPFUCACTRANS-RXN, DTDPGLUCOSEPP-RXN, RFFTRANS-RXN, DTDPGLUCDEHYDRAT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)UDPMANNACADEHYDROG-RXN, UDPGLCNACEPIM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)PHOSPHOGLUCMUT-RXN, GLUC1PURIDYLTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)UDPGLUCEPIM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)UGD-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 3 (33%)6PFRUCTPHOS-RXN, PGLUCISOM-RXN, F16ALDOLASE-RXN Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Unpredicted: 1GLYCOLATE-REDUCTASE-RXN Entire module: 2 (25%)MALSYN-RXN, GLYCOLATE-REDUCTASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (100%)TDPFUCACTRANS-RXN, DTDPGLUCOSEPP-RXN, RFFTRANS-RXN, DTDPGLUCDEHYDRAT-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)TDPFUCACTRANS-RXN, DTDPGLUCOSEPP-RXN, RFFTRANS-RXN, DTDPGLUCDEHYDRAT-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)UDPMANNACADEHYDROG-RXN, UDPGLCNACEPIM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)UDPMANNACADEHYDROG-RXN, UDPGLCNACEPIM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)PHOSPHOGLUCMUT-RXN, GLUC1PURIDYLTRANS-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)PHOSPHOGLUCMUT-RXN, GLUC1PURIDYLTRANS-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)UDPGLUCEPIM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)UDPGLUCEPIM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)UGD-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)UGD-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 13.1.4.2-RXN Entire module: 1 (33%)3.1.4.2-RXN Blocked rxns: 13.1.4.2-RXN Essential rxns: 0	Unpredicted: 1RXN-14965 Entire module: 5 (56%)GLUCOKIN-RXN, PGLUCISOM-RXN, RXN-14965, 6PFRUCTPHOS-RXN, F16ALDOLASE-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1RXN66-3 Entire module: 1 (33%)RXN66-3 Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1ACETYL-COA-CARBOXYLTRANSFER-RXN Entire module: 3 (43%)ACETYL-COA-CARBOXYLTRANSFER-RXN, MALONYL-COA-ACP-TRANSACYL-RXN, 3-OXOACYL-ACP-SYNTH-BASE-RXN Blocked rxns: 0 Essential rxns: 2MALONYL-COA-ACP-TRANSACYL-RXN, ACETYL-COA-CARBOXYLTRANSFER-RXN

	phospholipid remodeling (phosphatidylethanolamine, yeast)	aromatic biogenic amine degradation (bacteria)	sulfoquinovose degradation I	L-methionine salvage cycle I (bacteria and plants)	pyruvate to cytochrome bo oxidase electron transfer	pyruvate to cytochrome bd oxidase electron transfer	glutathione degradation (DUG pathway - yeast)	(8E,10E)-dodeca-8,10-dienol biosynthesis	Spodoptera littoralis pheromone biosynthesis	bacterial bioluminescence
e_coli_core	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN-15298, RXN-15299, RXN-15296, RXN-15297 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5268, RXN-11496 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5266, RXN-11496 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Unpredicted: 1RXN-14160 Entire module: 1 (25%)RXN-14160 Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2RXN6666-4, RXN-5821 Entire module: 2 (25%)RXN6666-4, RXN-5821 Blocked rxns: 1RXN6666-4 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN-15298, RXN-15299, RXN-15296, RXN-15297 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 3R145-RXN, 5.3.1.23-RXN, 5-METHYLTHIORIBOSE-KINASE-RXN Entire module: 7 (58%)S-ADENMETSYN-RXN, R145-RXN, 5-METHYLTHIORIBOSE-KINASE-RXN, SPERMIDINESYN-RXN, SAMDECARB-RXN, METHYLTHIOADENOSINE-NUCLEOSIDASE-RXN, 5.3.1.23-RXN Blocked rxns: 6R145-RXN, 5-METHYLTHIORIBOSE-KINASE-RXN, SPERMIDINESYN-RXN, SAMDECARB-RXN, METHYLTHIOADENOSINE-NUCLEOSIDASE-RXN, 5.3.1.23-RXN Essential rxns: 1S-ADENMETSYN-RXN	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5268, RXN-11496 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5266, RXN-11496 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2RXN-6622, RXN-12618 Entire module: 2 (100%)RXN-6622, RXN-12618 Blocked rxns: 0 Essential rxns: 0	Unpredicted: 6RXN-12507, RXN-14268, 2.3.1.155-RXN, RXN-14272, RXN-14273, RXN-14271 Entire module: 6 (54%)RXN-12507, RXN-14268, 2.3.1.155-RXN, RXN-14272, RXN-14273, RXN-14271 Blocked rxns: 0 Essential rxns: 0	Unpredicted: 3RXN-14271, RXN-14272, 2.3.1.155-RXN Entire module: 3 (14%)RXN-14271, RXN-14272, 2.3.1.155-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1RXN-9510 Entire module: 2 (25%)RXN-12444, RXN-9510 Blocked rxns: 0 Essential rxns: 0

	NAD salvage pathway II	aminomethylphosphonate degradation	tunicamycin biosynthesis	heme a biosynthesis	L-cysteine biosynthesis VII	tRNA-uridine 2-thiolation (bacteria)	adenosylcobinamide-GDP salvage from cobinamide I	ATP biosynthesis	homocysteine and cysteine interconversion	L-alanine biosynthesis III
e_coli_core	Annotated: 0 (0%) Annot. missing: 4 (100%)NICONUCADENYLYLTRAN-RXN, NMNAMIDOHYDRO-RXN, NADPYROPHOSPHAT-RXN, NAD-SYNTH-GLN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)RXN-17956, RXN-17954, RXN-17955, RXN0-7014, ADENPRIBOSYLTRAN-RXN, RXN0-1401, INORGPYROPHOSPHAT-RXN, RXN0-6710 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN-18586, SULFOCYS-RXN, PRODISULFREDUCT-A-RXN, SERINE-O-ACETTRAN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 8 (100%)RXN-18709, RXN-18708, RXN0-7081, RXN0-5144, RXN-18707, RXN-18710, RXN0-308, RXN0-2023 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)RXN-19343, COBINAMIDEKIN-RXN, RXN-19338, RXN-19342, COBINPGUANYLYLTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ATPSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN0-308 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 3 (75%)NICONUCADENYLYLTRAN-RXN, NMNAMIDOHYDRO-RXN, NADPYROPHOSPHAT-RXN Annot. missing: 1 (25%)NAD-SYNTH-GLN-RXN Unpredicted: 0 Entire module: 3 (75%)NICONUCADENYLYLTRAN-RXN, NMNAMIDOHYDRO-RXN, NADPYROPHOSPHAT-RXN Blocked rxns: 0 Essential rxns: 1NICONUCADENYLYLTRAN-RXN	Annotated: 3 (38%)ADENPRIBOSYLTRAN-RXN, RXN0-1401, INORGPYROPHOSPHAT-RXN Annot. missing: 5 (62%)RXN-17956, RXN-17954, RXN-17955, RXN0-7014, RXN0-6710 Unpredicted: 0 Entire module: 3 (38%)ADENPRIBOSYLTRAN-RXN, RXN0-1401, INORGPYROPHOSPHAT-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2RXN-14025, RXN-14139 Entire module: 2 (22%)RXN-14025, RXN-14139 Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1HEMEOSYN-RXN Entire module: 1 (25%)HEMEOSYN-RXN Blocked rxns: 1HEMEOSYN-RXN Essential rxns: 0	Annotated: 1 (25%)SERINE-O-ACETTRAN-RXN Annot. missing: 3 (75%)RXN-18586, SULFOCYS-RXN, PRODISULFREDUCT-A-RXN Unpredicted: 0 Entire module: 1 (25%)SERINE-O-ACETTRAN-RXN Blocked rxns: 0 Essential rxns: 1SERINE-O-ACETTRAN-RXN	Annotated: 0 (0%) Annot. missing: 8 (100%)RXN-18709, RXN-18708, RXN0-7081, RXN0-5144, RXN-18707, RXN-18710, RXN0-308, RXN0-2023 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (40%)COBINAMIDEKIN-RXN, COBINPGUANYLYLTRANS-RXN Annot. missing: 3 (60%)RXN-19342, RXN-19343, RXN-19338 Unpredicted: 0 Entire module: 2 (40%)COBINAMIDEKIN-RXN, COBINPGUANYLYLTRANS-RXN Blocked rxns: 2COBINAMIDEKIN-RXN, COBINPGUANYLYLTRANS-RXN Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ATPSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1CYSTATHIONINE-BETA-LYASE-RXN Entire module: 2 (50%)CYSTATHIONINE-BETA-LYASE-RXN, O-SUCCHOMOSERLYASE-RXN Blocked rxns: 0 Essential rxns: 2CYSTATHIONINE-BETA-LYASE-RXN, O-SUCCHOMOSERLYASE-RXN	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN0-308 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	superpathway of L-alanine biosynthesis	trehalose degradation II (trehalase)	putrescine degradation II	ADP-L-glycero-β-D-manno-heptose biosynthesis	anhydromuropeptides recycling I	biotin-carboxyl carrier protein assembly	lipoate biosynthesis and incorporation II	3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation	ethylene glycol degradation	pyrimidine ribonucleosides degradation
e_coli_core	Annotated: 0 (0%) Annot. missing: 5 (100%)ALANINE-AMINOTRANSFERASE-RXN, VALINE-PYRUVATE-AMINOTRANSFER-RXN, ALARACECAT-RXN, BRANCHED-CHAINAMINOTRANSFERVAL-RXN, RXN0-308 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)GLUCOKIN-RXN, TREHALA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN0-3901, RXN0-3922, RXN0-3942, RXN0-3921 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)RXN0-4301, 5.1.3.20-RXN, RXN0-4342, RXN0-4341, RXN0-4361 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 12 (100%)RXN0-2361, 2.3.1.157-RXN, RXN0-4621, RXN0-4641, RXN0-5226, 5.4.2.10-RXN, NAG6PDEACET-RXN, NAG1P-URIDYLTRANS-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN, RXN0-5227, RXN0-5225 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)BIOTINLIG-RXN, RXN0-5055, RXN-7101, BIOTIN-CARBOXYL-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-949, RXN0-5098 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (12%)ACETALD-DEHYDROG-RXN Annot. missing: 7 (88%)PHENPRODIOLDEHYDROG-RXN, MHPELY-RXN, HCAMULTI-RXN, MHPHYDROXY-RXN, MHPCHYDROL-RXN, 2-OXOPENT-4-ENOATE-HYDRATASE-RXN, 1.13.11.16-RXN Unpredicted: 0 Entire module: 1 (12%)ACETALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)GLYCOLALDREDUCT-RXN, GLYCOLALD-DEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)CYTIDEAM2-RXN, URPHOS-RXN, PPENTOMUT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 4 (80%)ALARACECAT-RXN, ALANINE-AMINOTRANSFERASE-RXN, BRANCHED-CHAINAMINOTRANSFERVAL-RXN, VALINE-PYRUVATE-AMINOTRANSFER-RXN Annot. missing: 1 (20%)RXN0-308 Unpredicted: 0 Entire module: 4 (80%)ALARACECAT-RXN, ALANINE-AMINOTRANSFERASE-RXN, BRANCHED-CHAINAMINOTRANSFERVAL-RXN, VALINE-PYRUVATE-AMINOTRANSFER-RXN Blocked rxns: 0 Essential rxns: 2ALARACECAT-RXN, ALANINE-AMINOTRANSFERASE-RXN	Annotated: 1 (50%)GLUCOKIN-RXN Annot. missing: 1 (50%)TREHALA-RXN Unpredicted: 0 Entire module: 1 (50%)GLUCOKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (25%)RXN0-3942 Annot. missing: 3 (75%)RXN0-3901, RXN0-3922, RXN0-3921 Unpredicted: 0 Entire module: 1 (25%)RXN0-3942 Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (100%)RXN0-4301, 5.1.3.20-RXN, RXN0-4342, RXN0-4341, RXN0-4361 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)RXN0-4301, 5.1.3.20-RXN, RXN0-4342, RXN0-4341, RXN0-4361 Blocked rxns: 0 Essential rxns: 0	Annotated: 7 (58%)2.3.1.157-RXN, 5.4.2.10-RXN, RXN0-4641, NAG6PDEACET-RXN, NAG1P-URIDYLTRANS-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN Annot. missing: 5 (42%)RXN0-2361, RXN0-4621, RXN0-5226, RXN0-5227, RXN0-5225 Unpredicted: 0 Entire module: 7 (58%)2.3.1.157-RXN, 5.4.2.10-RXN, RXN0-4641, NAG6PDEACET-RXN, NAG1P-URIDYLTRANS-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN Blocked rxns: 0 Essential rxns: 52.3.1.157-RXN, 5.4.2.10-RXN, NAG1P-URIDYLTRANS-RXN, UDPNACETYLGLUCOSAMENOLPYRTRANS-RXN, UDPNACETYLMURAMATEDEHYDROG-RXN	Annotated: 0 (0%) Annot. missing: 4 (100%)BIOTINLIG-RXN, RXN0-5055, RXN-7101, BIOTIN-CARBOXYL-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-949, RXN0-5098 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (75%)PHENPRODIOLDEHYDROG-RXN, ACETALD-DEHYDROG-RXN, MHPELY-RXN, HCAMULTI-RXN, MHPHYDROXY-RXN, 1.13.11.16-RXN Annot. missing: 2 (25%)2-OXOPENT-4-ENOATE-HYDRATASE-RXN, MHPCHYDROL-RXN Unpredicted: 0 Entire module: 6 (75%)PHENPRODIOLDEHYDROG-RXN, ACETALD-DEHYDROG-RXN, MHPELY-RXN, HCAMULTI-RXN, MHPHYDROXY-RXN, 1.13.11.16-RXN Blocked rxns: 5PHENPRODIOLDEHYDROG-RXN, MHPELY-RXN, HCAMULTI-RXN, MHPHYDROXY-RXN, 1.13.11.16-RXN Essential rxns: 0	Annotated: 1 (50%)GLYCOLALD-DEHYDROG-RXN Annot. missing: 1 (50%)GLYCOLALDREDUCT-RXN Unpredicted: 0 Entire module: 1 (50%)GLYCOLALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 1GLYCOLALD-DEHYDROG-RXN	Annotated: 2 (67%)CYTIDEAM2-RXN, URPHOS-RXN Annot. missing: 1 (33%)PPENTOMUT-RXN Unpredicted: 0 Entire module: 2 (100%)CYTIDEAM2-RXN, URPHOS-RXN Blocked rxns: 0 Essential rxns: 0

	purine ribonucleosides degradation	superpathway of purine deoxyribonucleosides degradation	superpathway of pyrimidine deoxyribonucleosides degradation	arginine dependent acid resistance	2-O-α-mannosyl-D-glycerate degradation	melibiose degradation	aminopropylcadaverine biosynthesis	L-glutamate degradation IX (via 4-aminobutanoate)	L-galactonate degradation	chitobiose degradation
e_coli_core	Annotated: 0 (0%) Annot. missing: 6 (100%)ADENPHOSPHOR-RXN, INOPHOSPHOR-RXN, ADENODEAMIN-RXN, RXN0-5199, PPENTOMUT-RXN, XANTHOSINEPHOSPHORY-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (14%)ACETALD-DEHYDROG-RXN Annot. missing: 6 (86%)DEOXYRIBOSE-P-ALD-RXN, ADDALT-RXN, D-PPENTOMUT-RXN, DEOXYINOPHOSPHOR-RXN, DEOXYADENPHOSPHOR-RXN, DEOXYGUANPHOSPHOR-RXN Unpredicted: 0 Entire module: 1 (14%)ACETALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (17%)ACETALD-DEHYDROG-RXN Annot. missing: 5 (83%)CYTIDEAM-RXN, THYM-PHOSPH-RXN, DEOXYRIBOSE-P-ALD-RXN, URA-PHOSPH-RXN, D-PPENTOMUT-RXN Unpredicted: 0 Entire module: 1 (17%)ACETALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ARGDECARBOX-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)GKI-RXN, RXN0-5216 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ALPHAGALACTOSID-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5217, LYSDECARBOX-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)GLUTDECARBOX-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN0-5229 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-7002, RXN0-7001 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 5 (83%)ADENPHOSPHOR-RXN, INOPHOSPHOR-RXN, ADENODEAMIN-RXN, RXN0-5199, XANTHOSINEPHOSPHORY-RXN Annot. missing: 1 (17%)PPENTOMUT-RXN Unpredicted: 0 Entire module: 5 (83%)ADENPHOSPHOR-RXN, INOPHOSPHOR-RXN, ADENODEAMIN-RXN, RXN0-5199, XANTHOSINEPHOSPHORY-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 7 (100%)ACETALD-DEHYDROG-RXN, DEOXYRIBOSE-P-ALD-RXN, ADDALT-RXN, D-PPENTOMUT-RXN, DEOXYINOPHOSPHOR-RXN, DEOXYADENPHOSPHOR-RXN, DEOXYGUANPHOSPHOR-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 7 (100%)ACETALD-DEHYDROG-RXN, DEOXYRIBOSE-P-ALD-RXN, ADDALT-RXN, D-PPENTOMUT-RXN, DEOXYINOPHOSPHOR-RXN, DEOXYADENPHOSPHOR-RXN, DEOXYGUANPHOSPHOR-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (100%)CYTIDEAM-RXN, ACETALD-DEHYDROG-RXN, THYM-PHOSPH-RXN, DEOXYRIBOSE-P-ALD-RXN, URA-PHOSPH-RXN, D-PPENTOMUT-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 6 (100%)CYTIDEAM-RXN, ACETALD-DEHYDROG-RXN, THYM-PHOSPH-RXN, DEOXYRIBOSE-P-ALD-RXN, URA-PHOSPH-RXN, D-PPENTOMUT-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)ARGDECARBOX-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ARGDECARBOX-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)GKI-RXN, RXN0-5216 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)GKI-RXN, RXN0-5216 Blocked rxns: 1RXN0-5216 Essential rxns: 0	Annotated: 1 (100%)ALPHAGALACTOSID-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ALPHAGALACTOSID-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (50%)LYSDECARBOX-RXN Annot. missing: 1 (50%)RXN0-5217 Unpredicted: 0 Entire module: 1 (50%)LYSDECARBOX-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)GLUTDECARBOX-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)GLUTDECARBOX-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)RXN0-5229 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)RXN0-5229 Blocked rxns: 1RXN0-5229 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-7002, RXN0-7001 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	acetate formation from acetyl-CoA I	acetate conversion to acetyl-CoA	fructose degradation	L-lactaldehyde degradation (anaerobic)	L-lactaldehyde degradation (aerobic)	CDP-diacylglycerol biosynthesis II	nitrate reduction III (dissimilatory)	N-acetylneuraminate and N-acetylmannosamine degradation I	superpathway of L-asparagine biosynthesis	succinate to cytochrome bo oxidase electron transfer
e_coli_core	Annotated: 2 (100%)PHOSACETYLTRANS-RXN, ACETATEKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)PHOSACETYLTRANS-RXN, ACETATEKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)ACETATE--COA-LIGASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)1PFRUCTPHOSN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)LACTALDREDUCT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)L-LACTDEHYDROGFMN-RXN, LACTALDDEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)CDPDIGLYSYN-RXN, RXN-10462, 1-ACYLGLYCEROL-3-P-ACYLTRANSFER-RXN, GLYC3PDEHYDROGBIOSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-3501, FORMATEDEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)ACNEULY-RXN, NANE-RXN, NANK-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)ASNSYNA-RXN, ASNSYNB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)SUCCINATE-DEHYDROGENASE-UBIQUINONE-RXN, RXN0-5268 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 2 (100%)PHOSACETYLTRANS-RXN, ACETATEKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)PHOSACETYLTRANS-RXN, ACETATEKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)ACETATE--COA-LIGASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)ACETATE--COA-LIGASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)1PFRUCTPHOSN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)LACTALDREDUCT-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)LACTALDREDUCT-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (50%)LACTALDDEHYDROG-RXN Annot. missing: 1 (50%)L-LACTDEHYDROGFMN-RXN Unpredicted: 0 Entire module: 1 (50%)LACTALDDEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)CDPDIGLYSYN-RXN, RXN-10462, 1-ACYLGLYCEROL-3-P-ACYLTRANSFER-RXN, GLYC3PDEHYDROGBIOSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-3501, FORMATEDEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)ACNEULY-RXN, NANE-RXN, NANK-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)ACNEULY-RXN, NANE-RXN, NANK-RXN Blocked rxns: 1ACNEULY-RXN Essential rxns: 0	Annotated: 2 (100%)ASNSYNA-RXN, ASNSYNB-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)ASNSYNA-RXN, ASNSYNB-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)SUCCINATE-DEHYDROGENASE-UBIQUINONE-RXN, RXN0-5268 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	NADH to cytochrome bd oxidase electron transfer I	NADH to cytochrome bo oxidase electron transfer I	NADH to fumarate electron transfer	oleate β-oxidation	polymyxin resistance	NADH to trimethylamine N-oxide electron transfer	NADH to dimethyl sulfoxide electron transfer	nitrate reduction VIII (dissimilatory)	succinate to cytochrome bd oxidase electron transfer	formate to trimethylamine N-oxide electron transfer
e_coli_core	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5266, NADH-DEHYDROG-A-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5268, NADH-DEHYDROG-A-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)R601-RXN, RXN0-5388 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-5390, RXN0-5393, RXN0-5391 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)RXN0-1863, RXN0-1862, RXN0-5409, RXN0-3521, RXN0-2001, RXN0-1861 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5388, RXN0-5264 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5388, DIMESULFREDUCT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-3501, RXN0-5388 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5266, SUCCINATE-DEHYDROGENASE-UBIQUINONE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)FORMATEDEHYDROG-RXN, RXN0-5264 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5266, NADH-DEHYDROG-A-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5268, NADH-DEHYDROG-A-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)R601-RXN, RXN0-5388 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-5390, RXN0-5393, RXN0-5391 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (33%)RXN0-1863, RXN0-1861 Annot. missing: 4 (67%)RXN0-2001, RXN0-1862, RXN0-5409, RXN0-3521 Unpredicted: 0 Entire module: 2 (33%)RXN0-1863, RXN0-1861 Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5388, RXN0-5264 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5388, DIMESULFREDUCT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-3501, RXN0-5388 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5266, SUCCINATE-DEHYDROGENASE-UBIQUINONE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)FORMATEDEHYDROG-RXN, RXN0-5264 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	formate to dimethyl sulfoxide electron transfer	S-methyl-5'-thioadenosine degradation IV	superpathway of heme b biosynthesis from uroporphyrinogen-III	tetrahydromonapterin biosynthesis	D-malate degradation	trehalose degradation VI (periplasmic)	uracil degradation III	ethanolamine utilization	biotin biosynthesis from 8-amino-7-oxononanoate I	sedoheptulose bisphosphate bypass
e_coli_core	Annotated: 0 (0%) Annot. missing: 2 (100%)FORMATEDEHYDROG-RXN, DIMESULFREDUCT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)METHYLTHIOADENOSINE-NUCLEOSIDASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)PROTOPORGENOXI-RXN, RXN0-1461, UROGENDECARBOX-RXN, HEMN-RXN, RXN0-6259, PROTOHEMEFERROCHELAT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN0-6368, H2NTPEPIM-RXN, RXN0-6367, GTP-CYCLOHYDRO-I-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)1.1.1.83-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)ALDOSE-1-EPIMERASE-RXN, TREHALA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)RXN0-6444, RXN0-6460, RXN-8974, RXN0-6452, RXN0-5222 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (80%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN, ACETATEKIN-RXN, PHOSACETYLTRANS-RXN Annot. missing: 1 (20%)ETHAMLY-RXN Unpredicted: 0 Entire module: 4 (80%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN, ACETATEKIN-RXN, PHOSACETYLTRANS-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)2.8.1.6-RXN, DAPASYN-RXN, DETHIOBIOTIN-SYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)SEDOBISALDOL-RXN, RXN0-6541 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 0 (0%) Annot. missing: 2 (100%)FORMATEDEHYDROG-RXN, DIMESULFREDUCT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)METHYLTHIOADENOSINE-NUCLEOSIDASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)METHYLTHIOADENOSINE-NUCLEOSIDASE-RXN Blocked rxns: 1METHYLTHIOADENOSINE-NUCLEOSIDASE-RXN Essential rxns: 0	Annotated: 4 (67%)RXN0-1461, UROGENDECARBOX-RXN, HEMN-RXN, PROTOHEMEFERROCHELAT-RXN Annot. missing: 2 (33%)RXN0-6259, PROTOPORGENOXI-RXN Unpredicted: 0 Entire module: 4 (67%)RXN0-1461, UROGENDECARBOX-RXN, HEMN-RXN, PROTOHEMEFERROCHELAT-RXN Blocked rxns: 0 Essential rxns: 2UROGENDECARBOX-RXN, PROTOHEMEFERROCHELAT-RXN	Annotated: 1 (25%)GTP-CYCLOHYDRO-I-RXN Annot. missing: 3 (75%)RXN0-6368, H2NTPEPIM-RXN, RXN0-6367 Unpredicted: 0 Entire module: 1 (25%)GTP-CYCLOHYDRO-I-RXN Blocked rxns: 0 Essential rxns: 1GTP-CYCLOHYDRO-I-RXN	Annotated: 1 (100%)1.1.1.83-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)1.1.1.83-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)ALDOSE-1-EPIMERASE-RXN, TREHALA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)RXN0-6444, RXN0-6460, RXN-8974, RXN0-6452, RXN0-5222 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (100%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN, ETHAMLY-RXN, PHOSACETYLTRANS-RXN, ACETATEKIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)ALCOHOL-DEHYDROG-RXN, ACETALD-DEHYDROG-RXN, ETHAMLY-RXN, PHOSACETYLTRANS-RXN, ACETATEKIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (67%)DAPASYN-RXN, DETHIOBIOTIN-SYN-RXN Annot. missing: 1 (33%)2.8.1.6-RXN Unpredicted: 0 Entire module: 2 (67%)DAPASYN-RXN, DETHIOBIOTIN-SYN-RXN Blocked rxns: 2DAPASYN-RXN, DETHIOBIOTIN-SYN-RXN Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)SEDOBISALDOL-RXN, RXN0-6541 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	curcumin degradation	methylphosphonate degradation I	hydrogen sulfide biosynthesis I	D-serine degradation	proline to cytochrome bo oxidase electron transfer	cardiolipin biosynthesis III	muropeptide degradation	5-(carboxymethoxy)uridine biosynthesis	glycerol-3-phosphate to cytochrome bo oxidase electron transfer	D-lactate to cytochrome bo oxidase electron transfer
e_coli_core	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-6677, RXN0-6676 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN0-6732, RXN0-6734, RXN0-6733, RXN0-6710 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)CYSTEINE-AMINOTRANSFERASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-15581, RXN-15124, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-7008, RXN0-5268 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)PHOSPHAGLYPSYN-RXN, RXN0-7012, PGPPHOSPHA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN0-961, RXN0-5227, RXN0-6981, RXN0-5228 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-7067, RXN0-7066 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5260, RXN0-5268 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)DLACTDEHYDROGFAD-RXN, RXN0-5268 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-6677, RXN0-6676 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RXN0-6732, RXN0-6734, RXN0-6733, RXN0-6710 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)CYSTEINE-AMINOTRANSFERASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-15581, RXN-15124, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-7008, RXN0-5268 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)PHOSPHAGLYPSYN-RXN, RXN0-7012, PGPPHOSPHA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (50%)RXN0-6981, RXN0-5228 Annot. missing: 2 (50%)RXN0-961, RXN0-5227 Unpredicted: 0 Entire module: 2 (50%)RXN0-6981, RXN0-5228 Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-7067, RXN0-7066 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5260, RXN0-5268 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)DLACTDEHYDROGFAD-RXN, RXN0-5268 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	NADH to cytochrome bo oxidase electron transfer II	NADH to cytochrome bd oxidase electron transfer II	autoinducer AI-2 degradation	nitrate reduction VIIIb (dissimilatory)	hydrogen to fumarate electron transfer	hydrogen to dimethyl sulfoxide electron transfer	hydrogen to trimethylamine N-oxide electron transfer	nitrate reduction IX (dissimilatory)	glycerol-3-phosphate to fumarate electron transfer	nitrate reduction X (dissimilatory, periplasmic)
e_coli_core	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5330, RXN0-5268 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5266, RXN0-5330 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-5461, RXN-15216, RXN-15943 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-7124, RXN0-5330 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)R601-RXN, RXN0-5256 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5256, DIMESULFREDUCT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5256, RXN0-5264 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-3501, RXN-15740 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)R601-RXN, RXN-15740 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-5260, RXN-18584, NITRATE-REDUCTASE-CYTOCHROME-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5330, RXN0-5268 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5266, RXN0-5330 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-5461, RXN-15216, RXN-15943 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-7124, RXN0-5330 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)R601-RXN, RXN0-5256 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5256, DIMESULFREDUCT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5256, RXN0-5264 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-3501, RXN-15740 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)R601-RXN, RXN-15740 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-5260, RXN-18584, NITRATE-REDUCTASE-CYTOCHROME-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	formate to nitrite electron transfer	peptidoglycan maturation (meso-diaminopimelate containing)	N⁶-L-threonylcarbamoyladenosine³⁷-modified tRNA biosynthesis	NADH to hydrogen peroxide electron transfer	glycerol-3-phosphate to hydrogen peroxide electron transport	superpathway of pyrimidine ribonucleotides de novo biosynthesis	superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)	L-ascorbate degradation I (bacterial, anaerobic)	phenylacetate degradation I (aerobic)	glycerol and glycerophosphodiester degradation
e_coli_core	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-18604, RXN-18605, FORMATEDEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 12 (100%)RXN-16685, RXN-16650, RXN-16659, RXN-16649, RXN-16691, RXN-16660, RXN-16687, RXN-16664, RXN-16690, RXN-16688, RXN-16665, RXN-16689 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN-14570, RXN-14569 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5388, RXN-19020 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN-15740, RXN-19020 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 9 (100%)CARBPSYN-RXN, DIHYDROOROT-RXN, DIHYDROOROTATE-DEHYDROGENASE-RXN, ASPCARBTRANS-RXN, OROPRIBTRANS-RXN, RXN-12002, UDPKIN-RXN, CTPSYN-RXN, OROTPDECARB-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 14 (100%)UDPREDUCT-RXN, RIBONUCLEOSIDE-DIP-REDUCTII-RXN, DUDPKIN-RXN, RXN0-724, DTMPKI-RXN, DUTP-PYROP-RXN, THYMIDYLATESYN-RXN, RXN0-722, RXN-12195, CDPREDUCT-RXN, DCDPKIN-RXN, DTDPKIN-RXN, RXN0-723, DCTP-DEAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)RIBULPEPIM-RXN, RXN0-5214, LXULRU5P-RXN, RXN0-705 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 9 (100%)RXN0-2042, RXNMETA-12672, RXN-10819, RXN-3641, RXN0-2044, RXN-2425, RXN0-6510, RXN0-6512, RXNMETA-12671 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)GLYCPDIESTER-RXN, GLYCEROL-KIN-RXN, RXN0-5260, RXN-15740 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-18604, RXN-18605, FORMATEDEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 12 (100%)RXN-16685, RXN-16650, RXN-16659, RXN-16649, RXN-16691, RXN-16660, RXN-16687, RXN-16664, RXN-16690, RXN-16688, RXN-16665, RXN-16689 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN-14570, RXN-14569 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-5388, RXN-19020 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN-15740, RXN-19020 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 8 (89%)CARBPSYN-RXN, DIHYDROOROT-RXN, ASPCARBTRANS-RXN, OROPRIBTRANS-RXN, RXN-12002, UDPKIN-RXN, CTPSYN-RXN, OROTPDECARB-RXN Annot. missing: 1 (11%)DIHYDROOROTATE-DEHYDROGENASE-RXN Unpredicted: 0 Entire module: 8 (89%)CARBPSYN-RXN, DIHYDROOROT-RXN, ASPCARBTRANS-RXN, OROPRIBTRANS-RXN, RXN-12002, UDPKIN-RXN, CTPSYN-RXN, OROTPDECARB-RXN Blocked rxns: 0 Essential rxns: 7ASPCARBTRANS-RXN, DIHYDROOROT-RXN, OROPRIBTRANS-RXN, RXN-12002, UDPKIN-RXN, CTPSYN-RXN, OROTPDECARB-RXN	Annotated: 9 (64%)UDPREDUCT-RXN, DUDPKIN-RXN, DTMPKI-RXN, DUTP-PYROP-RXN, RXN-12195, CDPREDUCT-RXN, DCDPKIN-RXN, DTDPKIN-RXN, DCTP-DEAM-RXN Annot. missing: 5 (36%)RIBONUCLEOSIDE-DIP-REDUCTII-RXN, RXN0-724, RXN0-722, THYMIDYLATESYN-RXN, RXN0-723 Unpredicted: 0 Entire module: 9 (64%)UDPREDUCT-RXN, DUDPKIN-RXN, DTMPKI-RXN, DUTP-PYROP-RXN, RXN-12195, DCDPKIN-RXN, CDPREDUCT-RXN, DTDPKIN-RXN, DCTP-DEAM-RXN Blocked rxns: 0 Essential rxns: 2DTMPKI-RXN, DTDPKIN-RXN	Annotated: 4 (100%)RIBULPEPIM-RXN, RXN0-5214, LXULRU5P-RXN, RXN0-705 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)RIBULPEPIM-RXN, RXN0-5214, LXULRU5P-RXN, RXN0-705 Blocked rxns: 2RXN0-5214, RXN0-705 Essential rxns: 0	Annotated: 1 (11%)RXN-10819 Annot. missing: 8 (89%)RXN0-2042, RXNMETA-12672, RXN-3641, RXN-2425, RXN0-2044, RXN0-6510, RXN0-6512, RXNMETA-12671 Unpredicted: 0 Entire module: 1 (11%)RXN-10819 Blocked rxns: 1RXN-10819 Essential rxns: 0	Annotated: 1 (25%)GLYCEROL-KIN-RXN Annot. missing: 3 (75%)GLYCPDIESTER-RXN, RXN0-5260, RXN-15740 Unpredicted: 0 Entire module: 1 (33%)GLYCEROL-KIN-RXN Blocked rxns: 0 Essential rxns: 0

	allantoin degradation IV (anaerobic)	2-methylcitrate cycle I	conversion of succinate to propanoate	D-allose degradation	L-lysine degradation I	lipoate biosynthesis and incorporation I	fructoselysine and psicoselysine degradation	lipoate salvage I	cyclopropane fatty acid (CFA) biosynthesis	PRPP biosynthesis
e_coli_core	Annotated: 0 (0%) Annot. missing: 8 (100%)OXAMATE-CARBAMOYLTRANSFERASE-RXN, RXN-14196, R165-RXN, ALLANTOINASE-RXN, ALLANTOATE-DEIMINASE-RXN, URUR-RXN, RXN0-7024, RXN0-5222 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)4.2.1.99-RXN, METHYLISOCITRATE-LYASE-RXN, PROPIONATE--COA-LIGASE-RXN, 2-METHYLCITRATE-SYNTHASE-RXN, 2-METHYLCITRATE-DEHYDRATASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-268, RXN0-310, METHYLMALONYL-COA-MUT-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-304, RXN0-303, ALLOSE-KINASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)LYSDECARBOX-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-947, RXN0-949 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-4841, RXN0-962, RXN0-963 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN-8654, RXN-8655 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)2.1.1.79-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)PRPPSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 2 (25%)OXAMATE-CARBAMOYLTRANSFERASE-RXN, RXN0-7024 Annot. missing: 6 (75%)RXN-14196, R165-RXN, ALLANTOINASE-RXN, ALLANTOATE-DEIMINASE-RXN, URUR-RXN, RXN0-5222 Unpredicted: 0 Entire module: 2 (25%)OXAMATE-CARBAMOYLTRANSFERASE-RXN, RXN0-7024 Blocked rxns: 0 Essential rxns: 0	Annotated: 4 (80%)2-METHYLCITRATE-SYNTHASE-RXN, METHYLISOCITRATE-LYASE-RXN, 2-METHYLCITRATE-DEHYDRATASE-RXN, 4.2.1.99-RXN Annot. missing: 1 (20%)PROPIONATE--COA-LIGASE-RXN Unpredicted: 0 Entire module: 4 (80%)2-METHYLCITRATE-SYNTHASE-RXN, METHYLISOCITRATE-LYASE-RXN, 2-METHYLCITRATE-DEHYDRATASE-RXN, 4.2.1.99-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (33%)METHYLMALONYL-COA-MUT-RXN Annot. missing: 2 (67%)RXN0-268, RXN0-310 Unpredicted: 0 Entire module: 1 (33%)METHYLMALONYL-COA-MUT-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)RXN0-304, RXN0-303, ALLOSE-KINASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)RXN0-304, RXN0-303, ALLOSE-KINASE-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)LYSDECARBOX-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)LYSDECARBOX-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN0-947, RXN0-949 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (33%)RXN0-4841 Annot. missing: 2 (67%)RXN0-962, RXN0-963 Unpredicted: 0 Entire module: 1 (33%)RXN0-4841 Blocked rxns: 1RXN0-4841 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RXN-8654, RXN-8655 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)2.1.1.79-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)PRPPSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	aspartate superpathway	L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	superpathway of pyridoxal 5'-phosphate biosynthesis and salvage	(5Z)-dodecenoate biosynthesis I	superpathway of fatty acid biosynthesis I (E. coli)	taurine degradation IV	stearate biosynthesis III (fungi)	NAD salvage pathway IV	ethanol degradation II	gluconeogenesis III
e_coli_core	Annotated: 0 (0%) Annot. missing: 27 (100%)QUINOPRIBOTRANS-RXN, O-SUCCHOMOSERLYASE-RXN, L-ASPARTATE-OXID-RXN, HOMOSERDEHYDROG-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, DIHYDRODIPICSYN-RXN, RXN-14014, ASPAMINOTRANS-RXN, HOMOCYSMETB12-RXN, HOMOCYSMET-RXN, S-ADENMETSYN-RXN, RXN-15124, ASPARTATEKIN-RXN, DIAMINOPIMDECARB-RXN, SUCCINYLDIAMINOPIMTRANS-RXN, QUINOLINATE-SYNTHA-RXN, NICONUCADENYLYLTRAN-RXN, RXN-15127, THRESYN-RXN, HOMOSERKIN-RXN, NAD-SYNTH-NH3-RXN, TETHYDPICSUCC-RXN, HOMSUCTRAN-RXN, NAD-SYNTH-GLN-RXN, SUCCDIAMINOPIMDESUCC-RXN, RXN-15131, DIAMINOPIMEPIM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)ARGDECARBOX-RXN, AGMATIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 11 (100%)RXN-13179, DXS-RXN, ERYTHRON4PDEHYDROG-RXN, ERYTH4PDEHYDROG-RXN, PSERTRANSAMPYR-RXN, PYRIDOXKIN-RXN, PYRAMKIN-RXN, PNPOXI-RXN, PNKIN-RXN, PDXJ-RXN, PMPOXI-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)RXN0-2142, RXN0-2141, RXN0-2144, RXN0-2145, RXN-9655, 5.3.3.14-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 17 (100%)3-OXOACYL-ACP-REDUCT-RXN, RXN0-2142, ACP-S-ACETYLTRANSFER-RXN, RXN0-2141, 2.3.1.180-RXN, 3-OXOACYL-ACP-SYNTH-RXN, RXN0-2144, MALONYL-COA-ACP-TRANSACYL-RXN, RXN0-2145, MALONYL-ACPDECARBOX-RXN, 3-HYDROXYDECANOYL-ACP-DEHYDR-RXN, RXN0-5055, ENOYL-ACP-REDUCT-NADH-RXN, RXN-9655, ENOYL-ACP-REDUCT-NADPH-RXN, 5.3.3.14-RXN, 3-OXOACYL-ACP-SYNTH-BASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)RXN0-299 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)RIBOSYLNICOTINAMIDE-KINASE-RXN, 2.7.7.1-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 1 (33%)ALCOHOL-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Entire module: 9 (75%)GAPOXNPHOSPHN-RXN, RXN-15513, F16BDEPHOS-RXN, PGLUCISOM-RXN, PHOSGLYPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, MALATE-DEH-RXN Blocked rxns: 0 Essential rxns: 42PGADEHYDRAT-RXN, GAPOXNPHOSPHN-RXN, PHOSGLYPHOS-RXN, RXN-15513
iAF1260	Annotated: 18 (67%)NICONUCADENYLYLTRAN-RXN, S-ADENMETSYN-RXN, THRESYN-RXN, HOMOSERKIN-RXN, ASPARTATEKIN-RXN, QUINOPRIBOTRANS-RXN, O-SUCCHOMOSERLYASE-RXN, NAD-SYNTH-NH3-RXN, L-ASPARTATE-OXID-RXN, DIAMINOPIMDECARB-RXN, TETHYDPICSUCC-RXN, HOMSUCTRAN-RXN, ASPAMINOTRANS-RXN, SUCCDIAMINOPIMDESUCC-RXN, DIAMINOPIMEPIM-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCINYLDIAMINOPIMTRANS-RXN, QUINOLINATE-SYNTHA-RXN Annot. missing: 9 (33%)RXN-15124, HOMOSERDEHYDROG-RXN, HOMOCYSMETB12-RXN, RXN-14014, NAD-SYNTH-GLN-RXN, RXN-15131, DIHYDRODIPICSYN-RXN, HOMOCYSMET-RXN, RXN-15127 Unpredicted: 0 Entire module: 18 (67%)NICONUCADENYLYLTRAN-RXN, S-ADENMETSYN-RXN, THRESYN-RXN, HOMOSERKIN-RXN, ASPARTATEKIN-RXN, QUINOPRIBOTRANS-RXN, O-SUCCHOMOSERLYASE-RXN, NAD-SYNTH-NH3-RXN, L-ASPARTATE-OXID-RXN, DIAMINOPIMDECARB-RXN, TETHYDPICSUCC-RXN, HOMSUCTRAN-RXN, ASPAMINOTRANS-RXN, SUCCDIAMINOPIMDESUCC-RXN, DIAMINOPIMEPIM-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCINYLDIAMINOPIMTRANS-RXN, QUINOLINATE-SYNTHA-RXN Blocked rxns: 0 Essential rxns: 17NICONUCADENYLYLTRAN-RXN, S-ADENMETSYN-RXN, THRESYN-RXN, HOMOSERKIN-RXN, ASPARTATEKIN-RXN, QUINOPRIBOTRANS-RXN, O-SUCCHOMOSERLYASE-RXN, NAD-SYNTH-NH3-RXN, TETHYDPICSUCC-RXN, DIAMINOPIMDECARB-RXN, HOMSUCTRAN-RXN, SUCCDIAMINOPIMDESUCC-RXN, ASPAMINOTRANS-RXN, DIAMINOPIMEPIM-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN, SUCCINYLDIAMINOPIMTRANS-RXN, QUINOLINATE-SYNTHA-RXN	Annotated: 2 (100%)ARGDECARBOX-RXN, AGMATIN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)ARGDECARBOX-RXN, AGMATIN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 9 (82%)DXS-RXN, ERYTHRON4PDEHYDROG-RXN, PSERTRANSAMPYR-RXN, PYRIDOXKIN-RXN, PYRAMKIN-RXN, PNPOXI-RXN, PNKIN-RXN, ERYTH4PDEHYDROG-RXN, PMPOXI-RXN Annot. missing: 2 (18%)PDXJ-RXN, RXN-13179 Unpredicted: 0 Entire module: 9 (82%)DXS-RXN, ERYTHRON4PDEHYDROG-RXN, PSERTRANSAMPYR-RXN, PYRIDOXKIN-RXN, PYRAMKIN-RXN, PNPOXI-RXN, PNKIN-RXN, ERYTH4PDEHYDROG-RXN, PMPOXI-RXN Blocked rxns: 0 Essential rxns: 4PSERTRANSAMPYR-RXN, ERYTH4PDEHYDROG-RXN, DXS-RXN, ERYTHRON4PDEHYDROG-RXN	Annotated: 0 (0%) Annot. missing: 6 (100%)RXN0-2142, RXN0-2141, RXN0-2144, RXN0-2145, RXN-9655, 5.3.3.14-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (29%)ACP-S-ACETYLTRANSFER-RXN, 2.3.1.180-RXN, MALONYL-ACPDECARBOX-RXN, MALONYL-COA-ACP-TRANSACYL-RXN, 3-OXOACYL-ACP-SYNTH-BASE-RXN Annot. missing: 12 (71%)3-OXOACYL-ACP-REDUCT-RXN, RXN0-2142, RXN0-2141, 3-OXOACYL-ACP-SYNTH-RXN, RXN0-2144, RXN0-2145, 3-HYDROXYDECANOYL-ACP-DEHYDR-RXN, RXN0-5055, ENOYL-ACP-REDUCT-NADH-RXN, RXN-9655, ENOYL-ACP-REDUCT-NADPH-RXN, 5.3.3.14-RXN Unpredicted: 0 Entire module: 5 (29%)ACP-S-ACETYLTRANSFER-RXN, 2.3.1.180-RXN, MALONYL-ACPDECARBOX-RXN, MALONYL-COA-ACP-TRANSACYL-RXN, 3-OXOACYL-ACP-SYNTH-BASE-RXN Blocked rxns: 0 Essential rxns: 1MALONYL-COA-ACP-TRANSACYL-RXN	Annotated: 1 (100%)RXN0-299 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)RXN0-299 Blocked rxns: 0 Essential rxns: 0	Unpredicted: 2RXN-9634, RXN-9624 Entire module: 2 (33%)RXN-9634, RXN-9624 Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (50%)2.7.7.1-RXN Annot. missing: 1 (50%)RIBOSYLNICOTINAMIDE-KINASE-RXN Unpredicted: 0 Entire module: 1 (50%)2.7.7.1-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1RXN66-3 Entire module: 3 (100%)ALCOHOL-DEHYDROG-RXN, ACETATE--COA-LIGASE-RXN, RXN66-3 Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1RXN66-526 Entire module: 10 (83%)GAPOXNPHOSPHN-RXN, RXN-15513, F16BDEPHOS-RXN, RXN66-526, PGLUCISOM-RXN, PHOSGLYPHOS-RXN, TRIOSEPISOMERIZATION-RXN, F16ALDOLASE-RXN, 2PGADEHYDRAT-RXN, MALATE-DEH-RXN Blocked rxns: 0 Essential rxns: 0

	superpathway of purine nucleotide salvage	D-galactose degradation V (Leloir pathway)	hydrogen sulfide biosynthesis II (mammalian)	L-threonine degradation V	dopamine degradation	NAD salvage pathway I	NAD biosynthesis I (from aspartate)	pyridoxal 5'-phosphate biosynthesis I	pyruvate decarboxylation to acetyl CoA	L-rhamnose degradation I
e_coli_core	Entire module: 1 (6%)ADENYL-KIN-RXN Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)NICONUCADENYLYLTRAN-RXN, NMNNUCLEOSID-RXN, NICOTINATEPRIBOSYLTRANS-RXN, NADPYROPHOSPHAT-RXN, NAD-SYNTH-GLN-RXN, NICOTINAMID-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)NICONUCADENYLYLTRAN-RXN, QUINOPRIBOTRANS-RXN, NAD-SYNTH-NH3-RXN, L-ASPARTATE-OXID-RXN, NAD-SYNTH-GLN-RXN, QUINOLINATE-SYNTHA-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 7 (100%)RXN-13179, DXS-RXN, ERYTHRON4PDEHYDROG-RXN, ERYTH4PDEHYDROG-RXN, PSERTRANSAMPYR-RXN, PNPOXI-RXN, PDXJ-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-1133, RXN0-1134, RXN0-1132 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)RXN-15356, RHAMNULPALDOL-RXN, RHAMNULOKIN-RXN, RHAMNISOM-RXN, RXN0-5306 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Unpredicted: 1ADENOSINE-KINASE-RXN Entire module: 17 (100%)DADPKIN-RXN, GDPKIN-RXN, ADPREDUCT-RXN, GUANYL-KIN-RXN, ADENYL-KIN-RXN, IMP-DEHYDROG-RXN, AMPSYN-RXN, GUANPRIBOSYLTRAN-RXN, ADENYLOSUCCINATE-SYNTHASE-RXN, ADENPHOSPHOR-RXN, INOPHOSPHOR-RXN, ADENPRIBOSYLTRAN-RXN, ADENODEAMIN-RXN, RXN0-5199, HYPOXANPRIBOSYLTRAN-RXN, GMP-SYN-GLUT-RXN, ADENOSINE-KINASE-RXN Blocked rxns: 0 Essential rxns: 4GMP-SYN-GLUT-RXN, ADENYLOSUCCINATE-SYNTHASE-RXN, GUANYL-KIN-RXN, AMPSYN-RXN	Unpredicted: 3GALACTURIDYLYLTRANS-RXN, ALDOSE1EPIM-RXN, GALACTOKIN-RXN Entire module: 5 (100%)GALACTURIDYLYLTRANS-RXN, ALDOSE1EPIM-RXN, UDPGLUCEPIM-RXN, PHOSPHOGLUCMUT-RXN, GALACTOKIN-RXN Blocked rxns: 1ALDOSE1EPIM-RXN Essential rxns: 0	Unpredicted: 1LCYSDESULF-RXN Entire module: 1 (17%)LCYSDESULF-RXN Blocked rxns: 0 Essential rxns: 0	Unpredicted: 1THREDEHYD-RXN Entire module: 1 (50%)THREDEHYD-RXN Blocked rxns: 0 Essential rxns: 1THREDEHYD-RXN	Unpredicted: 1RXN6666-4 Entire module: 1 (20%)RXN6666-4 Blocked rxns: 1RXN6666-4 Essential rxns: 0	Annotated: 4 (67%)NICONUCADENYLYLTRAN-RXN, NICOTINAMID-RXN, NADPYROPHOSPHAT-RXN, NICOTINATEPRIBOSYLTRANS-RXN Annot. missing: 2 (33%)NAD-SYNTH-GLN-RXN, NMNNUCLEOSID-RXN Unpredicted: 0 Entire module: 4 (67%)NICONUCADENYLYLTRAN-RXN, NICOTINAMID-RXN, NADPYROPHOSPHAT-RXN, NICOTINATEPRIBOSYLTRANS-RXN Blocked rxns: 0 Essential rxns: 1NICONUCADENYLYLTRAN-RXN	Annotated: 5 (83%)NICONUCADENYLYLTRAN-RXN, QUINOPRIBOTRANS-RXN, NAD-SYNTH-NH3-RXN, L-ASPARTATE-OXID-RXN, QUINOLINATE-SYNTHA-RXN Annot. missing: 1 (17%)NAD-SYNTH-GLN-RXN Unpredicted: 0 Entire module: 5 (83%)NICONUCADENYLYLTRAN-RXN, QUINOPRIBOTRANS-RXN, NAD-SYNTH-NH3-RXN, L-ASPARTATE-OXID-RXN, QUINOLINATE-SYNTHA-RXN Blocked rxns: 0 Essential rxns: 4NICONUCADENYLYLTRAN-RXN, NAD-SYNTH-NH3-RXN, QUINOPRIBOTRANS-RXN, QUINOLINATE-SYNTHA-RXN	Annotated: 5 (71%)DXS-RXN, ERYTHRON4PDEHYDROG-RXN, PSERTRANSAMPYR-RXN, PNPOXI-RXN, ERYTH4PDEHYDROG-RXN Annot. missing: 2 (29%)PDXJ-RXN, RXN-13179 Unpredicted: 0 Entire module: 5 (71%)DXS-RXN, ERYTHRON4PDEHYDROG-RXN, PSERTRANSAMPYR-RXN, PNPOXI-RXN, ERYTH4PDEHYDROG-RXN Blocked rxns: 0 Essential rxns: 4PSERTRANSAMPYR-RXN, ERYTH4PDEHYDROG-RXN, DXS-RXN, ERYTHRON4PDEHYDROG-RXN	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-1133, RXN0-1134, RXN0-1132 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (20%)RHAMNULPALDOL-RXN Annot. missing: 4 (80%)RHAMNISOM-RXN, RHAMNULOKIN-RXN, RXN0-5306, RXN-15356 Unpredicted: 0 Entire module: 1 (20%)RHAMNULPALDOL-RXN Blocked rxns: 0 Essential rxns: 0

	ribose phosphorylation	flavin biosynthesis I (bacteria and plants)	adenosine nucleotides degradation II	xanthine and xanthosine salvage	S-adenosyl-L-methionine biosynthesis	superpathway of L-serine and glycine biosynthesis I	L-serine degradation	L-serine biosynthesis	sulfate reduction I (assimilatory)	D-sorbitol degradation II
e_coli_core	Annotated: 0 (0%) Annot. missing: 2 (100%)RIBOKIN-RXN, RXN0-5304 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 9 (100%)RIBOFLAVINSYNREDUC-RXN, LUMAZINESYN-RXN, RIBOPHOSPHAT-RXN, DIOHBUTANONEPSYN-RXN, FADSYN-RXN, RIBOFLAVIN-SYN-RXN, RIBOFLAVINSYNDEAM-RXN, GTP-CYCLOHYDRO-II-RXN, RIBOFLAVINKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 5 (100%)AMP-DEPHOSPHORYLATION-RXN, RXN-7682, INOPHOSPHOR-RXN, ADENODEAMIN-RXN, RXN0-901 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)XANTHOSINEPHOSPHORY-RXN, XANPRIBOSYLTRAN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)S-ADENMETSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)PGLYCDEHYDROG-RXN, GLYOHMETRANS-RXN, PSERTRANSAM-RXN, RXN0-5114 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-15125, RXN-15124, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN0-5114, PGLYCDEHYDROG-RXN, PSERTRANSAM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)ADENYLYLSULFKIN-RXN, SULFITE-REDUCT-RXN, 1.8.4.8-RXN, SULFATE-ADENYLYLTRANS-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 1 (100%)SORB6PDEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 0 (0%) Annot. missing: 2 (100%)RIBOKIN-RXN, RXN0-5304 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 8 (89%)RIBOFLAVINSYNREDUC-RXN, RIBOPHOSPHAT-RXN, DIOHBUTANONEPSYN-RXN, FADSYN-RXN, RIBOFLAVIN-SYN-RXN, RIBOFLAVINSYNDEAM-RXN, GTP-CYCLOHYDRO-II-RXN, RIBOFLAVINKIN-RXN Annot. missing: 1 (11%)LUMAZINESYN-RXN Unpredicted: 0 Entire module: 8 (89%)RIBOFLAVINSYNREDUC-RXN, RIBOPHOSPHAT-RXN, DIOHBUTANONEPSYN-RXN, FADSYN-RXN, RIBOFLAVIN-SYN-RXN, RIBOFLAVINSYNDEAM-RXN, GTP-CYCLOHYDRO-II-RXN, RIBOFLAVINKIN-RXN Blocked rxns: 0 Essential rxns: 8RIBOFLAVINSYNREDUC-RXN, RIBOPHOSPHAT-RXN, DIOHBUTANONEPSYN-RXN, FADSYN-RXN, RIBOFLAVIN-SYN-RXN, RIBOFLAVINSYNDEAM-RXN, GTP-CYCLOHYDRO-II-RXN, RIBOFLAVINKIN-RXN	Annotated: 5 (100%)AMP-DEPHOSPHORYLATION-RXN, RXN-7682, INOPHOSPHOR-RXN, ADENODEAMIN-RXN, RXN0-901 Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)AMP-DEPHOSPHORYLATION-RXN, RXN-7682, INOPHOSPHOR-RXN, ADENODEAMIN-RXN, RXN0-901 Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (100%)XANTHOSINEPHOSPHORY-RXN, XANPRIBOSYLTRAN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)XANTHOSINEPHOSPHORY-RXN, XANPRIBOSYLTRAN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (100%)S-ADENMETSYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 1 (100%)S-ADENMETSYN-RXN Blocked rxns: 0 Essential rxns: 1S-ADENMETSYN-RXN	Annotated: 3 (75%)PGLYCDEHYDROG-RXN, PSERTRANSAM-RXN, RXN0-5114 Annot. missing: 1 (25%)GLYOHMETRANS-RXN Unpredicted: 0 Entire module: 3 (75%)PGLYCDEHYDROG-RXN, PSERTRANSAM-RXN, RXN0-5114 Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-15125, RXN-15124, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)RXN0-5114, PGLYCDEHYDROG-RXN, PSERTRANSAM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)RXN0-5114, PGLYCDEHYDROG-RXN, PSERTRANSAM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (75%)ADENYLYLSULFKIN-RXN, SULFITE-REDUCT-RXN, 1.8.4.8-RXN Annot. missing: 1 (25%)SULFATE-ADENYLYLTRANS-RXN Unpredicted: 0 Entire module: 3 (75%)ADENYLYLSULFKIN-RXN, SULFITE-REDUCT-RXN, 1.8.4.8-RXN Blocked rxns: 0 Essential rxns: 2SULFITE-REDUCT-RXN, ADENYLYLSULFKIN-RXN	Annotated: 0 (0%) Annot. missing: 1 (100%)SORB6PDEHYDROG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0

	superpathway of sulfate assimilation and cysteine biosynthesis	TCA cycle I (prokaryotic)	superpathway of glyoxylate bypass and TCA	thioredoxin pathway	superpathway of thiamine diphosphate biosynthesis I	L-threonine degradation III (to methylglyoxal)	superpathway of L-threonine metabolism	L-threonine degradation II	superpathway of L-threonine biosynthesis	trehalose degradation I (low osmolarity)
e_coli_core	Annotated: 0 (0%) Annot. missing: 9 (100%)SULFITE-REDUCT-RXN, PSERTRANSAM-RXN, 1.8.4.8-RXN, SULFATE-ADENYLYLTRANS-RXN, PGLYCDEHYDROG-RXN, RXN0-5114, SERINE-O-ACETTRAN-RXN, ACSERLY-RXN, ADENYLYLSULFKIN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (60%)CITSYN-RXN, ACONITATEDEHYDR-RXN, FUMHYDR-RXN, 2OXOGLUTARATEDEH-RXN, SUCCCOASYN-RXN, MALATE-DEH-RXN Annot. missing: 4 (40%)SUCCINATE-DEHYDROGENASE-UBIQUINONE-RXN, ISOCITDEH-RXN, ACONITATEHYDR-RXN, MALATE-DEHYDROGENASE-ACCEPTOR-RXN Unpredicted: 0 Entire module: 6 (60%)CITSYN-RXN, ACONITATEDEHYDR-RXN, FUMHYDR-RXN, 2OXOGLUTARATEDEH-RXN, SUCCCOASYN-RXN, MALATE-DEH-RXN Blocked rxns: 0 Essential rxns: 2CITSYN-RXN, ACONITATEDEHYDR-RXN	Annotated: 7 (58%)2OXOGLUTARATEDEH-RXN, CITSYN-RXN, ACONITATEDEHYDR-RXN, FUMHYDR-RXN, MALSYN-RXN, SUCCCOASYN-RXN, MALATE-DEH-RXN Annot. missing: 5 (42%)ISOCIT-CLEAV-RXN, MALATE-DEHYDROGENASE-ACCEPTOR-RXN, SUCCINATE-DEHYDROGENASE-UBIQUINONE-RXN, ACONITATEHYDR-RXN, ISOCITDEH-RXN Unpredicted: 0 Entire module: 7 (58%)2OXOGLUTARATEDEH-RXN, CITSYN-RXN, ACONITATEDEHYDR-RXN, FUMHYDR-RXN, MALSYN-RXN, SUCCCOASYN-RXN, MALATE-DEH-RXN Blocked rxns: 0 Essential rxns: 2CITSYN-RXN, ACONITATEDEHYDR-RXN	Annotated: 0 (0%) Annot. missing: 2 (100%)THIOREDOXIN-RXN, THIOREDOXIN-REDUCT-NADPH-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 11 (100%)PYRIMSYN3-RXN, RXN-9788, DXS-RXN, RXN-12611, PYRIMSYN1-RXN, THIAZOLSYN2-RXN, THI-P-KIN-RXN, RXN-14382, RXN0-308, RXN-9789, RXN-11319 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)AMACETOXID-RXN, THREODEHYD-RXN, THREOSPON-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (6%)ACETALD-DEHYDROG-RXN Annot. missing: 15 (94%)PTAALT-RXN, RXN-15122, RXN-15121, ACETOOHBUTREDUCTOISOM-RXN, RXN-15123, AKBLIG-RXN, DIHYDROXYMETVALDEHYDRAT-RXN, THREONINE-ALDOLASE-RXN, THREOSPON-RXN, AMACETOXID-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN, THREODEHYD-RXN, PROPKIN-RXN, ACETOOHBUTSYN-RXN, KETOBUTFORMLY-RXN Unpredicted: 0 Entire module: 1 (6%)ACETALD-DEHYDROG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)THREODEHYD-RXN, AKBLIG-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)THRESYN-RXN, HOMOSERKIN-RXN, ASPARTATEKIN-RXN, HOMOSERDEHYDROG-RXN, ASPAMINOTRANS-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)GLUCOKIN-RXN, TRE6PHYDRO-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 8 (89%)SULFITE-REDUCT-RXN, PSERTRANSAM-RXN, 1.8.4.8-RXN, PGLYCDEHYDROG-RXN, RXN0-5114, SERINE-O-ACETTRAN-RXN, ACSERLY-RXN, ADENYLYLSULFKIN-RXN Annot. missing: 1 (11%)SULFATE-ADENYLYLTRANS-RXN Unpredicted: 0 Entire module: 8 (89%)SULFITE-REDUCT-RXN, PSERTRANSAM-RXN, 1.8.4.8-RXN, PGLYCDEHYDROG-RXN, RXN0-5114, SERINE-O-ACETTRAN-RXN, ACSERLY-RXN, ADENYLYLSULFKIN-RXN Blocked rxns: 0 Essential rxns: 4ADENYLYLSULFKIN-RXN, SERINE-O-ACETTRAN-RXN, SULFITE-REDUCT-RXN, ACSERLY-RXN	Annotated: 6 (60%)CITSYN-RXN, ACONITATEDEHYDR-RXN, FUMHYDR-RXN, 2OXOGLUTARATEDEH-RXN, SUCCCOASYN-RXN, MALATE-DEH-RXN Annot. missing: 4 (40%)SUCCINATE-DEHYDROGENASE-UBIQUINONE-RXN, ISOCITDEH-RXN, ACONITATEHYDR-RXN, MALATE-DEHYDROGENASE-ACCEPTOR-RXN Unpredicted: 0 Entire module: 6 (60%)CITSYN-RXN, ACONITATEDEHYDR-RXN, FUMHYDR-RXN, 2OXOGLUTARATEDEH-RXN, SUCCCOASYN-RXN, MALATE-DEH-RXN Blocked rxns: 0 Essential rxns: 2CITSYN-RXN, ACONITATEDEHYDR-RXN	Annotated: 7 (58%)2OXOGLUTARATEDEH-RXN, CITSYN-RXN, ACONITATEDEHYDR-RXN, FUMHYDR-RXN, MALSYN-RXN, SUCCCOASYN-RXN, MALATE-DEH-RXN Annot. missing: 5 (42%)ISOCIT-CLEAV-RXN, MALATE-DEHYDROGENASE-ACCEPTOR-RXN, SUCCINATE-DEHYDROGENASE-UBIQUINONE-RXN, ACONITATEHYDR-RXN, ISOCITDEH-RXN Unpredicted: 0 Entire module: 7 (58%)2OXOGLUTARATEDEH-RXN, CITSYN-RXN, ACONITATEDEHYDR-RXN, FUMHYDR-RXN, MALSYN-RXN, SUCCCOASYN-RXN, MALATE-DEH-RXN Blocked rxns: 0 Essential rxns: 2CITSYN-RXN, ACONITATEDEHYDR-RXN	Annotated: 1 (50%)THIOREDOXIN-REDUCT-NADPH-RXN Annot. missing: 1 (50%)THIOREDOXIN-RXN Unpredicted: 0 Entire module: 1 (50%)THIOREDOXIN-REDUCT-NADPH-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 2 (18%)DXS-RXN, THI-P-KIN-RXN Annot. missing: 9 (82%)PYRIMSYN3-RXN, RXN-9788, RXN-12611, THIAZOLSYN2-RXN, PYRIMSYN1-RXN, RXN-14382, RXN0-308, RXN-9789, RXN-11319 Unpredicted: 0 Entire module: 2 (18%)DXS-RXN, THI-P-KIN-RXN Blocked rxns: 0 Essential rxns: 2DXS-RXN, THI-P-KIN-RXN	Annotated: 3 (100%)AMACETOXID-RXN, THREODEHYD-RXN, THREOSPON-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)AMACETOXID-RXN, THREODEHYD-RXN, THREOSPON-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 12 (75%)ACETALD-DEHYDROG-RXN, ACETOOHBUTREDUCTOISOM-RXN, AKBLIG-RXN, DIHYDROXYMETVALDEHYDRAT-RXN, THREONINE-ALDOLASE-RXN, THREOSPON-RXN, AMACETOXID-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN, THREODEHYD-RXN, PTAALT-RXN, ACETOOHBUTSYN-RXN, KETOBUTFORMLY-RXN Annot. missing: 4 (25%)RXN-15122, PROPKIN-RXN, RXN-15123, RXN-15121 Unpredicted: 0 Entire module: 12 (67%)ACETALD-DEHYDROG-RXN, ACETOOHBUTREDUCTOISOM-RXN, AKBLIG-RXN, DIHYDROXYMETVALDEHYDRAT-RXN, THREONINE-ALDOLASE-RXN, THREOSPON-RXN, AMACETOXID-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN, THREODEHYD-RXN, PTAALT-RXN, ACETOOHBUTSYN-RXN, KETOBUTFORMLY-RXN Blocked rxns: 0 Essential rxns: 4DIHYDROXYMETVALDEHYDRAT-RXN, ACETOOHBUTSYN-RXN, ACETOOHBUTREDUCTOISOM-RXN, BRANCHED-CHAINAMINOTRANSFERILEU-RXN	Annotated: 2 (100%)THREODEHYD-RXN, AKBLIG-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)THREODEHYD-RXN, AKBLIG-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 5 (83%)THRESYN-RXN, HOMOSERKIN-RXN, ASPARTATEKIN-RXN, ASPAMINOTRANS-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN Annot. missing: 1 (17%)HOMOSERDEHYDROG-RXN Unpredicted: 0 Entire module: 5 (83%)THRESYN-RXN, HOMOSERKIN-RXN, ASPARTATEKIN-RXN, ASPAMINOTRANS-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN Blocked rxns: 0 Essential rxns: 5THRESYN-RXN, HOMOSERKIN-RXN, ASPARTATEKIN-RXN, ASPAMINOTRANS-RXN, ASPARTATE-SEMIALDEHYDE-DEHYDROGENASE-RXN	Annotated: 2 (100%)GLUCOKIN-RXN, TRE6PHYDRO-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)GLUCOKIN-RXN, TRE6PHYDRO-RXN Blocked rxns: 0 Essential rxns: 0

	trehalose biosynthesis I	L-tryptophan biosynthesis	L-tryptophan degradation II (via pyruvate)	L-tyrosine biosynthesis I	superpathway of ubiquinol-8 biosynthesis (prokaryotic)	UDP-N-acetyl-D-glucosamine biosynthesis I	L-valine biosynthesis	xylose degradation I
e_coli_core	Annotated: 0 (0%) Annot. missing: 2 (100%)TREHALOSEPHOSPHA-RXN, TREHALOSE6PSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 6 (100%)PRTRANS-RXN, PRAISOM-RXN, RXN0-2382, RXN0-2381, IGPSYN-RXN, ANTHRANSYN-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-15578, RXN-15124, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 3 (100%)CHORISMATEMUT-RXN, PREPHENATEDEHYDROG-RXN, TYROSINE-AMINOTRANSFERASE-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 13 (100%)GPPSYN-RXN, 4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, FPPSYN-RXN, OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-RXN, 2-OCTAPRENYLPHENOL-HYDROX-RXN, RXN-8992, CHORPYRLY-RXN, 2-OCTAPRENYL-6-METHOXYPHENOL-HYDROX-RXN, 2-OCTAPRENYL-6-OHPHENOL-METHY-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, IPPISOM-RXN, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, DHHB-METHYLTRANSFER-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 1 (20%)PGLUCISOM-RXN Annot. missing: 4 (80%)5.4.2.10-RXN, L-GLN-FRUCT-6-P-AMINOTRANS-RXN, NAG1P-URIDYLTRANS-RXN, 2.3.1.157-RXN Unpredicted: 0 Entire module: 1 (20%)PGLUCISOM-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 4 (100%)ACETOLACTSYN-RXN, DIHYDROXYISOVALDEHYDRAT-RXN, ACETOLACTREDUCTOISOM-RXN, BRANCHED-CHAINAMINOTRANSFERVAL-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 0 (0%) Annot. missing: 2 (100%)XYLULOKIN-RXN, XYLISOM-RXN Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0
iAF1260	Annotated: 2 (100%)TREHALOSEPHOSPHA-RXN, TREHALOSE6PSYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)TREHALOSEPHOSPHA-RXN, TREHALOSE6PSYN-RXN Blocked rxns: 0 Essential rxns: 0	Annotated: 6 (100%)PRTRANS-RXN, PRAISOM-RXN, RXN0-2382, RXN0-2381, IGPSYN-RXN, ANTHRANSYN-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 6 (100%)PRTRANS-RXN, PRAISOM-RXN, RXN0-2382, RXN0-2381, IGPSYN-RXN, ANTHRANSYN-RXN Blocked rxns: 0 Essential rxns: 4PRTRANS-RXN, IGPSYN-RXN, PRAISOM-RXN, ANTHRANSYN-RXN	Annotated: 0 (0%) Annot. missing: 3 (100%)RXN-15578, RXN-15124, RXN-15127 Unpredicted: 0 Entire module: 0 (0%) Blocked rxns: 0 Essential rxns: 0	Annotated: 3 (100%)CHORISMATEMUT-RXN, PREPHENATEDEHYDROG-RXN, TYROSINE-AMINOTRANSFERASE-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 3 (100%)CHORISMATEMUT-RXN, PREPHENATEDEHYDROG-RXN, TYROSINE-AMINOTRANSFERASE-RXN Blocked rxns: 0 Essential rxns: 3CHORISMATEMUT-RXN, PREPHENATEDEHYDROG-RXN, TYROSINE-AMINOTRANSFERASE-RXN	Annotated: 10 (77%)GPPSYN-RXN, 4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, FPPSYN-RXN, CHORPYRLY-RXN, RXN-8992, 2-OCTAPRENYL-6-OHPHENOL-METHY-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, IPPISOM-RXN, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, DHHB-METHYLTRANSFER-RXN Annot. missing: 3 (23%)2-OCTAPRENYLPHENOL-HYDROX-RXN, 2-OCTAPRENYL-6-METHOXYPHENOL-HYDROX-RXN, OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-RXN Unpredicted: 0 Entire module: 10 (77%)GPPSYN-RXN, 4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, FPPSYN-RXN, CHORPYRLY-RXN, RXN-8992, 2-OCTAPRENYL-6-OHPHENOL-METHY-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, IPPISOM-RXN, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN, DHHB-METHYLTRANSFER-RXN Blocked rxns: 32-OCTAPRENYL-6-OHPHENOL-METHY-RXN, 2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN, DHHB-METHYLTRANSFER-RXN Essential rxns: 6GPPSYN-RXN, 4OHBENZOATE-OCTAPRENYLTRANSFER-RXN, FPPSYN-RXN, CHORPYRLY-RXN, RXN-8992, 3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN	Annotated: 5 (100%)2.3.1.157-RXN, 5.4.2.10-RXN, PGLUCISOM-RXN, NAG1P-URIDYLTRANS-RXN, L-GLN-FRUCT-6-P-AMINOTRANS-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 5 (100%)2.3.1.157-RXN, 5.4.2.10-RXN, PGLUCISOM-RXN, NAG1P-URIDYLTRANS-RXN, L-GLN-FRUCT-6-P-AMINOTRANS-RXN Blocked rxns: 0 Essential rxns: 45.4.2.10-RXN, L-GLN-FRUCT-6-P-AMINOTRANS-RXN, NAG1P-URIDYLTRANS-RXN, 2.3.1.157-RXN	Annotated: 4 (100%)ACETOLACTSYN-RXN, DIHYDROXYISOVALDEHYDRAT-RXN, ACETOLACTREDUCTOISOM-RXN, BRANCHED-CHAINAMINOTRANSFERVAL-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 4 (100%)ACETOLACTSYN-RXN, DIHYDROXYISOVALDEHYDRAT-RXN, ACETOLACTREDUCTOISOM-RXN, BRANCHED-CHAINAMINOTRANSFERVAL-RXN Blocked rxns: 0 Essential rxns: 3ACETOLACTSYN-RXN, ACETOLACTREDUCTOISOM-RXN, DIHYDROXYISOVALDEHYDRAT-RXN	Annotated: 2 (100%)XYLULOKIN-RXN, XYLISOM-RXN Annot. missing: 0 (0%) Unpredicted: 0 Entire module: 2 (100%)XYLULOKIN-RXN, XYLISOM-RXN Blocked rxns: 0 Essential rxns: 0

Are shown only BioCyc Pathways with four or more reactions, and at least 30% completion in superpathways, or 50% in regular pathways.

Growth of metabolic models is tested for 383 different culture media: 190 carbon sources, 95 nitrogen sources, 59 phosphorus sources, 35 sulfur sources, and 4 negative controls. 4 Biolog Phenotype Microarray Plates inspired the media selection below.
The color labels are:

Growth. The darker the purple, the higher is the flux through the objective function;

No growth;

The model has no reactions that can use the media-specific metabolite as a substrate, making the media growth phenotype identical to the negative control.

FBA fail.

Model: e_coli_core.xml
Objective reaction: BIOMASS_Ecoli_core_w_GAM
Extra metabolites, present in the objective reaction, included on every external media: []
Extra metabolites, not present in the objective reaction, included on every external media: ['co2 (carbon dioxide)', 'o2 (oxygen)']
Extra metabolites included in the external media when testing carbon sources: []
Extra metabolites included in the external media when testing nitrogen sources: []
Extra metabolites included in the external media when testing phosphorus sources: []
Extra metabolites included in the external media when testing sulfur sources: []

The inclusion of oxygen in the external media might have caused growth in a negative control media.

Overview about the growth phenotype analisis

No growth on negative control	Total growth	Growth without media-specific metabolites	Total no growth	No growth without media-specific metabolites	FBA failed	True positive (if *E. coli model)	True negative (if *E. coli model)	True negative without media-specific metabolites (if *E. coli model)	False positive (if *E. coli model)	False negative (if *E. coli model)	False negative without media-specific metabolites (if *E. coli model)	Phenotype accuracy (if *E. coli model)
3 out of 4	51	35	332	320	0	36	145	140	5	151	144	53.7 %

Growth phenotype on carbon sources

No growth on negative control	Total growth	Growth without media-specific metabolites	Total no growth	No growth without media-specific metabolites	FBA failed	True positive (if *E. coli model)	True negative (if *E. coli model)	True negative without media-specific metabolites (if *E. coli model)	False positive (if *E. coli model)	False negative (if *E. coli model)	False negative without media-specific metabolites (if *E. coli model)	Phenotype accuracy (if *E. coli model)
Yes	6	0	185	176	0	5	100	97	0	77	71	57.7 %

Growth phenotype on nitrogen sources

No growth on negative control	Total growth	Growth without media-specific metabolites	Total no growth	No growth without media-specific metabolites	FBA failed	True positive (if *E. coli model)	True negative (if *E. coli model)	True negative without media-specific metabolites (if *E. coli model)	False positive (if *E. coli model)	False negative (if *E. coli model)	False negative without media-specific metabolites (if *E. coli model)	Phenotype accuracy (if *E. coli model)
Yes	3	0	93	92	0	2	39	38	0	30	30	57.7 %

Growth phenotype on phosphorus sources

No growth on negative control	Total growth	Growth without media-specific metabolites	Total no growth	No growth without media-specific metabolites	FBA failed	True positive (if *E. coli model)	True negative (if *E. coli model)	True negative without media-specific metabolites (if *E. coli model)	False positive (if *E. coli model)	False negative (if *E. coli model)	False negative without media-specific metabolites (if *E. coli model)	Phenotype accuracy (if *E. coli model)
Yes	6	0	54	52	0	6	6	5	0	44	43	21.4 %

Growth phenotype on sulfur sources

No growth on negative control	Total growth	Growth without media-specific metabolites	Total no growth	No growth without media-specific metabolites	FBA failed	True positive (if *E. coli model)	True negative (if *E. coli model)	True negative without media-specific metabolites (if *E. coli model)	False positive (if *E. coli model)	False negative (if *E. coli model)	False negative without media-specific metabolites (if *E. coli model)	Phenotype accuracy (if *E. coli model)
No	36	35	0	0	0	23	0	0	5	0	0	82.1 %

*Escherichia coli K-12 substr. MG1655

Biolog Phenotype Microarray Plate 1 - Carbon Sources

	1	2	3	4	5	6	7	8	9	10	11	12
A	Optimization result: 0.0 (No growth) E. coli experimental result: No growth Carbon negative control	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Arabinose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth N-Acetyl-D- Glucosamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Saccharic acid	Optimization result: 0.0 (No growth) E. coli experimental result: Growth Succinic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Galactose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Aspartic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Proline	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Alanine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Trehalose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Mannose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Dulcitol
B	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Serine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Sorbitol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Glycerol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Fucose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Glucuronic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Gluconic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth DL-α- Glycerol Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Xylose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Lactic acid	Optimization result: 0.0 (No growth) E. coli experimental result: No growth Formic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Mannitol	Optimization result: 20.4736 (Growth) E. coli experimental result: Inconsistent results L-Glutamic acid
C	Optimization result: 41.9381 (Growth) E. coli experimental result: Growth D-Glucose- 6-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Galactonic acid-γ- Lactone	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth DL-Malic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Ribose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Tween 20	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Rhamnose	Optimization result: 35.044 (Growth) E. coli experimental result: Growth D-Fructose	Optimization result: 0.0 (No growth) E. coli experimental result: Growth Acetic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth α- D-Glucose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Maltose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Melibiose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Thymidine
D	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Asparagine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Aspartic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Glucosaminic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth 1,2-Propanediol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Tween 40	Optimization result: 27.1669 (Growth) E. coli experimental result: Growth α- Ketoglutaric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth α- Ketobutyric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth α- Methyl-D- Galactoside	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth α- D-Lactose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Lactulose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Sucrose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Uridine
E	Optimization result: 0.0 (No growth) E. coli experimental result: Low growth L-Glutamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth M-Tartaric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Glucose- 1-Phosphate	Optimization result: 41.9381 (Growth) E. coli experimental result: Growth D-Fructose- 6-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Tween 80	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth α- Hydroxyglutaric acid- γ-Lactone	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth α- Hydroxybutyric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth β- Methyl-D- Glucoside	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Adonitol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Maltotriose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth 2-Deoxyadenosine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Adenosine
F	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Gly-Asp	Optimization result: 0.0 (No growth) E. coli experimental result: No growth Citric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth M-Inositol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Threonine	Optimization result: 0.0 (No growth) E. coli experimental result: Growth Fumaric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Bromosuccinic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Propionic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Mucic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Glycolic acid	Optimization result: 0.0 (No growth) E. coli experimental result: Growth Glyoxylic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Cellobiose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Inosine
G	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Gly-Glu	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Tricarballylic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Serine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Threonine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Alanine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Ala-Gly	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Acetoacetic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth N-Acetyl-D- Mannosamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Low growth Mono-Methylsuccinate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Methylpyruvate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Malic acid	Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Malic acid
H	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Gly-Pro	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth p-Hydroxyphenyl Acetic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth m-Hydroxyphenyl Acetic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Tyramine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results D-Psicose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Lyxose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Glucuronamide	Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Pyruvic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Galactonic acid-γ- Lactone	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Galacturonic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Phenylethylamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth 2-Aminoethanol

Biolog Phenotype Microarray Plate 2 - Carbon Sources

	1	2	3	4	5	6	7	8	9	10	11	12
A		There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Chondroitin Sulfate C	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth α- Cyclodextrin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth β- Cyclodextrin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth γ- Cyclodextrin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Dextrin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Gelatin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Glycogen	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Inulin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Laminarin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Mannan	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Pectin
B	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth N-Acetyl-D- Galactosamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth N-Acetyl- Neuraminic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth β- D-Allose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Amygdalin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Arabinose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Arabitol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Arabitol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Arbutin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth 2-Deoxy- D-Ribose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth i-Erythritol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Fucose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth 3-O-β-D- Galactopyranosyl- D-Arabinose
C	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Gentiobiose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Glucose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Lactitol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Melezitose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Maltitol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results α- Methyl-D- Glucoside	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth β-Methyl- D-Galactoside	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth 3-Methylglucose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth β- Methyl-D- Glucuronic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth α- Methyl-D- Mannoside	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth β- Methyl-D- Xyloside	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Palatinose
D	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Raffinose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Salicin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Sedoheptulosan	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Sorbose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Stachyose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Tagatose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Turanose	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Xylitol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth N-Acetyl-D- Glucosaminitol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth γ- Amino-N- Butyric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth δ- Amino Valeric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Butyric acid
E	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Capric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Caproic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Citraconic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Citramalic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Glucosamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth 2-Hydroxybenzoic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth 4-Hydroxybenzoic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth β- Hydroxybutyric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth γ- Hydroxybutyric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results α- Keto- Valeric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Itaconic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth 5-Keto-D- Gluconic acid
F	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Lactic acid Methyl Ester	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Malonic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Melibionic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Oxalic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Oxalomalic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Quinic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Ribono- 1,4-Lactone	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Sebacic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Sorbic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Succinamic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Tartaric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Tartaric acid
G	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Acetamide	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results L-Alaninamide	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth N-Acetyl-L- Glutamic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Arginine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Glycine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Histidine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Homoserine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Hydroxy- L-Proline	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Isoleucine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Leucine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Lysine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Methionine
H	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Ornithine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Phenylalanine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Pyroglutamic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Valine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D,L-Carnitine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth sec-Butylamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D,L-Octopamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Putrescine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Dihydroxyacetone	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth 2,3-Butanediol	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth 2,3-Butanedione	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth 3-Hydroxy- 2-butanone

Biolog Phenotype Microarray Plate 3 - Nitrogen Sources

	1	2	3	4	5	6	7	8	9	10	11	12
A	Optimization result: 0.0 (No growth) E. coli experimental result: No growth nitrogen negative control	Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Ammonia	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Nitrite	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Nitrate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Urea	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Biuret	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Alanine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Arginine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Asparagine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Aspartic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results L-Cysteine	Optimization result: 26.72 (Growth) E. coli experimental result: Inconsistent results L-Glutamic acid
B	Optimization result: 24.113 (Growth) E. coli experimental result: Growth L-Glutamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Glycine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Histidine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Isoleucine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Leucine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results L-Lysine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results L-Methionine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results L-Phenylalanine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Proline	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Serine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results L-Threonine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Tryptophan
C	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Tyrosine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Valine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Alanine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results D-Asparagine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Aspartic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Glutamic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results D-Lysine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Serine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results D-Valine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Citrulline	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results L-Homoserine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth L-Ornithine
D	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth N-Acetyl-L- Glutamic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth N-Phthaloyl-L- Glutamic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth L-Pyroglutamic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Hydroxylamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Methylamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth N-Amylamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth N-Butylamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Ethylamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Ethanolamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Ethylenediamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Putrescine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Agmatine
E	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Histamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth b-Phenylethylamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Tyramine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Acetamide	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Formamide	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Glucuronamide	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth DL-Lactamide	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Glucosamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth D-Galactosamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results D-Mannosamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth N-Acetyl-D- Glucosamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth N-Acetyl-D- Galactosamine
F	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results N-Acetyl-D- Mannosamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Adenine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Adenosine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Cytidine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Cytosine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Guanine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Guanosine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Thymine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Thymidine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Uracil	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Uridine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Inosine
G	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Xanthine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Xanthosine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Uric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Alloxan	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Allantoin	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Parabanic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results DL-α- Amino-N- Butyric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results γ- Amino-N- Butyric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth ε- Amino-N- Caproic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results DL-α- Amino- Caprylic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth δ- Amino-N- Valeric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth α- Amino-N- Valeric acid
H	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Ala-Asp	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Ala-Gln	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Ala-Glu	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Ala-Gly	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Ala-His	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Ala-Leu	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Ala-Thr	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Gly-Asn	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Gly-Gln	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Gly-Glu	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Gly-Met	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Met-Ala

Biolog Phenotype Microarray Plate 4 - Phosphorus and Sulfur Sources

	1	2	3	4	5	6	7	8	9	10	11	12
A	Optimization result: 0.0 (No growth) E. coli experimental result: No growth phosphorus negative control	Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Pyrophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Trimetaphosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Tripolyphosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Triethyl Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Hypophosphite	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Adenosine 2'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Adenosine 3'- Monophosphate	Optimization result: 0.0 (No growth) E. coli experimental result: Growth Adenosine 5'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Adenosine 2', 3'-Cyclic Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Adenosine 3', 5'-Cyclic Monophosphate
B	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Thiophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Dithiophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth DL-α- Glycerol Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth β- Glycerol Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Carbamyl Phosphate	Optimization result: 38.2671 (Growth) E. coli experimental result: Growth D-2-Phospho- Glyceric acid	Optimization result: 38.2671 (Growth) E. coli experimental result: Growth D-3-Phospho- Glyceric acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Guanosine 2'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Guanosine 3'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Guanosine 5'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Guanosine 2', 3'-Cyclic Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Guanosine 3', 5'-Cyclic Monophosphate
C	Optimization result: 25.4756 (Growth) E. coli experimental result: Growth Phosphoenol Pyruvate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Phospho- Glycolic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Glucose- 1-Phosphate	Optimization result: 45.5028 (Growth) E. coli experimental result: Growth D-Glucose- 6-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth 2-Deoxy-D- Glucose 6- Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Glucosamine- 6-Phosphate	Optimization result: 48.0532 (Growth) E. coli experimental result: Growth 6-Phospho- Gluconic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Cytidine 2'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Cytidine 3'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Cytidine 5'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Cytidine 2', 3'-Cyclic Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Cytidine 3', 5'-Cyclic Monophosphate
D	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Mannose- 1-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth D-Mannose- 6-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Cysteamine- S-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Phospho- L-Arginine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth O-Phospho- D-Serine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth O-Phospho- L-Serine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth O-Phospho- L-Threonine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Uridine 2'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Uridine 3'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Uridine 5'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Uridine 2', 3'-Cyclic Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Uridine 3', 5'-Cyclic Monophosphate
E	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth O-Phospho- D-Tyrosine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth O-Phospho- L-Tyrosine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Phosphocreatine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Phosphoryl Choline	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth O-Phosphoryl- Ethanolamine	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Phosphono Acetic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results 2-Aminoethyl Phosphonic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Inconsistent results Methylene Diphosphonic acid	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Thymidine 3'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Thymidine 5'- Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: Growth Inositol Hexaphosphate	There are no media-specific metabolites present in the model. Optimization result: 0.0 (No growth) E. coli experimental result: No growth Thymidine 3', 5'-Cyclic Monophosphate
F	Optimization result: 22.0959 (Growth) E. coli experimental result: No growth sulfur negative control	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Sulfate	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Thiosulfate	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Tetrathionate	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Thiophosphate	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Dithiophosphate	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth L-Cysteine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth D-Cysteine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Cys-Gly	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Inconsistent results L-Cysteic acid	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Inconsistent results Cysteamine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth L-Cysteine Sulfinic acid
G	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: No growth N-Acetyl- L-Cysteine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Inconsistent results S-Methyl- L-Cysteine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Cystathionine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Lanthionine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Glutathione	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Inconsistent results DL-Ethionine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth L-Methionine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth D-Methionine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Gly-Met	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth N-Acetyl-D, L- Methionine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth L-Methionine Sulfoxide	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: No growth L-Methionine Sulfone
H	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth L-Djenkolic acid	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Inconsistent results Thiourea	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Inconsistent results 1-Thio- β-D- Glucose	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Inconsistent results DL-Lipoamide	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Inconsistent results Taurocholic acid	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Taurine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Hypotaurine	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: No growth p-Aminobenzene Sulfonic acid	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Butane Sulfonic acid	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth 2-Hydroxyethane Sulfonic acid	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: Growth Methane Sulfonic acid	There are no media-specific metabolites present in the model. Optimization result: 22.0959 (Growth) E. coli experimental result: No growth Tetramethylene Sulfone

Model: iAF1260.xml
Objective reaction: BIOMASS_Ec_iAF1260_core_59p81M
Extra metabolites, present in the objective reaction, included on every external media: ['ca2', 'cobalt2', 'cu2', 'mn2', 'mobd', 'zn2']
Extra metabolites, not present in the objective reaction, included on every external media: ['co2 (carbon dioxide)', 'o2 (oxygen)']
Extra metabolites included in the external media when testing carbon sources: []
Extra metabolites included in the external media when testing nitrogen sources: []
Extra metabolites included in the external media when testing phosphorus sources: []
Extra metabolites included in the external media when testing sulfur sources: []

Overview about the growth phenotype analisis

No growth on negative control	Total growth	Growth without media-specific metabolites	Total no growth	No growth without media-specific metabolites	FBA failed	True positive (if *E. coli model)	True negative (if *E. coli model)	True negative without media-specific metabolites (if *E. coli model)	False positive (if *E. coli model)	False negative (if *E. coli model)	False negative without media-specific metabolites (if *E. coli model)	Phenotype accuracy (if *E. coli model)
4 out of 4	156	0	227	189	0	114	123	97	27	73	67	70.3 %

Growth phenotype on carbon sources

No growth on negative control	Total growth	Growth without media-specific metabolites	Total no growth	No growth without media-specific metabolites	FBA failed	True positive (if *E. coli model)	True negative (if *E. coli model)	True negative without media-specific metabolites (if *E. coli model)	False positive (if *E. coli model)	False negative (if *E. coli model)	False negative without media-specific metabolites (if *E. coli model)	Phenotype accuracy (if *E. coli model)
Yes	73	0	118	103	0	55	84	69	16	27	27	76.4 %

Growth phenotype on nitrogen sources

No growth on negative control	Total growth	Growth without media-specific metabolites	Total no growth	No growth without media-specific metabolites	FBA failed	True positive (if *E. coli model)	True negative (if *E. coli model)	True negative without media-specific metabolites (if *E. coli model)	False positive (if *E. coli model)	False negative (if *E. coli model)	False negative without media-specific metabolites (if *E. coli model)	Phenotype accuracy (if *E. coli model)
Yes	41	0	55	41	0	17	28	19	11	15	15	63.4 %

Growth phenotype on phosphorus sources

No growth on negative control	Total growth	Growth without media-specific metabolites	Total no growth	No growth without media-specific metabolites	FBA failed	True positive (if *E. coli model)	True negative (if *E. coli model)	True negative without media-specific metabolites (if *E. coli model)	False positive (if *E. coli model)	False negative (if *E. coli model)	False negative without media-specific metabolites (if *E. coli model)	Phenotype accuracy (if *E. coli model)
Yes	33	0	27	26	0	33	6	5	0	17	17	69.6 %

Growth phenotype on sulfur sources

No growth on negative control	Total growth	Growth without media-specific metabolites	Total no growth	No growth without media-specific metabolites	FBA failed	True positive (if *E. coli model)	True negative (if *E. coli model)	True negative without media-specific metabolites (if *E. coli model)	False positive (if *E. coli model)	False negative (if *E. coli model)	False negative without media-specific metabolites (if *E. coli model)	Phenotype accuracy (if *E. coli model)
Yes	9	0	27	19	0	9	5	4	0	14	8	50.0 %

*Escherichia coli K-12 substr. MG1655

Biolog Phenotype Microarray Plate 1 - Carbon Sources

	1	2	3	4	5	6	7	8	9	10	11	12
A	Optimization result: 0.0 (No growth) E. coli experimental result: No growth Carbon negative control	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth L-Arabinose	Optimization result: 32.2675 (Growth) E. coli experimental result: Growth N-Acetyl-D- Glucosamine	Optimization result: 26.8844 (Growth) E. coli experimental result: Growth D-Saccharic acid	Optimization result: 20.2791 (Growth) E. coli experimental result: Growth Succinic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth D-Galactose	Optimization result: 16.6765 (Growth) E. coli experimental result: Growth L-Aspartic acid	Optimization result: 19.9838 (Growth) E. coli experimental result: Growth L-Proline	Optimization result: 18.9692 (Growth) E. coli experimental result: Growth D-Alanine	Optimization result: 33.4823 (Growth) E. coli experimental result: Growth D-Trehalose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth D-Mannose	Optimization result: 26.5835 (Growth) E. coli experimental result: Inconsistent results Dulcitol
B	Optimization result: 19.5995 (Growth) E. coli experimental result: Growth D-Serine	Optimization result: 26.8087 (Growth) E. coli experimental result: Growth D-Sorbitol	Optimization result: 23.8968 (Growth) E. coli experimental result: Growth Glycerol	Optimization result: 32.0723 (Growth) E. coli experimental result: Growth L-Fucose	Optimization result: 35.5086 (Growth) E. coli experimental result: Growth D-Glucuronic acid	Optimization result: 33.668 (Growth) E. coli experimental result: Growth D-Gluconic acid	Optimization result: 38.0421 (Growth) E. coli experimental result: Growth DL-α- Glycerol Phosphate	Optimization result: 29.4253 (Growth) E. coli experimental result: Growth D-Xylose	Optimization result: 18.9692 (Growth) E. coli experimental result: Growth L-Lactic acid	Optimization result: 0 (No growth) E. coli experimental result: No growth Formic acid	Optimization result: 26.8087 (Growth) E. coli experimental result: Growth D-Mannitol	Optimization result: 26.4364 (Growth) E. coli experimental result: Inconsistent results L-Glutamic acid
C	Optimization result: 44.0745 (Growth) E. coli experimental result: Growth D-Glucose- 6-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth D-Galactonic acid-γ- Lactone	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth DL-Malic acid	Optimization result: 29.4253 (Growth) E. coli experimental result: Growth D-Ribose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Tween 20	Optimization result: 32.0723 (Growth) E. coli experimental result: Growth L-Rhamnose	Optimization result: 29.4253 (Growth) E. coli experimental result: Growth D-Fructose	Optimization result: 9.1912 (Growth) E. coli experimental result: Growth Acetic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth α- D-Glucose	Optimization result: 44.0745 (Growth) E. coli experimental result: Growth Maltose	Optimization result: 33.4823 (Growth) E. coli experimental result: Growth D-Melibiose	Optimization result: 44.9868 (Growth) E. coli experimental result: Growth Thymidine
D	Optimization result: 13.54 (Growth) E. coli experimental result: Growth L-Asparagine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Aspartic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Glucosaminic acid	Optimization result: 15.0897 (Growth) E. coli experimental result: No growth 1,2-Propanediol	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Tween 40	Optimization result: 26.5334 (Growth) E. coli experimental result: Growth α- Ketoglutaric acid	Optimization result: 19.1279 (Growth) E. coli experimental result: Growth α- Ketobutyric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth α- Methyl-D- Galactoside	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth α- D-Lactose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Lactulose	Optimization result: 33.45 (Growth) E. coli experimental result: No growth Sucrose	Optimization result: 44.7789 (Growth) E. coli experimental result: Growth Uridine
E	Optimization result: 21.7707 (Growth) E. coli experimental result: Low growth L-Glutamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth M-Tartaric acid	Optimization result: 44.0745 (Growth) E. coli experimental result: Growth D-Glucose- 1-Phosphate	Optimization result: 44.0745 (Growth) E. coli experimental result: Growth D-Fructose- 6-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Tween 80	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth α- Hydroxyglutaric acid- γ-Lactone	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth α- Hydroxybutyric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth β- Methyl-D- Glucoside	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Adonitol	Optimization result: 41.178 (Growth) E. coli experimental result: Growth Maltotriose	Optimization result: 51.5223 (Growth) E. coli experimental result: Growth 2-Deoxyadenosine	Optimization result: 51.2978 (Growth) E. coli experimental result: Growth Adenosine
F	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Gly-Asp	Optimization result: 20.6445 (Growth) E. coli experimental result: No growth Citric acid	Optimization result: 0 (No growth) E. coli experimental result: No growth M-Inositol	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Threonine	Optimization result: 16.6765 (Growth) E. coli experimental result: Growth Fumaric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Bromosuccinic acid	Optimization result: 9.4446 (Growth) E. coli experimental result: Growth Propionic acid	Optimization result: 26.8844 (Growth) E. coli experimental result: Growth Mucic acid	Optimization result: 12.9343 (Growth) E. coli experimental result: Growth Glycolic acid	Optimization result: 13.8768 (Growth) E. coli experimental result: Growth Glyoxylic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Cellobiose	Optimization result: 48.2228 (Growth) E. coli experimental result: Growth Inosine
G	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Gly-Glu	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Tricarballylic acid	Optimization result: 19.5995 (Growth) E. coli experimental result: Growth L-Serine	Optimization result: 24.8527 (Growth) E. coli experimental result: Growth L-Threonine	Optimization result: 18.9692 (Growth) E. coli experimental result: Growth L-Alanine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Ala-Gly	Optimization result: 17.8353 (Growth) E. coli experimental result: Growth Acetoacetic acid	Optimization result: 32.2675 (Growth) E. coli experimental result: Growth N-Acetyl-D- Mannosamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Low growth Mono-Methylsuccinate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Methylpyruvate	Optimization result: 18.4748 (Growth) E. coli experimental result: Growth D-Malic acid	Optimization result: 20.4963 (Growth) E. coli experimental result: Growth L-Malic acid
H	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Gly-Pro	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth p-Hydroxyphenyl Acetic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth m-Hydroxyphenyl Acetic acid	Optimization result: 0 (No growth) E. coli experimental result: No growth Tyramine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results D-Psicose	Optimization result: 29.4253 (Growth) E. coli experimental result: Growth L-Lyxose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Glucuronamide	Optimization result: 18.9692 (Growth) E. coli experimental result: Growth Pyruvic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth L-Galactonic acid-γ- Lactone	Optimization result: 35.5086 (Growth) E. coli experimental result: Growth D-Galacturonic acid	Optimization result: 0 (No growth) E. coli experimental result: No growth Phenylethylamine	Optimization result: 18.4602 (Growth) E. coli experimental result: No growth 2-Aminoethanol

Biolog Phenotype Microarray Plate 2 - Carbon Sources

	1	2	3	4	5	6	7	8	9	10	11	12
A		There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Chondroitin Sulfate C	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth α- Cyclodextrin	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth β- Cyclodextrin	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth γ- Cyclodextrin	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Dextrin	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Gelatin	Optimization result: 44.0745 (Growth) E. coli experimental result: No growth Glycogen	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Inulin	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Laminarin	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Mannan	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Pectin
B	Optimization result: 0 (No growth) E. coli experimental result: No growth N-Acetyl-D- Galactosamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth N-Acetyl- Neuraminic acid	Optimization result: 29.4253 (Growth) E. coli experimental result: Growth β- D-Allose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Amygdalin	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Arabinose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Arabitol	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth L-Arabitol	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Arbutin	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth 2-Deoxy- D-Ribose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth i-Erythritol	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Fucose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth 3-O-β-D- Galactopyranosyl- D-Arabinose
C	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Gentiobiose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth L-Glucose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Lactitol	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Melezitose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Maltitol	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results α- Methyl-D- Glucoside	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth β-Methyl- D-Galactoside	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth 3-Methylglucose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth β- Methyl-D- Glucuronic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth α- Methyl-D- Mannoside	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth β- Methyl-D- Xyloside	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Palatinose
D	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Raffinose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Salicin	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Sedoheptulosan	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth L-Sorbose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Stachyose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Tagatose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Turanose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Xylitol	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth N-Acetyl-D- Glucosaminitol	Optimization result: 16.8318 (Growth) E. coli experimental result: No growth γ- Amino-N- Butyric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth δ- Amino Valeric acid	Optimization result: 13.8262 (Growth) E. coli experimental result: No growth Butyric acid
E	Optimization result: 14.1401 (Growth) E. coli experimental result: No growth Capric acid	Optimization result: 13.8262 (Growth) E. coli experimental result: No growth Caproic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Citraconic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Citramalic acid	Optimization result: 33.4803 (Growth) E. coli experimental result: Growth D-Glucosamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth 2-Hydroxybenzoic acid	Optimization result: 0 (No growth) E. coli experimental result: No growth 4-Hydroxybenzoic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth β- Hydroxybutyric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth γ- Hydroxybutyric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results α- Keto- Valeric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Itaconic acid	Optimization result: 36.2535 (Growth) E. coli experimental result: Growth 5-Keto-D- Gluconic acid
F	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth D-Lactic acid Methyl Ester	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Malonic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Melibionic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Oxalic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Oxalomalic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Quinic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Ribono- 1,4-Lactone	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Sebacic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Sorbic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Succinamic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Tartaric acid	Optimization result: 17.4973 (Growth) E. coli experimental result: No growth L-Tartaric acid
G	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Acetamide	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results L-Alaninamide	Optimization result: 14.4881 (Growth) E. coli experimental result: No growth N-Acetyl-L- Glutamic acid	Optimization result: 14.2549 (Growth) E. coli experimental result: No growth L-Arginine	Optimization result: 14.679 (Growth) E. coli experimental result: No growth Glycine	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Histidine	Optimization result: 29.6568 (Growth) E. coli experimental result: No growth L-Homoserine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Hydroxy- L-Proline	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Isoleucine	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Leucine	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Lysine	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Methionine
H	Optimization result: 14.843 (Growth) E. coli experimental result: No growth L-Ornithine	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Phenylalanine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth L-Pyroglutamic acid	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Valine	Optimization result: 0 (No growth) E. coli experimental result: No growth D,L-Carnitine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth sec-Butylamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D,L-Octopamine	Optimization result: 14.3799 (Growth) E. coli experimental result: No growth Putrescine	Optimization result: 28.749 (Growth) E. coli experimental result: Growth Dihydroxyacetone	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth 2,3-Butanediol	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth 2,3-Butanedione	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth 3-Hydroxy- 2-butanone

Biolog Phenotype Microarray Plate 3 - Nitrogen Sources

	1	2	3	4	5	6	7	8	9	10	11	12
A	Optimization result: 0.0 (No growth) E. coli experimental result: No growth nitrogen negative control	Optimization result: 18.9692 (Growth) E. coli experimental result: Growth Ammonia	Optimization result: 18.9692 (Growth) E. coli experimental result: No growth Nitrite	Optimization result: 18.9692 (Growth) E. coli experimental result: No growth Nitrate	Optimization result: 0 (No growth) E. coli experimental result: No growth Urea	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Biuret	Optimization result: 25.3986 (Growth) E. coli experimental result: Growth L-Alanine	Optimization result: 22.8636 (Growth) E. coli experimental result: Growth L-Arginine	Optimization result: 22.5936 (Growth) E. coli experimental result: Growth L-Asparagine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth L-Aspartic acid	Optimization result: 22.0444 (Growth) E. coli experimental result: Inconsistent results L-Cysteine	Optimization result: 32.9883 (Growth) E. coli experimental result: Inconsistent results L-Glutamic acid
B	Optimization result: 27.9367 (Growth) E. coli experimental result: Growth L-Glutamine	Optimization result: 22.5176 (Growth) E. coli experimental result: Growth Glycine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth L-Histidine	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Isoleucine	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Leucine	Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results L-Lysine	Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results L-Methionine	Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results L-Phenylalanine	Optimization result: 28.2151 (Growth) E. coli experimental result: Growth L-Proline	Optimization result: 26.4357 (Growth) E. coli experimental result: Growth L-Serine	Optimization result: 31.7265 (Growth) E. coli experimental result: Inconsistent results L-Threonine	Optimization result: 21.8655 (Growth) E. coli experimental result: Growth L-Tryptophan
C	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Tyrosine	Optimization result: 0 (No growth) E. coli experimental result: No growth L-Valine	Optimization result: 25.3986 (Growth) E. coli experimental result: Growth D-Alanine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results D-Asparagine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Aspartic acid	Optimization result: 32.9883 (Growth) E. coli experimental result: No growth D-Glutamic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results D-Lysine	Optimization result: 26.4357 (Growth) E. coli experimental result: Growth D-Serine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results D-Valine	Optimization result: 23.9626 (Growth) E. coli experimental result: No growth L-Citrulline	Optimization result: 36.8015 (Growth) E. coli experimental result: Inconsistent results L-Homoserine	Optimization result: 23.9626 (Growth) E. coli experimental result: Growth L-Ornithine
D	Optimization result: 22.4721 (Growth) E. coli experimental result: No growth N-Acetyl-L- Glutamic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth N-Phthaloyl-L- Glutamic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth L-Pyroglutamic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Hydroxylamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Methylamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth N-Amylamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth N-Butylamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Ethylamine	Optimization result: 27.0344 (Growth) E. coli experimental result: No growth Ethanolamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Ethylenediamine	Optimization result: 23.9144 (Growth) E. coli experimental result: Inconsistent results Putrescine	Optimization result: 23.3944 (Growth) E. coli experimental result: Inconsistent results Agmatine
E	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Histamine	Optimization result: 18.9692 (Growth) E. coli experimental result: No growth b-Phenylethylamine	Optimization result: 18.9692 (Growth) E. coli experimental result: No growth Tyramine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Acetamide	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Formamide	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Glucuronamide	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth DL-Lactamide	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth D-Glucosamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth D-Galactosamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results D-Mannosamine	Optimization result: 33.1547 (Growth) E. coli experimental result: Growth N-Acetyl-D- Glucosamine	Optimization result: 0 (No growth) E. coli experimental result: No growth N-Acetyl-D- Galactosamine
F	Optimization result: 33.1547 (Growth) E. coli experimental result: Inconsistent results N-Acetyl-D- Mannosamine	Optimization result: 20.4514 (Growth) E. coli experimental result: Inconsistent results Adenine	Optimization result: 53.5312 (Growth) E. coli experimental result: Growth Adenosine	Optimization result: 51.8051 (Growth) E. coli experimental result: Growth Cytidine	Optimization result: 19.1502 (Growth) E. coli experimental result: Growth Cytosine	Optimization result: 20.491 (Growth) E. coli experimental result: No growth Guanine	Optimization result: 53.6667 (Growth) E. coli experimental result: Inconsistent results Guanosine	Optimization result: 0 (No growth) E. coli experimental result: No growth Thymine	Optimization result: 0 (No growth) E. coli experimental result: No growth Thymidine	Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Uracil	Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Uridine	Optimization result: 48.5555 (Growth) E. coli experimental result: No growth Inosine
G	Optimization result: 20.3989 (Growth) E. coli experimental result: Inconsistent results Xanthine	Optimization result: 48.3163 (Growth) E. coli experimental result: Inconsistent results Xanthosine	Optimization result: 19.6742 (Growth) E. coli experimental result: Inconsistent results Uric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Alloxan	Optimization result: 19.9711 (Growth) E. coli experimental result: No growth Allantoin	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Parabanic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results DL-α- Amino-N- Butyric acid	Optimization result: 25.3184 (Growth) E. coli experimental result: Inconsistent results γ- Amino-N- Butyric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth ε- Amino-N- Caproic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results DL-α- Amino- Caprylic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth δ- Amino-N- Valeric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth α- Amino-N- Valeric acid
H	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Ala-Asp	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Ala-Gln	Optimization result: 27.6038 (Growth) E. coli experimental result: Growth Ala-Glu	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Ala-Gly	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Ala-His	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Ala-Leu	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Ala-Thr	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Gly-Asn	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Gly-Gln	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Gly-Glu	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Gly-Met	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Met-Ala

Biolog Phenotype Microarray Plate 4 - Phosphorus and Sulfur Sources

	1	2	3	4	5	6	7	8	9	10	11	12
A	Optimization result: 0.0 (No growth) E. coli experimental result: No growth phosphorus negative control	Optimization result: 18.9692 (Growth) E. coli experimental result: Growth Phosphate	Optimization result: 23.8406 (Growth) E. coli experimental result: Growth Pyrophosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Trimetaphosphate	Optimization result: 25.6988 (Growth) E. coli experimental result: Growth Tripolyphosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Triethyl Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Hypophosphite	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Adenosine 2'- Monophosphate	Optimization result: 47.8881 (Growth) E. coli experimental result: Growth Adenosine 3'- Monophosphate	Optimization result: 51.2416 (Growth) E. coli experimental result: Growth Adenosine 5'- Monophosphate	Optimization result: 38.3162 (Growth) E. coli experimental result: Growth Adenosine 2', 3'-Cyclic Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Adenosine 3', 5'-Cyclic Monophosphate
B	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Thiophosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Dithiophosphate	Optimization result: 47.3776 (Growth) E. coli experimental result: Growth DL-α- Glycerol Phosphate	Optimization result: 35.8275 (Growth) E. coli experimental result: Growth β- Glycerol Phosphate	Optimization result: 21.6896 (Growth) E. coli experimental result: Growth Carbamyl Phosphate	Optimization result: 38.4479 (Growth) E. coli experimental result: Growth D-2-Phospho- Glyceric acid	Optimization result: 38.4479 (Growth) E. coli experimental result: Growth D-3-Phospho- Glyceric acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Guanosine 2'- Monophosphate	Optimization result: 48.0121 (Growth) E. coli experimental result: Growth Guanosine 3'- Monophosphate	Optimization result: 51.3836 (Growth) E. coli experimental result: Growth Guanosine 5'- Monophosphate	Optimization result: 38.4166 (Growth) E. coli experimental result: Growth Guanosine 2', 3'-Cyclic Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Guanosine 3', 5'-Cyclic Monophosphate
C	Optimization result: 31.0097 (Growth) E. coli experimental result: Growth Phosphoenol Pyruvate	Optimization result: 23.2989 (Growth) E. coli experimental result: Growth Phospho- Glycolic acid	Optimization result: 48.3719 (Growth) E. coli experimental result: Growth D-Glucose- 1-Phosphate	Optimization result: 48.3719 (Growth) E. coli experimental result: Growth D-Glucose- 6-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth 2-Deoxy-D- Glucose 6- Phosphate	Optimization result: 46.4087 (Growth) E. coli experimental result: Growth D-Glucosamine- 6-Phosphate	Optimization result: 48.3719 (Growth) E. coli experimental result: Growth 6-Phospho- Gluconic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Cytidine 2'- Monophosphate	Optimization result: 42.9738 (Growth) E. coli experimental result: Growth Cytidine 3'- Monophosphate	Optimization result: 48.9392 (Growth) E. coli experimental result: Growth Cytidine 5'- Monophosphate	Optimization result: 34.6223 (Growth) E. coli experimental result: Growth Cytidine 2', 3'-Cyclic Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Cytidine 3', 5'-Cyclic Monophosphate
D	Optimization result: 48.3719 (Growth) E. coli experimental result: Growth D-Mannose- 1-Phosphate	Optimization result: 48.3719 (Growth) E. coli experimental result: Growth D-Mannose- 6-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Cysteamine- S-Phosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Phospho- L-Arginine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth O-Phospho- D-Serine	Optimization result: 22.5176 (Growth) E. coli experimental result: Growth O-Phospho- L-Serine	Optimization result: 26.8152 (Growth) E. coli experimental result: Growth O-Phospho- L-Threonine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Uridine 2'- Monophosphate	Optimization result: 46.3505 (Growth) E. coli experimental result: Growth Uridine 3'- Monophosphate	Optimization result: 48.4932 (Growth) E. coli experimental result: Growth Uridine 5'- Monophosphate	Optimization result: 36.5465 (Growth) E. coli experimental result: Growth Uridine 2', 3'-Cyclic Monophosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Uridine 3', 5'-Cyclic Monophosphate
E	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth O-Phospho- D-Tyrosine	Optimization result: 19.282 (Growth) E. coli experimental result: Growth O-Phospho- L-Tyrosine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Phosphocreatine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Phosphoryl Choline	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth O-Phosphoryl- Ethanolamine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Phosphono Acetic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results 2-Aminoethyl Phosphonic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Methylene Diphosphonic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Thymidine 3'- Monophosphate	Optimization result: 46.8487 (Growth) E. coli experimental result: Growth Thymidine 5'- Monophosphate	Optimization result: 18.9692 (Growth) E. coli experimental result: Growth Inositol Hexaphosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Thymidine 3', 5'-Cyclic Monophosphate
F	Optimization result: 0.0 (No growth) E. coli experimental result: No growth sulfur negative control	Optimization result: 18.9692 (Growth) E. coli experimental result: Growth Sulfate	Optimization result: 0 (No growth) E. coli experimental result: Growth Thiosulfate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Tetrathionate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Thiophosphate	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Dithiophosphate	Optimization result: 22.0444 (Growth) E. coli experimental result: Growth L-Cysteine	Optimization result: 21.8655 (Growth) E. coli experimental result: Growth D-Cysteine	Optimization result: 21.9452 (Growth) E. coli experimental result: Growth Cys-Gly	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results L-Cysteic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Cysteamine	Optimization result: 0 (No growth) E. coli experimental result: Growth L-Cysteine Sulfinic acid
G	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth N-Acetyl- L-Cysteine	Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results S-Methyl- L-Cysteine	Optimization result: 0 (No growth) E. coli experimental result: Growth Cystathionine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Lanthionine	Optimization result: 27.7508 (Growth) E. coli experimental result: Growth Glutathione	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results DL-Ethionine	Optimization result: 0 (No growth) E. coli experimental result: Growth L-Methionine	Optimization result: 0 (No growth) E. coli experimental result: Growth D-Methionine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Gly-Met	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth N-Acetyl-D, L- Methionine	Optimization result: 0 (No growth) E. coli experimental result: Growth L-Methionine Sulfoxide	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth L-Methionine Sulfone
H	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth L-Djenkolic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Thiourea	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results 1-Thio- β-D- Glucose	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results DL-Lipoamide	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Inconsistent results Taurocholic acid	Optimization result: 19.0579 (Growth) E. coli experimental result: Growth Taurine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: Growth Hypotaurine	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth p-Aminobenzene Sulfonic acid	Optimization result: 22.8011 (Growth) E. coli experimental result: Growth Butane Sulfonic acid	Optimization result: 21.8248 (Growth) E. coli experimental result: Growth 2-Hydroxyethane Sulfonic acid	Optimization result: 19.1036 (Growth) E. coli experimental result: Growth Methane Sulfonic acid	There are no media-specific metabolites present in the model. Optimization result: 0 (No growth) E. coli experimental result: No growth Tetramethylene Sulfone

Reactions

Exchange reactions plus sink reactions

	Intersection of exchange plus sink reactions
e_coli_core in iAF1260	7 (100% of exchange plus sink reaction on e_coli_core
iAF1260 in e_coli_core	7 (31.8% of exchange plus sink reaction on iAF1260

Using data of exchange reactions plus sink reactions of e_coli_core in iAF1260

Metabolic reactions intersection

	Intersection of metabolic reactions
e_coli_core in iAF1260	48 (96% of metabolic reaction on e_coli_core
iAF1260 in e_coli_core	50 (3.7% of metabolic reaction on iAF1260

Using data of metabolic reactions of e_coli_core in iAF1260

Growth phenotype comparison

For each culture medium, the growth/no-growth prediction is compared for both models. A high intersection in the Veen diagram indicates a high phenotype match of the two models.
Media with no growth for both models: 200
Media with growth for both models: 24
Media with growth only for e_coli_core: 27
Media with growth only for iAF1260: 132

Modules/Pathways comparison

Only modules/pathways with a minimum of 30% completeness, and at least 4 reactions, are shown.

KEGG

BioCyc

Models distance based on the metabolic reactions similarity

Distance matrix (based on the Jaccard distance coefficient)

	e_coli_core	iAF1260
e_coli_core	0	0.9645756457564575
iAF1260	0.9645756457564575	0

Distance visualization

Multidimensional scaling plots display the relative distances of objects. One metric of how well their relative distances are represented in the plot is the "Kruskal stress". Kruskal (1964) suggests the following interpretation of the stress: 0.000 perfect fit; 0.025 excellent fit; 0.050 good fit; 0.100 fair fit; 0.200 poor fit.

Models distance based on the exchange reactions similarity

Distance matrix (based on the Jaccard distance coefficient)

	e_coli_core	iAF1260
e_coli_core	0	0.6818181818181819
iAF1260	0.6818181818181819	0

Distance visualization

Multidimensional scaling plots display the relative distances of objects. One metric of how well their relative distances are represented in the plot is the "Kruskal stress". Kruskal (1964) suggests the following interpretation of the stress: 0.000 perfect fit; 0.025 excellent fit; 0.050 good fit; 0.100 fair fit; 0.200 poor fit.

Models distance based on the growth phenotype similarity

Distance matrix (based on the Jaccard distance coefficient)

	e_coli_core	iAF1260
e_coli_core	0	0.4151436031331593
iAF1260	0.4151436031331593	0

BioCyc Pathways from iAF1260

BioCyc Pathways from e_coli_core

No BioCyc Modules to include

Include demand reactions to unblock reactions

SBMLComp

Model Analysis

General characteristics

Selected organism

Compartments

Metabolites

Reactions

Reactions Types

Metabolic reactions blocked

Metabolic reactions synonyms on other databases

KEGG Pathway Modules

Tables (with clickable links)

Heatmap

BioCyc Pathways

Tables

Heatmap

Growth phenotype

Pairwise Model Comparison

e_coli_core and iAF1260

Reactions

Exchange reactions plus sink reactions

Metabolic reactions intersection

Growth phenotype comparison

Modules/Pathways comparison

KEGG

BioCyc

Models distance

Models distance based on the metabolic reactions similarity

Distance matrix (based on the Jaccard distance coefficient)

Distance visualization

Models distance based on the exchange reactions similarity

Distance matrix (based on the Jaccard distance coefficient)

Distance visualization

Models distance based on the growth phenotype similarity

Distance matrix (based on the Jaccard distance coefficient)

Distance visualization

Export models

e_coli_core

Include in the model reactions annotated by KEGG and BioCyc for the selected organism

KEGG Modules

BioCyc Pathways

Remove from model reactions annotated by KEGG and BioCyc on the selected organism

KEGG Modules

BioCyc Pathways

Remove from model reactions mapped to KEGG and BioCyc pathways NOT annotated on the selected organism

KEGG Modules

BioCyc Pathways

Remove from model reactions mapped to KEGG and BioCyc pathways NOT annotated on the selected organism, and can not contribute to maximum growth

KEGG Modules

BioCyc Pathways

Include reactions from another model

KEGG Modules from iAF1260

BioCyc Pathways from iAF1260

iAF1260

Include in the model reactions annotated by KEGG and BioCyc for the selected organism

KEGG Modules

BioCyc Pathways

Remove from model reactions annotated by KEGG and BioCyc on the selected organism

KEGG Modules

BioCyc Pathways

Remove from model reactions mapped to KEGG and BioCyc pathways NOT annotated on the selected organism

KEGG Modules

BioCyc Pathways

Remove from model reactions mapped to KEGG and BioCyc pathways NOT annotated on the selected organism, and can not contribute to maximum growth

KEGG Modules

BioCyc Pathways

Include reactions from another model

KEGG Modules from e_coli_core

BioCyc Pathways from e_coli_core